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    Culture medium containing gamma-aminobutyric acid, pipecolic acid or lithium for the maintenance of stem cells in an undifferentiated state
    72.
    发明授权
    Culture medium containing gamma-aminobutyric acid, pipecolic acid or lithium for the maintenance of stem cells in an undifferentiated state 有权
    含有γ-氨基丁酸，哌啶酸或锂的培养基用于维持未分化状态的干细胞

    公开(公告)号：US08426203B2

    公开(公告)日：2013-04-23

    申请号：US13427548

    申请日：2012-03-22

    申请人： James A. Thomson ,  Tenneille Ludwig

    发明人： James A. Thomson ,  Tenneille Ludwig

    IPC分类号： C12N5/04

    CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

    摘要： Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

    摘要翻译： 培养灵长类多能干细胞的先前方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。

    MEDIUM AND CULTURE OF EMBRYONIC STEM CELLS
    73.
    发明申请
    MEDIUM AND CULTURE OF EMBRYONIC STEM CELLS 有权
    胚胎干细胞的培养和培养

    公开(公告)号：US20120178161A1

    公开(公告)日：2012-07-12

    申请号：US13427548

    申请日：2012-03-22

    申请人： James A. Thomson ,  Tenneille Ludwig

    发明人： James A. Thomson ,  Tenneille Ludwig

    IPC分类号： C12N5/071 ,  C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

    摘要： Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

    摘要翻译： 培养灵长类多能干细胞的以前的方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。

    Method of increasing product expression through solute stress
    80.
    发明申请
    Method of increasing product expression through solute stress 失效
    通过溶质应力增加产品表达的方法

    公开(公告)号：US20050221393A1

    公开(公告)日：2005-10-06

    申请号：US11124746

    申请日：2005-05-09

    申请人： Brian Maiorella ,  Duane Inlow ,  William Howarth

    发明人： Brian Maiorella ,  Duane Inlow ,  William Howarth

    IPC分类号： C12N5/06 ,  C12N5/16 ,  C12P21/04 ,  C12P21/08 ,  G01N33/53 ,  G01N33/567

    CPC分类号： C12N5/163 ,  C12N2500/12 ,  C12N2500/50 ,  C12N2500/90

    摘要： A method of determining the optimal level of product expression and cell growth of animal cell culture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth or growth rate is decreased yet levels of product expression are increased. In a preferred embodiment of the invention is described a method of increasing the yield of monoclonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic products to the culture medium. One- to three-fold increases in antibody yield have been obtained by these methods.

    摘要翻译： 描述了确定动物细胞培养物的产物表达和细胞生长的最佳水平的方法。 该方法通常包括在溶质胁迫条件下培养细胞，即在最佳细胞生长或生长速率降低的条件下，产物表达水平升高。 在本发明的优选实施方案中描述了增加单克隆抗体产量的方法，包括在溶质应激环境中培养杂交瘤细胞。 创造这种环境的一种方法是向培养基中加入无机盐，有机多元醇或代谢产物。 通过这些方法已经获得了抗体产量的一到三倍的增加。