公开(公告)号：US07914981B2

公开(公告)日：2011-03-29

申请号：US10854678

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US20070054306A1

公开(公告)日：2007-03-08

申请号：US11590384

申请日：2006-10-31

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US20070048783A1

公开(公告)日：2007-03-01

申请号：US11590056

申请日：2006-10-31

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

摘要翻译： 本发明涉及鉴定靶核苷酸序列的方法。 该方法包括在连接酶检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸 序列。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

公开(公告)号：US20050233320A1

公开(公告)日：2005-10-20

申请号：US09963920

申请日：2001-09-26

申请人： Francis Barany ,  George Barany ,  Robert Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： B01J19/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  C12M1/34

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。 连接阶段之前可以进行扩增过程。 本发明还涉及一种用于实施该方法的试剂盒，在固体支持物上形成阵列的方法，以及载体本身。

公开(公告)号：US5656707A

公开(公告)日：1997-08-12

申请号：US491474

申请日：1995-06-16

申请人： Maria Kempe ,  George Barany

发明人： Maria Kempe ,  George Barany

IPC分类号： C08F20/28 ,  C08F22/10 ,  C08F290/06 ,  C08F18/00

CPC分类号： C07K1/042 ,  C08F20/28 ,  C08F22/105 ,  C08F290/062

摘要： A polymer useful as a solid support for a variety of applications is provided. The polymer is prepared from a composition comprising at least one type of olefinic monomer and a multifunctional oxyethylene-containing meth(acrylate) crosslinker of the following formula (I): ##STR1## wherein: (a) R.sup.1, R.sup.2, and R.sup.3 are each independently hydrogen or a methyl group;(b) R.sup.4 is hydrogen or an organic group or substituent that can interact in the polymerization and/or crosslinking process or is nonreactive under the conditions of the polymerization and/or crosslinking process; and(c) each of l, m, and n is no greater than about 100.

摘要翻译： 提供了可用作各种应用的固体支持物的聚合物。 聚合物由包含至少一种类型的烯属单体和下式（I）的多官能含氧乙烯的（丙烯酸酯）交联剂的组合物制备：其中：（a）R1，R2和 R3各自独立地为氢或甲基; （b）R4是氢或可在聚合和/或交联过程中相互作用的或在聚合和/或交联过程的条件下是非反应性的有机基团或取代基; 和（c）l，m和n中的每一个不大于约100。

公开(公告)号：US08597890B2

公开(公告)日：2013-12-03

申请号：US13523252

申请日：2012-06-14

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

摘要翻译： 本发明涉及鉴定靶核苷酸序列的方法。 该方法包括在连接检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸 序列。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

公开(公告)号：US07888009B2

公开(公告)日：2011-02-15

申请号：US10854482

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US20100173802A1

公开(公告)日：2010-07-08

申请号：US12725230

申请日：2010-03-16

申请人： Francis BARANY ,  George BARANY ,  Robert P. HAMMER ,  Maria KEMPE ,  Herman BLOK ,  Monib ZIRVI

发明人： Francis BARANY ,  George BARANY ,  Robert P. HAMMER ,  Maria KEMPE ,  Herman BLOK ,  Monib ZIRVI

IPC分类号： C40B40/06 ,  C40B60/12

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07312039B2

公开(公告)日：2007-12-25

申请号：US11590056

申请日：2006-10-31

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US07083917B2

公开(公告)日：2006-08-01

申请号：US09963920

申请日：2001-09-26

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C07H21/02 ,  G01N33/543

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.