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公开(公告)号：US20050029440A1
公开(公告)日：2005-02-10
申请号：US10498212
申请日：2002-12-11
IPC分类号： G01N27/62 , B01L3/00 , B01L3/02 , G01N1/28 , G01N1/34 , G01N1/40 , G01N27/447 , G01N33/483 , G01N33/68 , G01N35/00 , G01N35/02 , G01N35/10 , H01J49/00 , B01D59/44
CPC分类号： B01L3/0268 , B01J2219/00315 , B01J2219/00317 , B01J2219/0036 , B01J2219/00364 , B01J2219/00378 , B01J2219/00452 , B01J2219/005 , B01J2219/00585 , B01J2219/00599 , B01J2219/00704 , B01J2219/00725 , B01J2219/00731 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N35/028 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053
摘要： A device and method useable for fast processing and depositing of biomolecules. The method includes the following steps: receiving a first set of analytes to be analysed; passing in an array format, analytes through extraction means where said biomolecules adhere to solid phase means; washing said solid phase means with a washing liquid, leaving only said biomolecules; performing elution by passing portions of organic solvent through each said solid phase, forming eluates constituting a second set of analytes containing said biomolecules, as a result of said elution; dispensing, in an array format, said second set of analytes using a micro dispensing array; controlling said array to dispense precise amounts of each one of said second set of analytes on target areas of a target plate.
摘要翻译： 可用于快速处理和沉积生物分子的装置和方法。 该方法包括以下步骤：接收待分析的第一组分析物; 通过提取方式传递数组格式的分析物，其中所述生物分子粘附于固相手段; 用洗涤液清洗所述固相装置，仅留下所述生物分子; 通过使有机溶剂通过每个所述固相进行洗脱，由于所述洗脱，形成构成含有所述生物分子的第二组分析物的洗脱液; 使用微量分配阵列以阵列形式分配所述第二组分析物; 控制所述阵列以在目标板的目标区域上分配所述第二组分析物中的每一个的精确量。
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公开(公告)号：US20050070010A1
公开(公告)日：2005-03-31
申请号：US10497996
申请日：2002-12-11
IPC分类号： G01N27/62 , B01L3/00 , B01L3/02 , G01N1/10 , G01N1/34 , G01N1/40 , G01N27/447 , G01N27/64 , G01N30/00 , G01N30/08 , G01N30/46 , G01N30/60 , G01N30/88 , G01N35/00 , G01N35/02 , G01N35/10 , C12M1/34
CPC分类号： B01L3/0268 , B01J2219/00315 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00452 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00691 , B01J2219/00704 , B01J2219/00725 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N35/028 , G01N35/1065 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053
摘要： A processing module for extracting certain biomolecules from a solution, comprising an extraction unit having at least one elongated channel (101) each of said at least sine channel have un inlet (102) and an outlet (103) and being provided with adhering units (201), each said unit being provided with adhesive means, having affinity for said certain biomolecules, said extraction unit further comprises docking means (205) having an inlet array and an outlet array, that enables the extractor to be docked to and undocked from other devices having corresponding docking means, such that said solution or another fluid can be made to flow from said other devices, entering the inlet array, to flow through the at least one channels(101) and to leave the extraction device via the outlet array.
摘要翻译： 一种用于从溶液中提取某些生物分子的处理模块，包括具有至少一个细长通道（101）的提取单元，每个所述至少正弦通道具有非入口（102）和出口（103），并且设有粘附单元 每个所述单元设置有对所述某些生物分子具有亲和性的粘合剂装置，所述提取单元还包括具有入口阵列和出口阵列的对接装置（205），其使得提取器能够对接到其它物体上 具有相应对接装置的装置，使得所述溶液或另一种流体可以被制成从所述其它装置流出，进入入口阵列，以流过所述至少一个通道（101）并且经由所述出口阵列离开所述提取装置。
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公开(公告)号：US20050042769A1
公开(公告)日：2005-02-24
申请号：US10497992
申请日：2002-12-11
IPC分类号： G01N27/62 , B01L3/00 , B01L3/02 , G01N1/00 , G01N1/34 , G01N1/40 , G01N27/447 , G01N30/46 , G01N30/72 , G01N30/84 , G01N30/88 , G01N33/68 , G01N35/00 , G01N35/02 , G01N35/10 , G01N1/10
CPC分类号： B01L3/0268 , B01J2219/00315 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00452 , B01J2219/005 , B01J2219/00576 , B01J2219/00581 , B01J2219/00585 , B01J2219/00596 , B01J2219/00691 , B01J2219/00704 , B01J2219/00722 , B01J2219/00725 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N35/028 , G01N35/1065 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053 , Y10T436/2575
摘要： The invention relates to a method and a machine for biomolecule handling using an array dispenser. More particularly, the invention relates to a method and a machine in which one or two separation procedures are performed in parallel channels and the separated biomolecules are deposited on a two-dimensional target plate for analysis e.g. in a MALDI TOF MS device (Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry). A method of sample handling comprises the following steps: supplying (101) a number of samples to a multichannel separation device having an equal number of separation channels; separating (102) the samples in parallel in said number of separation channels; supplying said channels to an array dispenser after separation; dispensing (103) said channels in parallel on to a target plate maintaining the separation of the multichannel separation device.
摘要翻译： 本发明涉及一种使用阵列分配器进行生物分子处理的方法和机器。 更具体地说，本发明涉及一种方法和机器，其中在平行通道中进行一个或两个分离程序，并将分离的生物分子沉积在二维靶板上用于分析。 在MALDI TOF MS装置（Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry）中。 一种样品处理方法包括以下步骤：向具有相等数目的分离通道的多通道分离装置提供（101）多个样品; 在所述数量的分离通道中并行分离（102）样品; 分离后将所述通道供给阵列分配器; 将所述通道平行地分配（103）到目标板上，保持多通道分离装置的分离。
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公开(公告)号：US20050031496A1
公开(公告)日：2005-02-10
申请号：US10498043
申请日：2002-12-11
IPC分类号： G01N27/64 , B01L3/00 , B01L3/02 , C40B20/02 , C40B60/10 , G01N1/00 , G01N1/34 , G01N1/40 , G01N27/447 , G01N27/62 , G01N33/483 , G01N33/68 , G01N35/00 , G01N35/02 , G01N35/10 , H01J49/04 , G01N24/00
CPC分类号： G01N35/028 , B01J2219/00315 , B01J2219/00317 , B01J2219/0036 , B01J2219/00585 , B01J2219/00599 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053 , H01J49/0418 , Y10T436/24
摘要： A plate suitable for use with mass spectrometers comprising a number of target spots arranged in a surface portion of said plate making it possible to deposit small amounts of fluid at said target spots without the fluid escaping or getting mixed with fluid deposited at another target surface of the same plate. The target surfaces being arranged in a surface portion of said plate so that a base material of said plate constitutes the walls of receptacles, characterised in that the shape, size, temperature and possible agents of said receptacle facilitate evaporation of a solution in which sample molecules are suspended. Embodiments include different types of matrix and enzymes arranged at said spots, and methods for enhancing MALDI analysis efficiency.
摘要翻译： 适合与质谱仪一起使用的板，其包括布置在所述板的表面部分中的多个靶点，使得可以在所述目标点处沉积少量流体，而不会使流体逸出或与沉积在另一目标表面上的流体混合 同一盘。 目标表面布置在所述板的表面部分中，使得所述板的基材构成容器的壁，其特征在于，所述容器的形状，尺寸，温度和可能的试剂有助于蒸发溶液，其中样品分子 被暂停。 实施例包括布置在所述斑点处的不同类型的基质和酶，以及用于增强MALDI分析效率的方法。
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公开(公告)号：US20100203565A1
公开(公告)日：2010-08-12
申请号：US11909235
申请日：2006-03-20
CPC分类号： G01N33/6848 , G01N33/6893 , G01N2800/122
摘要： Methods for producing and using protein/peptide fingerprints, derived from elastin degraded by the enzyme human Neutrophil Elastase (HNE), allowing identification and investigation of disease-associated proteins/peptides that can be linked to specific drug targets, or to specific drug target combinations. The methods are particularly useful for studies relating to Chronic Obstructive Pulmonary Disease (COPD).
摘要翻译： 用于生产和使用蛋白质/指纹图谱的衍生自由人嗜中性粒细胞弹性蛋白酶（HNE）降解的弹性蛋白的蛋白质/指纹图谱，允许识别和研究可与特定药物靶标相关的疾病相关蛋白质/肽或特异性药物靶组合 。 这些方法对于与慢性阻塞性肺疾病（COPD）有关的研究特别有用。
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公开(公告)号：US20080227117A1
公开(公告)日：2008-09-18
申请号：US11912728
申请日：2006-04-27
CPC分类号： C07K16/18 , G01N33/6893
摘要： The present invention relates to the identification of biomarkers for the disease condition COPD. The uses of such biomarkers in diagnosis and therapy and a novel method for their identification is are also described.
摘要翻译： 本发明涉及鉴定疾病状况COPD的生物标志物。 还描述了这样的生物标志物在诊断和治疗中的用途以及用于鉴定的新方法。
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7.发明申请公开(公告)号：US20060292631A1
公开(公告)日：2006-12-28
申请号：US10572872
申请日：2004-09-24
CPC分类号： G01N33/6884 , G01N2800/122 , Y10T436/24
摘要： Methods for producing and using protein/peptide fingerprints, allowing identification and investigation of disease-associated proteins/peptides that can be linked to specific drug targets, or to specific drug target combinations. The methods are particularly useful for studies relating to Chronic Obstructive Pulmonary Disease (COPD), especially for the enzyme MMP12.
摘要翻译： 用于生产和使用蛋白质/肽指纹图谱的方法，允许识别和调查可与特定药物靶标相关的疾病相关蛋白质/肽或特定药物靶组合。 该方法对于与慢性阻塞性肺疾病（COPD）有关的研究特别适用于MMP12酶。
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公开(公告)号：US20050048568A1
公开(公告)日：2005-03-03
申请号：US10498072
申请日：2002-12-11
IPC分类号： G01N33/68 , B01L3/00 , B01L3/02 , G01N1/34 , G01N1/40 , G01N27/447 , G01N33/48 , G01N35/00 , G01N35/02 , G01N35/10 , G01N37/00 , G01N33/53 , C12M1/34
CPC分类号： B01L3/0268 , B01J2219/00378 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00725 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N35/028 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053
摘要： A method of selecting and identifying bio-molecules present in a bio-sample is disclosed. The method comprises the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the case of detection of said bio-molecules; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media. Detecting means are then used to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step. A device (1) for carrying out the method is also disclosed as is a protein chip library produced by the device or method.
摘要翻译： 公开了选择和鉴定存在于生物样品中的生物分子的方法。 该方法包括以下步骤：获得生物样品; 扩增（2）存在于生物样品中的生物分子以改善所述生物分子的检测情况; 分离所述扩增的生物样品中的生物分子; 将扩增的生物分子沉积（3）到合适的介质上。 然后使用检测手段来鉴定或检测所述扩增和分离的样品中生物分子的存在，其中所述扩增步骤在与所述沉积步骤紧密物理接近的地方发生。 还公开了用于实施该方法的装置（1），由装置或方法产生的蛋白质芯片库也是如此。
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公开(公告)号：US20050047962A1
公开(公告)日：2005-03-03
申请号：US10498073
申请日：2002-12-11
IPC分类号： G01N27/64 , B01L3/00 , B01L3/02 , G01N1/00 , G01N1/10 , G01N1/34 , G01N1/40 , G01N27/447 , G01N35/00 , G01N35/02 , G01N35/10 , G01N37/00 , H02N2/00 , H01L41/04
CPC分类号： B01L3/0268 , B01J2219/00315 , B01J2219/00317 , B01J2219/00364 , B01J2219/00378 , B01J2219/00452 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00691 , B01J2219/00704 , B01J2219/00725 , B01L3/0241 , B01L3/5085 , B01L2200/0636 , B01L2200/0668 , B01L2300/0829 , B01L2300/0864 , B01L2300/18 , B01L2400/0439 , B01L2400/0481 , B01L2400/0688 , G01N1/405 , G01N27/44756 , G01N27/44769 , G01N35/028 , G01N35/1065 , G01N2035/00564 , G01N2035/1034 , G01N2035/1053
摘要： A dispensing device for use in chemical analysis comprising at least two dispenser nozzles, a chamber having at least two inlets, a membrane entity constituting part of defining elements of said chamber, said membrane entity comprising at least one flexible membrane, and an actuation element, such that liquids brought to flow through said inlets into said chamber can be pressurised by actuating the membrane entity by providing a pulse to said actuation element, and thereby dispensing an amount of liquid through each of said at least two nozzles. Embodiments include devices comprising integrated free flow electrophoresis separation means.
摘要翻译： 一种用于化学分析的分配装置，包括至少两个分配器喷嘴，具有至少两个入口的室，构成所述室的限定元件的一部分的膜实体，所述膜实体包括至少一个柔性膜和致动元件， 使得通过所述入口流入所述室的液体可以通过通过向所述致动元件提供脉冲致动膜实体来加压，从而通过每个所述至少两个喷嘴分配一定量的液体。 实施例包括包括集成自由流动电泳分离装置的装置。
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10.发明申请Device and method for analysis of samples using a combined sample treatment and sample carrier device 审中-公开使用组合样品处理和样品载体装置分析样品的装置和方法公开(公告)号：US20060252047A1
公开(公告)日：2006-11-09
申请号：US10564938
申请日：2004-07-14
CPC分类号： H01J49/0418 , B01L3/0241 , B01L3/50255 , B01L3/50857 , B01L3/5088 , B01L2300/0819 , G01N33/54366
摘要： The invention relates to devices and methods for chemical analysis, specifically to devices for extracting molecules, e.g. biomolecules such as peptides, and/or proteins, from a mixture of molecules in a solution and performing sample treatment on the extracted molecules before presenting them to an analysis instrument. The method for analysis of samples uses a combined sample treatment and sample carrier device. Said device comprises a plate with inlets at one side connected to respective compartments situated at respective array positions for receiving samples to be treated and analysed, each compartment being in communication with an outlet enabling fluid flow through the plate. The method comprises the steps of supplying an external container with a sample; optionally, subjecting the sample to a first treatment in the external container; transferring the sample to the combined sample treatment and sample carrier device; and subjecting the sample to a second treatment exploiting fluid flow through the device, wherein a medium is trapped in the device.
摘要翻译： 本发明涉及用于化学分析的装置和方法，具体涉及用于提取分子的装置，例如， 生物分子如肽和/或蛋白质，从溶液中的分子混合物中，并在将其提供给分析仪器之前对提取的分子进行样品处理。 样品分析方法采用组合样品处理和样品载体装置。 所述装置包括板，其一侧的入口连接到位于相应阵列位置处的相应隔室，用于接收待处理和分析的样品，每个室与允许流体流过板的出口连通。 该方法包括向外部容器提供样品的步骤; 任选地，使样品在外部容器中进行第一次处理; 将样品转移到组合的样品处理和样品载体装置; 并且对样品进行第二处理，利用流体流过该装置，其中介质被捕获在装置中。
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