INTEGRATED REVERSIBLE CAP
    1.
    发明申请
    INTEGRATED REVERSIBLE CAP 审中-公开
    综合可逆CAP

    公开(公告)号:US20090241239A1

    公开(公告)日:2009-10-01

    申请号:US12054566

    申请日:2008-03-25

    申请人: R. Brent Reynolds

    发明人: R. Brent Reynolds

    IPC分类号: A42B1/06 A42B1/00

    CPC分类号: A42B1/206

    摘要: A reversible hat is converted between at least two visible designs within a substantially integrated unit. For example, the reversible hat can be converted between a blaze orange material and a camouflage material. In one embodiment, a first design is visible on the exterior surface of a first crown. The reversible hat is converted to show a second visible design by folding the exterior surface of a portion of a first crown onto the exterior surface of another portion of the first crown, thereby hiding the first visible design. The folding portion of the first crown is connected to a folding portion of a second crown. Thus, folding a portion of the first crown thereby unfolds a portion of the second crown. Folding the exterior surface of a portion of a second crown onto the exterior surface of another portion of the second crown reveals the first visible design again.

    摘要翻译: 可逆帽子在基本上集成的单元内的至少两个可见设计之间转换。 例如,可逆帽可以在火焰橙色材料和伪装材料之间转换。 在一个实施例中,在第一冠的外表面上可见第一设计。 通过将第一冠部的一部分的外表面折叠到第一冠部的另一部分的外表面上,可逆的帽子被转换为显示第二可见设计,从而隐藏第一可见设计。 第一冠部的折叠部分连接到第二冠部的折叠部分。 因此,折叠第一冠的一部分从而展开第二冠的一部分。 将第二冠部的一部分的外表面折叠到第二冠部的另一部分的外表面上再次显示第一可见设计。

    Multipotent neural stem cell cDNA libraries
    2.
    发明授权
    Multipotent neural stem cell cDNA libraries 失效
    多能神经干细胞cDNA文库

    公开(公告)号:US07105342B2

    公开(公告)日:2006-09-12

    申请号:US09925911

    申请日:2001-08-09

    IPC分类号: C12N15/63 C12N15/12

    摘要: cDNA libraries may be obtained from neural cell cultures produced by using growth factors to induce the proliferation of multipotent neural stem cells. The libraries may be obtained from both cultured normal and dysfunctional neural cells and from neural cell cultures at various stages of development. This information allows for the identification of the sequence of gene expression during neural development and can be used to reveal the effects of biological agents on gene expression in neural cells. Additionally, nucleic acids derived from dysfunctional tissue can be compared with that of normal tissue to identify genetic material which may be the cause of the dysfunction. This information could then be used in the design of therapies to treat the neurological disorder. A further use of the technology would be in the diagnosis of genetic disorders or for use in identifying neural cells at a particular stage in development.

    摘要翻译: cDNA文库可以从使用生长因子产生的神经细胞培养物获得以诱导多能神经干细胞的增殖。 文库可以从培养的正常和功能障碍的神经细胞和来自神经细胞培养物的不同发展阶段获得。 该信息允许在神经发育期间鉴定基因表达序列,并且可以用于揭示生物剂对神经细胞中的基因表达的影响。 另外,来自功能障碍组织的核酸可以与正常组织的核酸进行比较,以鉴定可能是功能障碍的原因的遗传物质。 然后可以将该信息用于设计治疗神经障碍的治疗。 该技术的进一步使用将在于遗传疾病的诊断或用于在发育的特定阶段鉴定神经细胞。

    Neural colony forming assay
    9.
    发明申请
    Neural colony forming assay 有权
    神经集落形成试验

    公开(公告)号:US20050112546A1

    公开(公告)日:2005-05-26

    申请号:US10938579

    申请日:2004-09-13

    CPC分类号: G01N33/5073 G01N33/56966

    摘要: A neural colony forming cell (NCFC) assay is described. The assay allows one to distinguish neural stem cells from neural progenitor cells. In one embodiment, the present invention provides a method for identifying neural stem cells or neural progenitor cells comprising: (a) suspending neural cells in a semi-solid medium which supports the growth of neural cells; (b) plating the cells in the semi-solid medium at a density that allows for the production of colonies; (c) culturing the plated cells until size differences can be discerned between the colonies; and (d) estimating colony size wherein the larger colonies are likely produced by neural stem cells and wherein the small colonies are likely produced by neural progenitor cells In alternate embodiments, NSC can be distinguished from neural progenitor cells by determining the morphology or antigen expression of the colonies.

    摘要翻译: 描述了神经集落形成细胞(NCFC)测定。 该测定允许区分神经干细胞与神经祖细胞。 在一个实施方案中,本发明提供了用于鉴定神经干细胞或神经祖细胞的方法,包括:(a)将神经细胞悬浮于支持神经细胞生长的半固体培养基中; (b)以允许生产菌落的密度在半固体培养基中电镀细胞; (c)培养电镀细胞,直到在菌落之间可以看出尺寸差异; 和(d)估计集落大小,其中较大的菌落可能由神经干细胞产生,并且其中小菌落可能由神经祖细胞产生。在替代实施方案中,NSC可以通过确定神经祖细胞的形态或抗原表达来区分 殖民地