公开(公告)号：US4908308A

公开(公告)日：1990-03-13

申请号：US746282

申请日：1985-06-19

Applicant: Lex H. T. Van der Ploeg ,  Suzanne H. Giannini ,  Charles R. Cantor

Inventor： Lex H. T. Van der Ploeg ,  Suzanne H. Giannini ,  Charles R. Cantor

IPC: C12Q1/68

CPC classification number: C12Q1/6893 ,  C12Q2600/158

Abstract: This invention concerns a method for identifying in vitro an animal-infective form of a parasitic protozoan which comprises recovering total mRNA from the protozoan and detecting in the mRNA so recovered the presence of a mRNA transcript encoding a heat shock protein associated with animal-infective parasitic protozoans, which transcript is present only in the animal-infective form of the protozoan, or quantitatively determining in the mRNA so recovered the number of a mRNA transcript encoding a heat shock protein associated with animal-infective parasitic protozoans, which transcript is present in increased number only in the animal-infective form of the parasitic protozoan.This invention also concerns a method for identifying an agent capable of blocking the formation of the animal-infective form of a parasitic protozoan.

Abstract translation: 本发明涉及用于体外鉴定寄生原生动物的动物感染形式的方法，其包括从原生动物中回收总mRNA并检测mRNA，从而回收编码与动物感染性寄生虫有关的热休克蛋白的mRNA转录物的存在 原生动物，其转录物仅存在于原生动物的动物感染形式中，或者在mRNA中定量测定，从而回收编码与动物感染性寄生原生动物相关的热休克蛋白的mRNA转录物的数目，该转录物存在于增加的 数量仅在寄生原生动物的动物感染形式中。 本发明还涉及鉴定能够阻断寄生原生动物的动物感染形式形成的试剂的方法。

公开(公告)号：US4473452A

公开(公告)日：1984-09-25

申请号：US442580

申请日：1982-11-18

Applicant: Charles R. Cantor ,  David C. Schwartz

Inventor： Charles R. Cantor ,  David C. Schwartz

IPC: G01N27/26 ,  B01D20060101 ,  B01D57/02 ,  G01N20060101 ,  G01N27/447 ,  G01N27/28

CPC classification number: G01N27/44773

Abstract: Disclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low intensities out of phase with each other at a frequency related to the mass of the particles and which move the particles in an overall direction transverse to the respective directions of the fields. For separating large macromolecules, at least one of the fields preferably has an intensity gradient in a direction transverse to its own. The new arrangement makes it possible to: (1) separate particles (molecules) larger in size than those able to be separated with previously known techniques, (2) carry out separation at higher speed and at better resolution than is possible with previously known techniques, and (3) concurrently separate particles which differ greatly in mass (molecular weight).

Abstract translation: 公开了一种通过电场电泳分离颗粒的装置和方法，它们彼此横向，它们以相对于颗粒的质量的频率彼此相位的相应的高和低强度之间交替， 颗粒在横向于各个方向的整个方向上。 为了分离大的大分子，至少一个场优选在横向于其自身的方向上具有强度梯度。 新的布置使得可以：（1）分离比以前已知技术更大尺寸的颗粒（分子），（2）以比先前已知技术更高的速度和更好的分辨率进行分离 ，（3）同时分离质量差异很大的分子（分子量）。

公开(公告)号：US10533215B2

公开(公告)日：2020-01-14

申请号：US13129797

申请日：2009-11-20

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C12Q1/68 ,  C12Q1/6837

Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

公开(公告)号：US09376714B2

公开(公告)日：2016-06-28

申请号：US13412984

申请日：2012-03-06

Applicant: Charles R Cantor ,  Chunming Ding

Inventor： Charles R Cantor ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

公开(公告)号：US08852864B2

公开(公告)日：2014-10-07

申请号：US12863169

申请日：2009-01-16

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/185

Abstract: Provided herein are compositions and methods for analysis of nucleic acids, including, methods and compositions for genotyping, haplotyping, sequencing and performing other genetic and epigenetic analysis on nucleic acids, for example. In some embodiments, methods and compositions suitable for whole-genome sequencing on single molecules of nucleic acid are provided. In some embodiments, analysis of single molecules of nucleic acid are performed in conjunction with nanopores and/or nanopore devices.

Abstract translation: 本文提供了用于核酸分析的组合物和方法，包括用于基因分型，单倍型，测序和对核酸进行其他遗传和表观遗传学分析的方法和组合物。 在一些实施方案中，提供适用于单分子核酸上的全基因组测序的方法和组合物。 在一些实施方案中，结合纳米孔和/或纳米孔装置进行单分子核酸的分析。

公开(公告)号：US08821816B2

公开(公告)日：2014-09-02

申请号：US12123378

申请日：2008-05-19

Applicant: Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

Inventor： Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

IPC: G01N37/00 ,  H01J49/04 ,  B01L3/02

CPC classification number: H01J49/0418 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00468 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  C07B2200/11 ,  C07F9/2408 ,  C07F9/2429 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N35/1067 ,  G01N35/1074 ,  G01N2035/1037 ,  G01N2035/1069 ,  Y10T428/26 ,  Y10T428/31678 ,  Y10T428/31855 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.

Abstract translation: 本文提供了用于基质辅助激光解吸电离（MALDI）质谱分析的底物。 每个斑点包括3-羟基吡啶甲酸基质，无分析物。

公开(公告)号：US08758995B2

公开(公告)日：2014-06-24

申请号：US12852336

申请日：2010-08-06

Applicant: Charles R. Cantor ,  Hubert Koster

Inventor： Charles R. Cantor ,  Hubert Koster

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6872 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2531/113 ,  C12Q2521/525 ,  C12Q2565/627 ,  C12Q2531/119 ,  C12Q2523/301 ,  C12Q2531/137 ,  C12Q2521/301 ,  C12Q2531/149

Abstract: This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Probes may be affixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

Abstract translation: 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 探针可以固定在诸如杂交芯片的固体支持物上，以便于自动化分子量分析和目标序列的鉴定。

公开(公告)号：US20120295263A1

公开(公告)日：2012-11-22

申请号：US13565105

申请日：2012-08-02

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: G01N27/62

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

公开(公告)号：US20120225798A1

公开(公告)日：2012-09-06

申请号：US13369000

申请日：2012-02-08

Applicant: Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

Inventor： Charles R. Cantor ,  Chunming Ding ,  Yuk Ming Dennis Lo ,  Rossa Wai Kwum Chiu

IPC: C40B30/10

CPC classification number: C12Q1/6881 ,  C12Q1/6872 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2535/125

Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.

Abstract translation: 本发明涉及检测生物样品中核酸的方法。 该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。 该方法可用于例如诊断，预后和治疗目的。 该方法允许精确检测在生物样品中以非常少的量存在的核酸。 例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性核酸。 在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。

公开(公告)号：US07999095B2

公开(公告)日：2011-08-16

申请号：US12638871

申请日：2009-12-15

Applicant: Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

Inventor： Charles R. Cantor ,  Natalia E. Broude ,  Carlos Witte-Hoffman

IPC: C07H21/04 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6818 ,  C12Q1/6813 ,  C12Q2563/131 ,  C12Q2561/107

Abstract: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.

Abstract translation: 本发明涉及用于靶核酸分子（包括DNA和RNA靶标）以及核酸类似物的体外和体内检测的新方法。 本发明基于蛋白质互补，其中两个单独的多肽是无活性的。 当两个无活性多肽片段在与靶核酸杂交期间紧密接近时，它们重新连接成活性的可检测蛋白质。