Lyophilization of bacteria
    4.
    发明授权
    Lyophilization of bacteria 失效
    细菌冻干

    公开(公告)号:US4205132A

    公开(公告)日:1980-05-27

    申请号:US925034

    申请日:1978-07-17

    摘要: Storage stable, lyophilized, acid producing bacteria, such as lactic acid bacteria, are prepared by lyophilizing the bacteria in the presence of at least 5% by weight of the bacteria of a basic organic or inorganic buffering agent, preferably an alkali metal salt of glycerophosphate, to provide lyophilized bacteria containing less than 5% by weight water, and sealing the lyophilized bacteria in a container while in a vacuum or surrounded by a gaseous atmosphere substantially free of oxygen, preferably argon which is essentially free of oxygen.

    摘要翻译: 在至少5重量%的碱性有机或无机缓冲剂的细菌,优选甘油磷酸的碱金属盐的存在下,将细菌冻干,制备储存稳定的,冻干的酸产细菌如乳酸菌 提供含有小于5重量%水的冻干细菌,并且在真空中将冻干细菌密封在容器中,或者由基本上不含氧的气体气氛(优选基本上不含氧的氩气)包围。

    Streptococcus lactis plasmid encoding for mucoidness
    6.
    发明授权
    Streptococcus lactis plasmid encoding for mucoidness 失效
    乳酸链球菌质粒编码粘液性

    公开(公告)号:US5672477A

    公开(公告)日:1997-09-30

    申请号:US632642

    申请日:1996-04-15

    摘要: A lactose-fermenting (Lac.sup.+), mucoid (Muc.sup.+) Streptococcus lactis transconjugant designated ML-3/2.230 was obtained by conjugative co-transfer of Lac.sup.+ Muc.sup.+ phenotypes from Lac.sup.+ Muc.sup.+ Streptococcus cremoris MS to lactose-negative (Lac.sup.-), non-mucoid S. lactis ML-3/2.2. Curing studies on the transconjugant ML-3/2.230 showed that Muc.sup.+ phenotype in the strain was encoded on two plasmids, pSRQ2203 and pSRQ2204. DNA hybridization experiments also indicated the involvement of two plasmids in the expression of the mucoid phenotype. Plasmid pSRQ2203 also contained genetic information for lactose utilization (Lac.sup.+).

    摘要翻译: 通过从Lac + Muc + Crestococcus cremoris MS的Lac + Muc +表型与乳糖阴性(Lac-),非粘液样的共轭共转移获得了称为ML-3 / 2.230的乳糖发酵(Lac +),粘液样(Muc +)乳酸链球菌转运结合物 乳酸乳球菌ML-3 / 2.2。 转录结合ML-3 / 2.230的固化研究表明,菌株中的Muc +表型被编码在两个质粒pSRQ2203和pSRQ2204上。 DNA杂交实验也表明两种质粒参与了粘液表型的表达。 质粒pSRQ2203还含有乳糖利用的遗传信息(Lac +)。

    Stabilizing agents for frozen lactic acid producing bacteria compositions
    9.
    发明授权
    Stabilizing agents for frozen lactic acid producing bacteria compositions 失效
    用于冷冻乳酸生成细菌组合物的稳定剂

    公开(公告)号:US3975545A

    公开(公告)日:1976-08-17

    申请号:US586943

    申请日:1975-06-16

    摘要: Frozen compositions of lactic acid producing bacteria which incorporate an alkali metal salt of glycerophosphoric acid are described. These salts reduce damage to the bacteria upon freezing and storage which with certain bacteria is evidenced by enhanced lactic acid forming activity upon thawing and use of the compositions as fermented dairy product starter cultures.

    摘要翻译: 描述了掺入甘油磷酸的碱金属盐的乳酸生产细菌的冷冻组合物。 这些盐在冷冻和储存时减少对细菌的损害,这些细菌通过在解冻时增加乳酸形成活性和使用组合物作为发酵乳制品发酵剂培养物来证明。