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公开(公告)号：US20150027889A1
公开(公告)日：2015-01-29
申请号：US14506265
申请日：2014-10-03
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Alexander Shenderov , Dwayne J. Allen
IPC: B01L3/00 , G01N27/447
CPC classification number: B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
Abstract translation: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。
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公开(公告)号：US20140216932A1
公开(公告)日：2014-08-07
申请号：US14248884
申请日：2014-04-09
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Alexander Shenderov , Zhishan Hua , Arjun Sudarsan
IPC: B01L3/00
CPC classification number: B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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公开(公告)号：US20140014517A1
公开(公告)日：2014-01-16
申请号：US14027941
申请日：2013-09-16
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt
IPC: G01N27/447
CPC classification number: G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
Abstract: Droplet actuator for conducting droplet operations, such as droplet transport and droplet dispensing, is provided. In one embodiment, the droplet actuator may include an electrode that is rotationally but not reflectively symmetrical.
Abstract translation: 提供用于进行液滴操作的液滴致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括旋转但不反射对称的电极。
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84.发明申请公开(公告)号：US20140005066A1
公开(公告)日：2014-01-02
申请号：US13932719
申请日：2013-07-01
Applicant: ADVANCED LIQUID LOGIC INC.
Inventor： Deborah Boles , Lisa Perkins , Allen E. Eckhardt , Jennifer Foley
CPC classification number: C12Q1/703 , C12Q1/686 , C12Q1/701 , C12Q2537/143 , C12Q2561/113 , C12Q2563/107 , C12Q2563/159 , C12Q2565/102
Abstract: The present invention provides a droplet actuator device and methods for multiplexed PCR amplification and detection of target amplicons within a single droplet. The methods of the invention combine quantitative real-time PCR (qPCR) amplification with fluorescence-based sequence specific detection technologies for amplified DNA. In one embodiment, fluorescently-labeled oligonucleotide probes may be used for hybridization-based multiplexed detection of target amplicons. The methods of the invention generally involve combining the necessary reactants to form a PCR-ready droplet and thermal cycling the droplet at temperatures sufficient to result in amplification of one or more target nucleic acids. Fluorescence-based detection techniques may be used for end-point or real-time analysis of DNA amplification. For end-point analysis, the accumulation of a signal, e.g., a fluorescence signal, is measured after the amplification of the target sequence is complete. For real-time analysis, the signal is measured while the amplification reaction is in progress.
Abstract translation: 本发明提供了一种液滴致动器装置和用于在单个液滴内多重PCR扩增和检测靶扩增子的方法。 本发明的方法将定量实时PCR（qPCR）扩增与扩增DNA的基于荧光的序列特异性检测技术相结合。 在一个实施方案中，荧光标记的寡核苷酸探针可用于靶扩增子的杂交多重检测。 本发明的方法通常包括组合必需的反应物以形成PCR准备的液滴并在足以导致一种或多种靶核酸扩增的温度下热循环该液滴。 基于荧光的检测技术可用于DNA扩增的终点或实时分析。 对于终点分析，在靶序列的扩增完成之后测量信号的累积，例如荧光信号。 对于实时分析，在扩增反应进行期间测量信号。
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公开(公告)号：US08592217B2
公开(公告)日：2013-11-26
申请号：US13738259
申请日：2013-01-10
Applicant: Advanced Liquid Logic, Inc.
Inventor： Allen E. Eckhardt
IPC: G01N33/00
CPC classification number: C12Q1/40 , B01L3/502784 , B01L2300/0654 , B01L2300/0816 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07D311/16 , C07D311/18 , C07D311/20 , C07D405/12 , C07D413/12 , C07H15/203 , C07H17/075 , C12Q1/25 , C12Q1/34 , C12Q1/44 , C12Q1/54 , C12Y302/01023 , G01N21/6452 , G01N33/5438 , G01N2333/916 , G01N2333/926 , G01N2333/938 , G01N2800/04
Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.
Abstract translation: 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形酮基衍生物组合，其中所述样品潜在地包含能够切割伞形变体基衍生物的酶，并且其中伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。
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Patent Agency Ranking
公开(公告)号：US20130252262A1
公开(公告)日：2013-09-26
申请号：US13890715
申请日：2013-05-09
Applicant: ADVANCED LIQUID LOGIC INC.
Inventor： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC: G01N27/72
CPC classification number: G01N27/72 , G01N27/745 , G01N33/54326
Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.
Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。
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公开(公告)号：US11865565B2
公开(公告)日：2024-01-09
申请号：US17962989
申请日：2022-10-10
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
CPC classification number: B05B5/0255 , B05B12/082 , G01F23/22 , G01F23/24 , G01F23/26
Abstract: Methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US11465161B2
公开(公告)日：2022-10-11
申请号：US16948238
申请日：2020-09-09
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
Abstract: The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US10731199B2
公开(公告)日：2020-08-04
申请号：US14359177
申请日：2012-11-20
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Carrie A. Graham , Allen E. Eckhardt
IPC: C12Q1/32
Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.
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公开(公告)号：US10139403B2
公开(公告)日：2018-11-27
申请号：US15266693
申请日：2016-09-15
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N1/18 , G01N33/543 , B01F11/00 , B01F13/00 , G01N1/38 , B01F13/08 , B01L7/00 , G01N35/10
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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