公开(公告)号：US20040137445A1

公开(公告)日：2004-07-15

申请号：US10435185

申请日：2003-05-09

发明人： J. Michael Ramsey

IPC分类号： C12Q001/68 ,  C12M001/34

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/02 ,  G01N30/16 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  Y10S366/01 ,  B01D15/3842

摘要： A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.

摘要翻译： 微芯片装置和方法为各种应用提供流体操作，包括用于微芯片液相色谱的样品注入。 使用标准光刻程序化学湿蚀刻制造微芯片，其中基板和盖板使用直接接合连接。 在基底中形成的通道中进行毛细管电泳。 注射通过电渗透泵送样品通过穿过分离通道的注入通道，然后切换电位以迫使插塞进入分离通道。

公开(公告)号：US20030205469A1

公开(公告)日：2003-11-06

申请号：US10426366

申请日：2003-04-30

发明人： J. Michael Ramsey

IPC分类号： G01N027/26

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/02 ,  G01N30/16 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  Y10S366/01 ,  B01D15/3842

摘要： A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.

公开(公告)号：US20030190756A1

公开(公告)日：2003-10-09

申请号：US10116811

申请日：2002-04-05

发明人： Li-Wei Hsu ,  Su-Chen Chang

IPC分类号： G01N030/02

CPC分类号： G01N30/84 ,  G01N2030/8411 ,  G01N2030/8435

摘要： The present invention discloses a method for ascertaining the quality of herbs by applying the techniques of biochip for detecting the presence of biologically active, desired ingredient(s) in the herbs.

摘要翻译： 本发明公开了一种用于通过应用生物芯片技术检测草药中生物活性的所需成分的存在来确定草药质量的方法。

公开(公告)号：US20030129761A1

公开(公告)日：2003-07-10

申请号：US10327125

申请日：2002-12-24

发明人： Hirotaka Kakita ,  Hiroshi Kamishima

IPC分类号： G01N030/02

CPC分类号： G01N30/84 ,  G01N2030/8435 ,  Y10T436/143333 ,  Y10T436/144444

摘要： In conducting liquid chromatographic analysis of a saccharide mixture containing monosaccharides and oligosaccharides, elution is conducted by using two kinds or more of mobile phases to separate the saccharide mixture into individual constituent saccharides followed by conversion thereof into corresponding derivatives by reaction with reagents and detection of the derivatives as contained in a detector cell. The method of the invention is characterized in that the detector cell is cleaned by washing with a cleaning solvent after completion of the detection of each of the saccharide derivatives. The invention also provides an apparatus for efficiently conducting the above-mentioned inventive method comprising an analytical column, a reactor for converting the separated constituent saccharides into the derivatives, detector cell for containing the derivative, a detector for detecting the derivative contained in the detector cell, a solvent-feed means to introduce a cell-cleaning solvent into the cell and a flow channel-switching means.

摘要翻译： 在对含有单糖和寡糖的糖类混合物进行液相色谱分析时，通过使用两种以上的流动相进行洗脱，将糖类混合物分离为各成分糖类，然后通过与试剂反应转化成相应的衍生物， 衍生物包含在检测器单元中。 本发明的方法的特征在于，检测单元在完成每种糖衍生物的检测后用清洗溶剂进行清洗。 本发明还提供了一种用于有效地进行上述本发明方法的装置，包括分析柱，用于将分离的组成糖转化为衍生物的反应器，用于包含衍生物的检测器单元，用于检测检测器单元中所含的衍生物的检测器 ，将细胞清洗溶剂引入细胞的溶剂进料装置和流路切换装置。

公开(公告)号：US06281019B1

公开(公告)日：2001-08-28

申请号：US09214790

申请日：1999-04-05

申请人： Jürgen Werringloer

发明人： Jürgen Werringloer

IPC分类号： G01N3002

CPC分类号： G01N30/84 ,  G01N2030/8435

摘要： A device for performing a post-column derivatization in connection with liquid chromatography includes a separating column, a detector, an eluent withdrawal unit, connected to an outlet of the separating column, that withdraws eluents from the separating column, a reagent supply unit, arranged between the outlet of the separating column and an inlet of the detector, that supplies reagents to the eluents withdrawn from the separating column and a pump that simultaneously pumps eluents from the eluent withdrawal unit and pumps reagents to the reagent supply unit, wherein the pumping of reagents takes place free of pulsations.

摘要翻译： 用于进行与液相色谱相关的柱后衍生化的装置包括分离塔，检测器，洗脱液提取单元，其连接到分离塔的出口，其从分离塔中抽出洗脱液;试剂供应单元，布置 在分离塔的出口和检测器的入口之间，将试剂供应到从分离塔抽出的洗脱液;以及泵，其同时从洗脱液提取单元抽出洗脱液并将试剂泵送到试剂供应单元，其中泵送 试剂无波动发生。

公开(公告)号：US6033546A

公开(公告)日：2000-03-07

申请号：US153470

申请日：1998-09-15

申请人： J. Michael Ramsey

发明人： J. Michael Ramsey

IPC分类号： G01N33/50 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B29C65/00 ,  B29C65/48 ,  C07B61/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/02 ,  G01N30/16 ,  G01N30/28 ,  G01N30/38 ,  G01N30/60 ,  G01N30/84 ,  G01N37/00 ,  G05D11/02 ,  G01N27/26

CPC分类号： B01L3/5027 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502784 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/16 ,  G01N30/6095 ,  B01F13/0059 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502776 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  G01N30/02 ,  Y10S366/01

摘要： A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.

公开(公告)号：US6001229A

公开(公告)日：1999-12-14

申请号：US283769

申请日：1994-08-01

申请人： J. Michael Ramsey

发明人： J. Michael Ramsey

IPC分类号： G01N33/50 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B29C65/00 ,  B29C65/48 ,  C07B61/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/02 ,  G01N30/16 ,  G01N30/28 ,  G01N30/38 ,  G01N30/60 ,  G01N30/84 ,  G01N37/00 ,  G05D11/02 ,  G01N27/26

CPC分类号： B01L3/5027 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502784 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/16 ,  G01N30/6095 ,  B01F13/0059 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502776 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  G01N30/02 ,  Y10S366/01

摘要： A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.

公开(公告)号：US5783741A

公开(公告)日：1998-07-21

申请号：US791216

申请日：1997-01-31

申请人： Leroy Ellis ,  Ann L. Fincannon

发明人： Leroy Ellis ,  Ann L. Fincannon

IPC分类号： G01N30/72 ,  G01N30/84 ,  H01J49/04 ,  B01D15/08 ,  B01D59/44 ,  G01N30/08

CPC分类号： H01J49/0422 ,  G01N30/84 ,  G01N2030/8435 ,  G01N30/7206

摘要： An isotope ratio monitoring gas chromatograph-mass spectrometer (irm-GC/MS) unit for generating a chromatogram having improved resolution for peaks representative of individual carbon dioxide isotopes measured when a complex GC amenable organic sample (e.g. petroleum hydrocarbons) is combusted in the presence of a metal catalyst. The present irm-GC/MS unit is similar to known irm-GC/MS units of this type except it uses a capillary combustion tube in the furnace which is connected to the capillary column and has approximately the same inside diameter as that of the capillary column (e.g. 0.25 mm I.D.) whereby any "dead volume" attributable to the combustion tube is substantially eliminated.

摘要翻译： 同位素比率监测气相色谱 - 质谱仪（irm-GC / MS）单元，用于产生色谱图，该色谱图具有改善的分辨率，用于当复合GC可使用有机样品（如石油烃）在存在时燃烧时测量出各个二氧化碳同位素的峰值 的金属催化剂。 本irm-GC / MS单元类似于已知的这种类型的GC / MS单元，除了它在熔炉中使用毛细管燃烧管，其连接到毛细管柱并且具有与毛细管的大致相同的内径 柱（例如0.25mm ID），从而基本上消除了归因于燃烧管的任何“死体积”。

公开(公告)号：US5578204A

公开(公告)日：1996-11-26

申请号：US416902

申请日：1995-06-06

申请人： Peter Bartholmes ,  Michael Kaufmann ,  Thomas R. Schwarz

发明人： Peter Bartholmes ,  Michael Kaufmann ,  Thomas R. Schwarz

IPC分类号： G01N30/02 ,  A61K9/08 ,  B01D11/04 ,  B01D15/08 ,  B01D61/24 ,  G01N30/84 ,  G01N30/96

CPC分类号： B01D61/243 ,  B01D15/08 ,  G01N30/96 ,  G01N2030/8435

摘要： The invention relates to an apparatus for recovery and buffer exchange and/or concentration of dissolved macromolecules from a mixture of macromolecules. The apparatus includes a liquid chromatography unit and at least one hollow fiber membrane cartridge connected downstream of the liquid chromatography unit.

摘要翻译： PCT No.PCT / EP93 / 02827 Sec。 371日期：1995年6月6日 102（e）日期1995年6月6日PCT提交1993年10月13日PCT公布。 出版物WO94 / 08684 日期1994年04月28日本发明涉及从大分子混合物中回收和缓冲液交换和/或浓缩溶解的大分子的装置。 该装置包括液相色谱单元和连接在液相色谱单元下游的至少一个中空纤维膜滤芯。

公开(公告)号：US5373725A

公开(公告)日：1994-12-20

申请号：US238578

申请日：1994-05-05

申请人： Albino Sironi ,  Giuseppe Verga

发明人： Albino Sironi ,  Giuseppe Verga

IPC分类号： G01N27/62 ,  B01J19/00 ,  G01N30/68 ,  G01N30/84 ,  G01N31/10 ,  G01N33/28 ,  B01J29/04 ,  G01N1/22

CPC分类号： G01N30/84 ,  B01J19/0093 ,  G01N33/2829 ,  B01J2219/00835 ,  G01N2030/8435 ,  G01N30/68 ,  G01N31/10

摘要： An anlytical equipment and method for the selective detection of individual oxygenated compounds in complex organic mixtures, in particular fuels, comprising, downstream an equipment of chromatographic separation, a cracking reactor, a catalytic hydrogenation microreactor and a FID detector. The cracking reactor is provided with a catalyst under the form of metal elements, made of palladium, inserted inside the capillary tube though which flows the gas under test. Thanks to the present equipment, the analytical process, based on the specific response of oxygenates to the method of flame ionization detection (O-FID), foresees that the cracking reaction takes place at temperatures below 1000 degrees C. and, consequently allows to keep the cracking reactor at stand-by temperatures below 700 degrees C.

摘要翻译： 用于选择性地检测复合有机混合物，特别是燃料中的单个氧化化合物的分析设备和方法，包括在色谱分离设备下游，裂解反应器，催化氢化微反应器和FID检测器。 裂解反应器设置有以钯制成的金属元素形式的催化剂，该催化剂插入毛细管内部，通过其流过被测气体。 由于本设备，基于含氧化合物对火焰离子化检测（O-FID）方法的具体响应的分析过程预见，在低于1000摄氏度的温度下发生裂解反应，因此允许保持 裂解反应器在待机温度低于700摄氏度