公开(公告)号：US20240034973A1

公开(公告)日：2024-02-01

申请号：US18255545

申请日：2021-12-01

申请人： Ushio Denki Kabushiki Kaisha

发明人： Makoto YAMANAKA

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC分类号： C12M23/40 ,  C12M25/16 ,  C12M41/40

摘要： A culture vessel has a culture space therein and may culture cells in the culture space. The culture vessel includes a main flow path extending within a plane, and a secondary flow path that is adjacent to the main flow path in a direction perpendicular to the plane, extends in a direction intersecting the main flow path when viewed from a direction perpendicular to the plane, and partially overlaps the main flow path. The secondary flow path has a groove that is open to and communicates with the main flow path in an area where the secondary flow path overlaps the main flow path, and the culture space is a space within the main flow path, the space being opposed to a contact surface where the secondary flow path is in contact with the main flow path in the area where the secondary flow path overlaps the main flow path.

公开(公告)号：US20240034966A1

公开(公告)日：2024-02-01

申请号：US18265855

申请日：2021-12-02

申请人： Verso Biosense Group Limited

发明人： Joanna Smart ,  Joseph Cefai

IPC分类号： C12M1/107 ,  C12M1/00 ,  C12M1/34 ,  A61B5/00

CPC分类号： C12M21/04 ,  C12M41/14 ,  C12M41/26 ,  C12M41/34 ,  A61B5/4325 ,  A61B5/6875 ,  A61B5/0031

摘要： A cell culture system controller for controlling the environment of a cell culture system, the controller configured to receive intrauterine data and adjust an environmental parameter of a cell culture system environment over a period of time based on the intrauterine data.

公开(公告)号：US20240026262A1

公开(公告)日：2024-01-25

申请号：US18369233

申请日：2023-09-18

申请人： Yeda Research and Development Co. Ltd.

发明人： Yaqub HANNA

IPC分类号： C12M3/00 ,  C12M3/04 ,  C12M1/107 ,  C12M1/34 ,  C12M1/36 ,  C12M1/00

CPC分类号： C12M21/08 ,  C12M27/10 ,  C12M23/36 ,  C12M41/34 ,  C12M41/48 ,  C12M29/20

摘要： Methods and devices for ex-utero mouse embryonic development are provided. Accordingly, there is provided a method of ex-utero culturing a mouse embryo at a zygote stage under conditions that allow developments of the embryo to organogenesis or any developmental stage therein-between. Also provided a fetal incubation system, and methods of using same.

公开(公告)号：US11879135B2

公开(公告)日：2024-01-23

申请号：US16784744

申请日：2020-02-07

申请人： TOKYO ELECTRON LIMITED

发明人： Yusuke Yoda ,  Kunitada Hatabayashi ,  Kenichi Kagawa

IPC分类号： C12N5/074 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12N5/0607 ,  C12M31/00 ,  C12M41/46

摘要： The present disclosure provides a method for determining the undifferentiated state of pluripotent stem cells, comprising: irradiating a test culture medium in which pluripotent stem cells are cultured with wavelength light having a wavelength in the range of 190 nm to 2,500 nm or a partial range thereof; detecting the reflected light, transmitted light or transmitted reflected light thereof to obtain absorbance spectrum data; and analyzing the absorbance at all or part of the measurement wavelengths in the absorbance spectrum data to determine the undifferentiated state of the pluripotent stem cells.

公开(公告)号：US11866687B2

公开(公告)日：2024-01-09

申请号：US16642986

申请日：2018-09-06

申请人： Regents of the University of Minnesota ,  Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.

发明人： Suhasa Kodandaramaiah ,  Elena Taverna ,  Gabriella Shull ,  Wieland Huttner

IPC分类号： G01N35/00 ,  C12M1/36 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26 ,  C12M1/34 ,  G01N35/10 ,  B01L3/02 ,  B01L9/00 ,  C12N15/87 ,  C12N15/89

CPC分类号： C12M41/48 ,  B01L3/0237 ,  B01L9/54 ,  C12M21/08 ,  C12M23/16 ,  C12M23/50 ,  C12M33/04 ,  C12M41/08 ,  C12M41/40 ,  G01N35/00693 ,  G01N35/109 ,  C12N15/87 ,  C12N15/89 ,  G01N35/1002 ,  G01N2035/1039

摘要： Techniques are described for automated microinjection of substances, such as genetic material, into single cells in tissue samples. An example system comprises a robotic manipulator apparatus configured to hold and position a micropipette. Furthermore, the system comprises a microscope camera positioned to observe an injection site. A computing device receives image data from a microscope camera of the system, where the image data represents an image of a tissue sample. The computing device receives, via a user interface, an indication of a line traced by a user on the image of a tissue sample. In response, the computing device controls the robotic manipulator apparatus to move a tip of the micropipette along a path defined by the trajectory line. The pressure controller injects a gas into the micropipette to eject a substance out of the micropipette at one or more points along the path defined by the trajectory line.

公开(公告)号：US11866685B2

公开(公告)日：2024-01-09

申请号：US16939685

申请日：2020-07-27

申请人： UNIVERSITY OF SOUTH CAROLINA

发明人： Ehsan Jabbarzadeh ,  Christopher Wu

IPC分类号： C12M1/34 ,  C12M1/12 ,  C12M1/42 ,  C12M1/00 ,  C12M1/32 ,  C08L33/26 ,  C08L33/10

CPC分类号： C12M41/12 ,  C12M23/20 ,  C12M25/14 ,  C12M35/02 ,  C12M35/04 ,  C08L33/10 ,  C08L33/26 ,  C12M23/12

摘要： Biocompatible, mechanically-dynamic thermoresponsive devices, methods for forming the devices, and exemplary applications for the devices are described. The devices include a series of wells therein and provide for temperature-controlled mechanostimulation of single cells or groups of cells that can be retained in the wells of a device. Mechanostimulation can be single instance of any duration, continuous, or regular or irregular cyclic mechanostimulation of single cells, cell clusters, cell spheroids, or organoids and may be utilized in any of a variety of biomedical applications including, without limitation, cellular engineering, cellular phenotyping, and drug discovery/screening.

公开(公告)号：US20240002898A1

公开(公告)日：2024-01-04

申请号：US18038393

申请日：2021-11-24

申请人： Helmholtz Zentrum Muenchen - Deutsches Forschungszentrum Fuer Gesundheit Und Umwelt (GmbH) ,  Julius-Maximilians-Universitaet Wuerzburg

发明人： Ali DORYAB ,  Otmar SCHMID ,  Andreas SCHROEPPEL ,  Juergen GROLL ,  Mehmet Berat TASKIN

IPC分类号： C12Q1/02 ,  C12M1/12 ,  C12M1/34 ,  C12M1/00

CPC分类号： C12Q1/02 ,  C12M25/04 ,  C12M41/40 ,  C12M29/10 ,  C12M41/46

摘要： In various embodiments a biocompatible composite membrane for in vitro cell culturing comprising a first material, which is non-water soluble and a water soluble second material is provided, wherein the composite membrane comprises a porous scaffold and a filling layer, the scaffold comprising the first material and the filling layer comprising the second material. Further, a method for fabricating the membrane, a bioreactor for use of the membrane in cell-stretch experiments and a corresponding method for investigating cells attached to the biocompatible composite membrane are also provided.

公开(公告)号：US20240002784A1

公开(公告)日：2024-01-04

申请号：US18468223

申请日：2023-09-15

申请人： FUJIFILM Corporation

发明人： Atsushi INADA ,  Kosuke TANIGUCHI ,  Naoto TAKAHASHI

IPC分类号： C12N5/00 ,  C12M1/34

CPC分类号： C12N5/0018 ,  C12M41/42 ,  C12N2500/02

摘要： An object to be achieved by the present invention is to provide a culture method for cells, which makes it possible to suppress oxygen deficiency of cells in a case where the cell is cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, in which a relationship between a stirring power P/V per unit volume, a dissolved oxygen concentration CL0, and a viable cell density VCD satisfies a condition of Expression 1, in cell culture in which the viable cell density is 60×106 cells/mL or more.


0.0059×exp[0.0243×(P/V)]×CL0/VCD≥1.10×10−17   Expression 1:

In the expression, L0 indicates a dissolved oxygen concentration [mol/mL]; VCD indicates a viable cell density [cells/mL]; and P/V indicates a stirring power [W/m3] per unit volume, which is defined by a predetermined expression.

公开(公告)号：US20240002777A1

公开(公告)日：2024-01-04

申请号：US18038831

申请日：2021-12-14

申请人： GLOBAL LIFE SCIENCES SOLUTIONS USA LLC ,  KITE PHARMA, INC.

发明人： PIERRE-YVES CHASSOT ,  OLIVER LINK ,  FEDERICO ZANONI ,  MARK TIMMINS ,  KASHAN ALI SHAIKH ,  MARINE DE LAGENESTE ,  DENNIS CHEROK ,  YANN THOUEMENT ,  YORICK HEIMBERG ,  BERTRAND FOUCAUT ,  JULIEN CAMISANI ,  KENT YOUNG ,  SIMON GARDINER ,  ANTHONY P. SWANDA

IPC分类号： C12M1/00 ,  C12M1/34

CPC分类号： C12M47/02 ,  C12M47/20 ,  C12M41/40

摘要： A kit for magnetic cell isolation includes first stopcock manifold having at least four stopcocks, a separation chamber configured for use with a centrifugal processing chamber of the cell processing device, the separation chamber in fluid communication with the first stopcock manifold, a mixing bag configured for use with a heating/cooling mixing chamber of a cell processing device, the mixing bag in fluid communication with the first stopcock manifold, a second stopcock manifold having at least four stopcocks, the second stopcock manifold in fluid communication with the first stopcock manifold, a magnetic cell isolation holder in fluid communication with the second stopcock manifold, the magnetic cell isolation holder configured for use with a magnetic field generator of a magnetic cell isolation device, and a plurality of cell processing bags in fluid communication with the first and/or second stopcock manifolds.

公开(公告)号：US20240001329A1

公开(公告)日：2024-01-04

申请号：US18112959

申请日：2023-02-22

申请人： ABEC, Inc.

发明人： Eric Rudolph ,  Pete Silverberg ,  Barry Reiss ,  Sarah Murrat ,  Colton Mitchell

IPC分类号： B01J19/18 ,  C12M1/00 ,  C12M1/02 ,  C12M1/34

CPC分类号： B01J19/18 ,  C12M23/28 ,  C12M41/22 ,  C12M41/34 ,  B01J2219/00085 ,  B01J2219/00094 ,  B01J2219/00159 ,  B01J2219/185

摘要： This disclosure relates to reaction container systems providing for headspace-based condensation, coalescing devices, and other features.