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公开(公告)号：US11542540B2
公开(公告)日：2023-01-03
申请号：US16622113
申请日：2018-06-12
发明人： Dalia Dhingra , Richard Chien
IPC分类号： C12Q1/68 , C12Q1/6806 , C12Q1/6869
摘要： Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.
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公开(公告)号：US20220412890A1
公开(公告)日：2022-12-29
申请号：US17825222
申请日：2022-05-26
发明人： Kevin MAHER , Mingsong CHEN , Jacob K. FREUDENTHAL , Kuan Moon BOO , Soo Yong LAU , David FORTESCUE , Ming SHEN , Woon Liang Terence SOH
IPC分类号： G01N21/64
摘要： An instrument for processing and/or measuring a biological process comprises a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.
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公开(公告)号：US11513133B2
公开(公告)日：2022-11-29
申请号：US16194187
申请日：2018-11-16
摘要： The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.
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公开(公告)号：US20220372435A1
公开(公告)日：2022-11-24
申请号：US17761879
申请日：2020-09-17
发明人： Mwita PHELPS
IPC分类号： C12N5/00
摘要： Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.
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公开(公告)号：US11505827B2
公开(公告)日：2022-11-22
申请号：US16351456
申请日：2019-03-12
发明人： Timothy Geiser , Randall Davis , Roger Chen
IPC分类号： C12M1/34 , C12Q1/6874 , C12Q1/6869 , B82Y15/00
摘要： Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.
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公开(公告)号：US20220349807A1
公开(公告)日：2022-11-03
申请号：US17729440
申请日：2022-04-26
发明人： Eric Finan , Michael Ward
IPC分类号： G01N15/14
摘要： Provided are, inter alia, flow centration components that can be used in flow cytometers and other applications. A flow centrator can define central axis, a proximal end, and a distal end, and having a central bore extending within the flow centration component in the direction of the central axis; the flow centration component defining a splined outer surface that defines a plurality of circumferentially arranged bypass flute channels, the plurality of bypass flute channels extending in the direction of the central axis, and each of the bypass flute channels having a depth and a length. Also provided are related methods that utilize the disclosed components.
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公开(公告)号：US20220348964A1
公开(公告)日：2022-11-03
申请号：US17860833
申请日：2022-07-08
发明人： Xin YU , Xavier de MOLLERAT du JEU
摘要： A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.
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公开(公告)号：US11479814B2
公开(公告)日：2022-10-25
申请号：US16866211
申请日：2020-05-04
发明人： Parul Angrish , Zhiwei Yang
IPC分类号： C12Q1/68 , C12Q1/686 , C07D207/16 , C07C41/22 , C07C211/63 , C07C303/28 , C07D211/60 , C11D1/88 , C12N9/12 , C12N9/96
摘要： This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.
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79.发明授权公开(公告)号：US11474070B2
公开(公告)日：2022-10-18
申请号：US16196502
申请日：2018-11-20
发明人： Marcin Sikora , Melville Davey , Christian Koller , Simon Cawley , Alan Williams , David Kulp
IPC分类号： G01N27/414 , G16B30/00 , G16B30/10 , C12Q1/6869 , G01N27/27 , G16B30/20
摘要： A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.
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公开(公告)号：US11473132B2
公开(公告)日：2022-10-18
申请号：US16948442
申请日：2020-09-18
发明人： Shoulian Dong , Chunmei Liu
IPC分类号： C12Q1/6855 , C12P19/34 , C12Q1/6851 , C12Q1/6858 , C12Q1/686 , C12Q1/6853 , C12Q1/6832 , C12Q1/6848
摘要： The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
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