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公开(公告)号：US11630050B2
公开(公告)日：2023-04-18
申请号：US17382764
申请日：2021-07-22
申请人： Fluidigm Canada Inc.
发明人： Alexandre Loboda
摘要： The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
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公开(公告)号：US11264221B2
公开(公告)日：2022-03-01
申请号：US17253538
申请日：2019-06-18
申请人： Fluidigm Canada Inc. , Paul Corkum
发明人： Paul Corkum , Alexander V. Loboda
IPC分类号： H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/40 , H01J49/16 , H01J49/14
摘要： The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US10745743B2
公开(公告)日：2020-08-18
申请号：US16270904
申请日：2019-02-08
申请人： Fluidigm Canada Inc.
发明人： Olga Ornatsky
IPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6841 , G01N33/58 , H01J49/00
摘要： The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.
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公开(公告)号：US10577648B2
公开(公告)日：2020-03-03
申请号：US14823980
申请日：2015-08-11
申请人： Fluidigm Canada Inc.
发明人： Olga Ornatsky
IPC分类号： G01N33/00 , C12Q1/6825 , C12Q1/6816 , C12Q1/6841 , G01N33/543 , G01N33/58 , G01N33/68 , H01J49/00
摘要： The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.
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公开(公告)号：US20180312794A1
公开(公告)日：2018-11-01
申请号：US15966432
申请日：2018-04-30
申请人： Fluidigm Canada Inc.
发明人： Alexander V. Loboda
CPC分类号： C12M25/00 , G01N1/286 , G01N33/4833 , G01N2001/045 , G01N2001/2886 , H01J49/0027 , H01J49/04
摘要： Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.
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公开(公告)号：US10107734B2
公开(公告)日：2018-10-23
申请号：US15231645
申请日：2016-08-08
申请人： Fluidigm Canada Inc.
发明人： Vladimir Baranov
IPC分类号： G01N15/10 , G01N33/483 , G01N33/569 , G01N1/04 , C12M1/34
摘要： A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.
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公开(公告)号：US09589779B2
公开(公告)日：2017-03-07
申请号：US14783791
申请日：2014-04-17
申请人： Fluidigm Canada Inc.
发明人： Alexandre Loboda
CPC分类号： G01N15/10 , G01N2015/0065 , G01N2015/1006 , H01J49/0463 , H01J49/105 , H01J49/40
摘要： The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
摘要翻译： 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。
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公开(公告)号：US09296838B2
公开(公告)日：2016-03-29
申请号：US14659224
申请日：2015-03-16
申请人： FLUIDIGM CANADA INC.
发明人： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC分类号： G01N33/544 , G01N33/545 , G01N33/24 , C08F8/32 , C08F8/42 , C08F220/36 , G01N33/532 , G01N33/58 , C08F8/14
CPC分类号： C08F8/32 , C08F8/14 , C08F8/42 , C08F220/36 , C08F220/56 , G01N33/532 , G01N33/57426 , G01N33/58 , Y10T436/24
摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
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公开(公告)号：US09012239B2
公开(公告)日：2015-04-21
申请号：US11754340
申请日：2007-05-28
申请人： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
发明人： Mitchell A. Winnik , Mark Nitz , Vladimir Baranov , Xudong Lou
IPC分类号： G01N33/544 , G01N33/545 , G01N33/24 , C08F8/42 , C08F220/36 , G01N33/532 , G01N33/58
CPC分类号： C08F8/32 , C08F8/14 , C08F8/42 , C08F220/36 , C08F220/56 , G01N33/532 , G01N33/57426 , G01N33/58 , Y10T436/24
摘要： Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).
摘要翻译： 提供基于新型金属 - 聚合物共轭物的元件标签用于元素分析，包括ICP-MS。 聚合物主链被官能化以不可逆地结合在用户使用之前选择的金属。 该聚合物进一步官能化以连接允许附着于抗体或其它亲和试剂的接头。 聚合物形式允许附加许多拷贝的给定同位素，其线性地提高灵敏度。 金属 - 聚合物缀合物标签使得能够以两种形式进行多重测定：批量测定，其中样品中的平均生物标志物分布是诊断的，以及单细胞格式以区分复杂样品中的罕见（例如患病）细胞（例如， 血液）。
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公开(公告)号：US20140323339A1
公开(公告)日：2014-10-30
申请号：US14327948
申请日：2014-07-10
申请人： FLUIDIGM CANADA INC.
发明人： Olga Ornatsky
CPC分类号： G01N33/6848 , C12Q1/42 , C12Q1/485 , G01N33/569 , G01N33/58 , G01N33/6842 , G01N2333/91205 , G01N2333/916 , G01N2440/14 , G01N2458/15 , G01N2560/00 , Y10S435/975
摘要： Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes
摘要翻译： 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶enyzmes）方便和准确地分析底物的翻译后修饰
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