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98 条记录 (耗时 0.037 秒)

    Gene isolation method
    72.
    发明授权
    Gene isolation method 失效
    基因分离方法

    公开(公告)号：US06770481B1

    公开(公告)日：2004-08-03

    申请号：US09690885

    申请日：2000-10-18

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    IPC分类号： C12N1563

    CPC分类号： C12N15/69 ,  C12N9/1007 ,  C12N9/22

    摘要： A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

    摘要翻译： 一种质粒载体，其特征在于包含可以通过RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和复制起点，其通过外源因子诱导的拷贝数增加; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。 本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法 即使在不存在修饰酶基因的情况下也构成即将分离的限制性修饰系统的内切核酸酶 - 基因，这在现有技术中是困难的。

    Therapeutic agents
    74.
    发明授权
    Therapeutic agents 失效
    治疗剂

    公开(公告)号：US06492338B1

    公开(公告)日：2002-12-10

    申请号：US09720308

    申请日：2000-12-26

    申请人： Hiroaki Sagawa ,  Harumi Ueno ,  Kaori Akiyama ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Harumi Ueno ,  Kaori Akiyama ,  Ikunoshin Kato

    IPC分类号： A61K3170

    CPC分类号： C07D307/24 ,  A61K31/00 ,  A61K31/341

    摘要： Therapeutic or preventive agents for diseases due to the action of aldose reductase; and aldose reductase inhibitors, characterized by containing at least one compound having an aldose reductase inhibiting activity which is selected from among 2,5-dihydroxytetra-hydro-2-furancarboxylic acid, derivatives of the same, optical isomers of both and pharmacologically acceptable salts of them.

    摘要翻译： 由于醛糖还原酶的作用引起的疾病的治疗或预防剂; 和醛糖还原酶抑制剂，其特征在于含有至少一种具有醛糖还原酶抑制活性的化合物，其选自2,5-二羟基四氢-2-呋喃甲酸，其衍生物，两者的光学异构体和药理学上可接受的盐 他们。

    Antitumor Agent
    78.
    发明申请
    Antitumor Agent 审中-公开
    抗肿瘤剂

    公开(公告)号：US20080038289A1

    公开(公告)日：2008-02-14

    申请号：US11596395

    申请日：2005-05-30

    申请人： Takashi Kawai ,  Shigetoshi Mizutani ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Kazuo Shimanaka ,  Ikunoshin Kato

    发明人： Takashi Kawai ,  Shigetoshi Mizutani ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Kazuo Shimanaka ,  Ikunoshin Kato

    IPC分类号： A61K36/06 ,  A61K31/045 ,  A61P35/00

    CPC分类号： A61K36/07 ,  A23L33/105 ,  A61K31/047

    摘要： According to the present invention, an antitumor agent, an apoptosis-inducing agent or an angiogenesis-suppressing agent having the effect even via an oral route, containing a compound contained in an inexpensive edible basidiomycete or a composition derived from an edible basidiomycete, and a food having the antitumor activity, the apoptosis-inducing activity or the angiogenesis-suppressing activity containing the compound or the composition can be obtained. Therefore, the present invention is particularly useful, especially in the field of a medicine and a health food.

    摘要翻译： 根据本发明，通过口服途径具有效果的抗肿瘤剂，细胞凋亡诱导剂或血管生成抑制剂，其含有廉价的可食用担子菌或含有可食用担子菌的组合物，以及 可以获得具有抗肿瘤活性，含有化合物或组合物的细胞凋亡诱导活性或血管生成抑制活性的食品。 因此，本发明特别有用，特别是在药物和保健食品领域。

    Agarase and gene thereof
    79.
    发明授权
    Agarase and gene thereof 失效
    琼脂糖酶及其基因

    公开(公告)号：US07329524B2

    公开(公告)日：2008-02-12

    申请号：US10467824

    申请日：2002-02-26

    申请人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12N9/24 ,  C12N9/40 ,  C12N1/21 ,  C12N15/63 ,  C12P19/26 ,  C07H21/04

    CPC分类号： C12Y302/01081 ,  C12N9/2468

    摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

    摘要翻译： 高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

    Remedies
    80.
    发明授权
    Remedies 失效
    补救措施

    公开(公告)号：US07268160B2

    公开(公告)日：2007-09-11

    申请号：US10182193

    申请日：2001-01-26

    申请人： Hiromu Ohnogi ,  Masahiro Shiraga ,  Eiji Kobayashi ,  Tuo-Ping Li ,  Suzu Deguchi ,  Eiji Nishiyama ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Hiromu Ohnogi ,  Masahiro Shiraga ,  Eiji Kobayashi ,  Tuo-Ping Li ,  Suzu Deguchi ,  Eiji Nishiyama ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： A61K31/35 ,  A61K31/22 ,  C07D307/02 ,  C07C67/02

    CPC分类号： A61K31/7004 ,  A23L33/10 ,  A61K31/12 ,  A61K31/216 ,  A61K31/222 ,  A61K31/352

    摘要： The present invention provides a therapeutic agent or prophylactic agent for a disease that requires enhancement of nerve growth factor production, an enhancer for nerve growth factor production, or a food, beverage or feed for enhancing nerve growth factor production, each comprising as an effective ingredient a specified compound or a salt thereof having enhancing activity for nerve growth factor production; a method for enhancing nerve growth factor production, comprising administering to a mammal the above-mentioned compound or a salt thereof; and use of the above-mentioned compound or a salt thereof in the preparation of a therapeutic agent or prophylactic agent for a disease that requires enhancement of nerve growth factor production, an enhancer for nerve growth factor production, or a food, beverage or feed for enhancing nerve growth factor production. In addition, the present invention provides novel compounds having enhancing activity for nerve growth factor production.

    摘要翻译： 本发明提供了需要增强神经生长因子产生的疾病的治疗剂或预防剂，用于神经生长因子产生的增强剂或用于增强神经生长因子产生的食品，饮料或饲料，其各自包含作为有效成分 具有增强神经生长因子生成活性的特定化合物或其盐; 一种用于增强神经生长因子产生的方法，包括向哺乳动物施用上述化合物或其盐; 以及上述化合物或其盐在制备需要增强神经生长因子产生的疾病的治疗剂或预防剂，用于神经生长因子产生的增强剂或食品，饮料或饲料中的用途 增强神经生长因子的产生。 此外，本发明提供了具有增强神经生长因子产生活性的新型化合物。