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    DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY
    1.
    发明申请
    DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY 有权
    使用成像流程细胞计数检测循环肿瘤细胞

    公开(公告)号：US20120148142A1

    公开(公告)日：2012-06-14

    申请号：US13396333

    申请日：2012-02-14

    申请人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    发明人： William Ortyn ,  David Basiji ,  Luchuan Liang ,  Vidya Venkatachalam ,  Philip Morrissey

    IPC分类号： G06K9/62

    CPC分类号： G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/505 ,  G01N2021/6441

    摘要： Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.

    摘要翻译： 作为细胞分析仪，使用从流动细胞的多模式图像得到的光度和形态测定特征，以确定对应于癌细胞或癌前细胞的标志物是否存在于成像细胞群体中。 成像系统同时获取通过成像系统的视野的每个单元的多个图像。 获取多个不同的图像（即，亮场，暗场和荧光图像）有助于确定不同的形态和形态测量参数。 同时获取多个图像使得能够快速成像相对较大数量的细胞，从而可以检测大量细胞中相对少数量的癌细胞。 首先，将已知的癌细胞成像以使得能够鉴定标记。 然后，可以包含癌细胞的样品被成像以确定标记物是否存在。

    Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging
    5.
    发明授权
    Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging 有权
    用于校正多声道成像的串扰和空间分辨率的方法和装置

    公开(公告)号：US07286719B2

    公开(公告)日：2007-10-23

    申请号：US11419138

    申请日：2006-05-18

    申请人： James K. Riley ,  Keith L. Frost

    发明人： James K. Riley ,  Keith L. Frost

    IPC分类号： G06K9/32 ,  G01N21/00

    CPC分类号： G06K9/00503 ,  G01J3/28 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01N15/147 ,  G01N15/1475 ,  G06K9/00127 ,  G06T5/50 ,  G06T7/32 ,  G06T7/33 ,  G06T2207/10036

    摘要： A multi-channel imaging system generates an ensemble of images for each field of view of an object. Each image in the ensemble is intended to contain information from only one source among a plurality of sources for the object. However, due to crosstalk, at least a portion of the signal from a first source appears in a channel intended for a second source. Because the accuracy of the correction will be degraded if the images in an ensemble are spatially misaligned with respect to one another, the spatial offset between images is determined and a correction is applied to substantially eliminate the offset. Then, a correction to the signals is determined to substantially reduce the contributions to the signal in a channel from the signals in other channels. The signal processing can be employed to process the output signals for each of a plurality of different disclosed imaging systems.

    摘要翻译： 多通道成像系统为对象的每个视野生成图像集合。 集合中的每个图像旨在包含来自对象的多个源中的一个源的信息。 然而，由于串扰，来自第一源的信号的至少一部分出现在用于第二源的通道中。 因为如果一个组合中的图像相对于彼此在空间上不对准，校正的精度将会降低，所以确定图像之间的空间偏移并且应用校正以基本上消除偏移。 然后，确定对信号的校正，以从其它通道中的信号基本上减少对信道中的信号的贡献。 信号处理可用于处理多个不同公开的成像系统中的每一个的输出信号。

    Auto focus for a flow imagin system
    8.
    发明申请
    Auto focus for a flow imagin system 有权
    自动对焦一个流量想象系统

    公开(公告)号：US20050127271A1

    公开(公告)日：2005-06-16

    申请号：US11045675

    申请日：2005-01-28

    申请人： William Ortyn ,  Michael Seo ,  David Basiji ,  Keith Frost ,  David Perry

    发明人： William Ortyn ,  Michael Seo ,  David Basiji ,  Keith Frost ,  David Perry

    IPC分类号： G01N15/10 ,  G01N15/14 ,  G02B27/00 ,  G06T7/20 ,  G01N21/00 ,  G01N15/06 ,  G02B7/04

    CPC分类号： G06T7/20 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/1018 ,  G01N2015/1443 ,  G01N2015/1447 ,  G01N2015/1452 ,  G01N2015/1497 ,  G02B27/0012 ,  G06K9/00134

    摘要： A pair of optical gratings are used to modulate light from an object, and the modulated light from either grating is used to determine the velocity of the object. Each optical grating is offset from a reference focal point by the same distance, one grating being offset in a positive direction, the other in a negative direction. Signals produced in response to the modulated light can be processed to determine a direction in which a primary collection lens should be moved in order to improve a focus of the imaging system on the object. The lens is moved incrementally in the direction so determined, and the process is repeated until an optimal focus is achieved. In a preferred embodiment, the signals are weighted, so that the optical grating disposed closest to the optimal focus position contributes the most to velocity detection.

    摘要翻译： 一对光栅用于调制来自物体的光，并且来自任一光栅的调制光用于确定物体的速度。 每个光栅从参考焦点偏移相同的距离，一个光栅在正方向偏移，另一个光栅在负方向上偏移。 可以处理响应于调制光产生的信号以确定主收集透镜应该移动的方向，以便改善成像系统对物体的焦点。 透镜在如此确定的方向上逐渐移动，并重复该过程，直到达到最佳聚焦。 在优选实施例中，对信号进行加权，使得最靠近最佳聚焦位置布置的光栅最有助于速度检测。

    Multipass cavity for illumination and excitation of moving objects
    10.
    发明申请
    Multipass cavity for illumination and excitation of moving objects 有权
    多光腔用于照明和激发移动物体

    公开(公告)号：US20040080748A1

    公开(公告)日：2004-04-29

    申请号：US10691292

    申请日：2003-10-21

    申请人： Amnis Corporation

    发明人： William E. Ortyn ,  David A. Basiji ,  Keith L. Frost ,  N. Robert McRuer

    IPC分类号： G01N021/00

    CPC分类号： G02B27/126 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/1434 ,  G01N15/147 ,  G01N21/031 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/85 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G02B7/28

    摘要： An illuminiation system for increasing a light signal from an object passing through a reflection cavity. The reflection cavity is disposed between spaced-apart, opposed first and second surfaces disposed on opposite sides of a moving stream of objects. A light collection system is disposed substantially orthogonal to a plane passing through the surfaces and the stream so as to collect light that is scattered from or emitted by the objects as they pass through a field of view disposed between the first and second surfaces. A beam of light from a laser source is directed through the stream of moving objects in a direction nearly orthogonal to the stream (but slightly inclined) and lying in the plane that extends through the surfaces and the stream. Due to the reflection angle and the distance between the stream and the first surface, the point at which the light reflected from the first surface intersects the stream on a second pass is displaced from where it passed though the stream on its initial pass. The light is reflected back and forth between the surfaces a plurality of times, illuminating a different portion of the field of view with each pass until, having ranged over the field of view, the light exits the reflection cavity. The nullrecyclingnull of the light beam in this manner substantially improves the SNR of the detection system by increasing an average illumination intensity experienced by the objects in the stream.

    摘要翻译： 一种用于增加来自穿过反射腔的物体的光信号的照明系统。 反射腔设置在位于物体的移动物流的相对侧上的间隔开的相对的第一和第二表面之间。 光采集系统设置成基本上垂直于穿过表面和流的平面，以便收集当物体经过设置在第一表面和第二表面之间的视野时从物体散射或发射的光。 来自激光源的光束被引导通过几何垂直于流（但稍微倾斜）并且位于延伸穿过表面和流的平面中的移动物体流。 由于反射角度和流与第一表面之间的距离，从第一表面反射的光在第二遍上与流相交的点从其在其初始通过时通过流的位置偏移。 光在表面之间反复反射多次，每次通过照射视场的不同部分，直到在视场范围内，光离开反射腔。 以这种方式，光束的“回收”通过增加物流在物流中经历的平均照明强度，大大提高了检测系统的信噪比。