公开(公告)号：US20120258529A1

公开(公告)日：2012-10-11

申请号：US13272338

申请日：2011-10-13

申请人： Hee-kyun LIM ,  Joon-ho KIM ,  Myung-gwon LEE ,  Je-kyun PARK

发明人： Hee-kyun LIM ,  Joon-ho KIM ,  Myung-gwon LEE ,  Je-kyun PARK

IPC分类号： C12N5/078 ,  C12M1/12

CPC分类号： B01L3/502746 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0655 ,  B01L2400/086

摘要： An apparatus for separating target molecules includes a plurality of protruding portions on a first sidewall of a fluid channel to control a flow of a fluid containing the target molecules, and a fluid channel portion having a variable height for separating the target molecules depending on sizes of the target molecules.

摘要翻译： 用于分离目标分子的装置包括在流体通道的第一侧壁上的多个突出部分，以控制含有目标分子的流体的流动，以及具有可变高度的流体通道部分，用于根据 目标分子。

公开(公告)号：US07090977B2

公开(公告)日：2006-08-15

申请号：US10282251

申请日：2002-10-29

申请人： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

发明人： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/173

摘要： Disclosed is a method for detecting nucleic acid hybridization by using intercalator binding to hybridized nucleic acid, wherein oxidation-reduction of transition metallic complex is induced to cause electrochemiluminescence, thereby providing a method for detecting nucleic acid hybridization without a special labeling.

摘要翻译： 公开了通过使用嵌入剂与杂交核酸结合来检测核酸杂交的方法，其中诱导过渡金属络合物的氧化还原引起电化学发光，从而提供用于检测核酸杂交而不进行特殊标记的方法。

公开(公告)号：US5705045A

公开(公告)日：1998-01-06

申请号：US703537

申请日：1996-08-27

申请人： Je-Kyun Park ,  Kang Shin Lee

发明人： Je-Kyun Park ,  Kang Shin Lee

IPC分类号： C12Q1/00 ,  C12Q1/52 ,  G01N27/26

CPC分类号： C12Q1/52 ,  C12Q1/005 ,  Y10S435/817

摘要： Multi-biosensor in which only one enzyme is used for handy and simultaneous measurement of GPT and GOT activities, and a method for fabricating the multi-biosensor, are disclosed, the biosensor including an insulating substrate; a reference electrode formed on the insulating substrate; first and second working electrodes formed on one side of the insulating substrate having the reference electrode formed thereon; third and fourth working electrodes formed on the other side of the insulating substrate having the reference electrode formed thereon opposite to the first and second working electrodes centered on the reference electrode; first, second, third and fourth carbon paste layers formed on the first, second, third and fourth working electrodes respectively; first and second enzyme/polymer carbon paste layers formed on the first and second carbon paste layers respectively; first and second non-enzyme/polymer carbon paste layers formed on the third and fourth carbon paste layers respectively; an amino acid/polymer paste layer for making an enzymatic reaction with GPT formed extended to the first enzyme/polymer carbon paste layer and the first non-enzyme/polymer carbon paste layer; and, an amino acid/polymer paste layer for making an enzymatic reaction with GOT formed extended to the second enzyme/polymer carbon paste layer and the second non-enzyme/polymer carbon paste layer.

摘要翻译： 公开了其中仅使用一种酶进行GPT和GOT活性的方便和同时测量的多生物传感器，以及制造多生物传感器的方法，所述生物传感器包括绝缘基板; 形成在所述绝缘基板上的参考电极; 形成在其上形成有参考电极的绝缘基板的一侧上的第一和第二工作电极; 第三工作电极和第四工作电极，形成在绝缘衬底的另一侧上，其具有形成在其上的参考电极，与第一工作电极和第二工作电极相对，以参考电极为中心; 分别形成在第一，第二，第三和第四工作电极上的第一，第二，第三和第四碳糊层; 分别形成在第一和第二碳糊层上的第一和第二酶/聚合物碳糊层; 分别在第三和第四碳糊层上形成的第一和第二非酶/聚合物碳糊层; 用于与形成的GPT进行酶反应的氨基酸/聚合物糊层延伸到第一酶/聚合物碳糊层和第一非酶/聚合物碳糊层; 并且用于与GOT形成酶反应的氨基酸/聚合物糊层延伸到第二酶/聚合物碳糊层和第二非酶/聚合物碳糊层。

公开(公告)号：US08962258B2

公开(公告)日：2015-02-24

申请号：US13060402

申请日：2008-08-29

申请人： Je-Kyun Park ,  MinSeok Kim ,  Eun Sook Lee ,  Sun-Young Kong ,  Solm Kwon

发明人： Je-Kyun Park ,  MinSeok Kim ,  Eun Sook Lee ,  Sun-Young Kong ,  Solm Kwon

IPC分类号： G01N33/53 ,  G01N1/31 ,  B01L3/00 ,  G01N1/28 ,  G01N1/30

CPC分类号： G01N1/312 ,  B01L3/502 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/0883 ,  G01N1/28 ,  G01N1/2806 ,  G01N1/2813 ,  G01N1/30 ,  G01N33/5302 ,  Y10S435/96 ,  Y10S435/973

摘要： Provided are a multiple immunoassay apparatus on a chip in which a structure comprising multiple microfluidic channels is associated with a tissue sample, which allows immunohistochemical reactions to be conducted therein, to examine various markers specific for certain diseases, and a method for performing multiple immunoassays using the same. The multiple immunoassay apparatus comprises: at least one antibody-introducing unit through which at least one antibody is introduced into the apparatus; at least one reaction unit in which the antibody reacts with a sample in an immunohistochemical pattern; and at least one fluid outlet through which a fluid including the antibody is discharged outside the apparatus.

摘要翻译： 提供了一种芯片上的多重免疫测定装置，其中包含多个微流体通道的结构与允许在其中进行免疫组织化学反应的组织样品相关联，以检查对某些疾病特异性的各种标志物，以及使用 一样。 所述多重免疫测定仪器包括：至少一个抗体导入单元，通过所述至少一个抗体引入单元将至少一种抗体引入所述装置中; 至少一个反应单元，其中所述抗体以免疫组织化学模式与样品反应; 以及至少一个流体出口，包含抗体的流体通过该流体出口排出设备外部。

公开(公告)号：US20060292595A1

公开(公告)日：2006-12-28

申请号：US11376296

申请日：2006-03-16

申请人： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

发明人： Kyu-Sik Yun ,  Jeong-Gun Lee ,  Je-Kyun Park ,  Su-Hyeon Kim ,  Sang-Eun Lee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2563/173

摘要： Disclosed is a method for detecting nucleic acid hybridization by using intercalator binding to hybridized nucleic acid, wherein oxidation-reduction of transition metallic complex is induced to cause electrochemiluminescence, thereby providing a method for detecting nucleic acid hybridization without a special labeling.

公开(公告)号：US06810713B2

公开(公告)日：2004-11-02

申请号：US10195320

申请日：2002-07-16

申请人： Jong Hoon Hahn ,  Kwanscop Lim ,  Kihoon Na ,  Suhyeon Kim ,  Je-Kyun Park

发明人： Jong Hoon Hahn ,  Kwanscop Lim ,  Kihoon Na ,  Suhyeon Kim ,  Je-Kyun Park

IPC分类号： G01N100

CPC分类号： G01N30/32 ,  F04B43/043 ,  F04B43/12 ,  G01N2030/326 ,  Y10T436/2575

摘要： Various kinds of fluid can be handled and delivered accurately in infinitesimal amounts through microchannels formed in an elastic polymeric substrate by periodically squeezing selective portions of the microchannel using a rotor to apply an external force on the elastic substrate. The delivery rate of the fluid is in the range from pL/sec to mL/sec determined by the rotation speed of the rotor which presses the microchannels and the dimensions of the microchannel. Regardless of physical properties of the fluid to be delivered, various kinds of fluids can be accurately delivered through the microchannels in the desired amount.

摘要翻译： 可以通过使用转子周期性地挤压微通道的选择性部分来在弹性基底上施加外力，可以以无限量的量以极小的量精确地处理和传送各种流体，通过形成在弹性聚合物基底中的微通道。 流体的输送速率在由按压微通道的转子的旋转速度和微通道的尺寸确定的pL / sec至mL / sec的范围内。 不管要输送的流体的物理性质如何，可以通过微通道以期望的量准确地输送各种流体。

公开(公告)号：US06391625B1

公开(公告)日：2002-05-21

申请号：US09604405

申请日：2000-06-27

申请人： Je Kyun Park ,  Tae Han Kim

发明人： Je Kyun Park ,  Tae Han Kim

IPC分类号： C12M134

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00536 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  C40B40/06 ,  C40B40/10 ,  C40B70/00 ,  G01N33/54373

摘要： A biochip including a DNA chip and a protein chip and a method for patterning and measuring biomaterial of the same are disclosed. The method for patterning a biomaterial of the biochip including a reflecting layer and an active layer on a substrate includes the steps of: rotating the biochip; successively irradiating pulse type laser beams to the rotating biochip to activate predetermined regions of the active layer; and fixing a biomaterial pattern on the activated predetermined regions. A method for measuring the patterned biomaterial includes the steps of: reacting a biomaterial labeled with at least one dye material with the biochip; rotating the biochip reacted with at least one biomaterial; successively irradiating laser beams to the rotating biochip; and detecting and processing light derived from the biochip as a result of reaction of the biomaterial to measure the biomaterial. Thus, the biochip having reliability and high packing density can be fabricated at low cost and the biomaterial of the biochip can be measured at high speed without using expensive equipments.

摘要翻译： 公开了一种包括DNA芯片和蛋白质芯片的生物芯片及其生物材料的图形化和测量方法。 在基板上包括反射层和有源层的生物芯片的生物材料图形化方法包括以下步骤：旋转生物芯片; 向旋转的生物芯片依次照射脉冲型激光束，激活有源层的预定区域; 以及在活化的预定区域上固定生物材料图案。 用于测量图案化生物材料的方法包括以下步骤：使用至少一种染料材料标记的生物材料与生物芯片反应; 旋转所述生物芯片与至少一种生物材料反应; 将激光束依次照射到旋转的生物芯片上; 并且由于生物材料的反应而测量和处理来自生物芯片的光，以测量生物材料。 因此，可以以低成本制造具有可靠性和高填充密度的生物芯片，并且可以高速度地测量生物芯片的生物材料而不使用昂贵的设备。

公开(公告)号：US5656142A

公开(公告)日：1997-08-12

申请号：US276097

申请日：1994-07-15

申请人： Je Kyun Park ,  Hee Jin Lee

发明人： Je Kyun Park ,  Hee Jin Lee

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N25/22 ,  G01N27/416 ,  G01N27/26

CPC分类号： C12Q1/002 ,  Y10S435/817

摘要： A biosensor for measuring gas-phase organic chemicals rapidly and accurately. The biosensor includes a thick film electrochemical device having an insulating substrate, electrodes formed on the substrate, connecting pads for the electrodes and an insulating layer, and a responsive membrane formed on the electrode portion of the electrochemical device. The responsive membrane is provided with a moisture-absorbing gel layer in which an enzyme for reacting with the gas-phase organic chemical is immobilized.

摘要翻译： 快速准确地测量气相有机化学物质的生物传感器。 生物传感器包括具有绝缘基板的厚膜电化学装置，形成在基板上的电极，用于电极的连接焊盘和绝缘层，以及形成在电化学装置的电极部分上的响应膜。 响应膜具有固定有与气相有机化学物质反应的酶的吸湿凝胶层。

公开(公告)号：US5571395A

公开(公告)日：1996-11-05

申请号：US334128

申请日：1994-11-04

申请人： Je Kyun Park ,  Hee Jin Lee

发明人： Je Kyun Park ,  Hee Jin Lee

IPC分类号： C12Q1/00 ,  C12Q1/32 ,  G01N27/327 ,  G01N27/416 ,  G01N33/497 ,  G01N27/26

CPC分类号： G01N33/4972 ,  C12Q1/005 ,  Y10S435/817

摘要： A biosensor for measuring alcohol concentration includes an insulating substrate and an amperometric device formed on the insulation substrate, having a plurality of conductive lines and connective pads and a plurality of electrodes. An enzyme immobilized layer is formed on one of the plurality of electrodes of the amperometric device and an enzyme paste is printed on the amperometric device. An outer membrane is formed on the substrate having the plurality of electrodes for forming an electrode system and an insulating membrane is formed on the substrate, except on the outer membrane.

摘要翻译： 用于测量醇浓度的生物传感器包括绝缘基板和形成在绝缘基板上的电流测量装置，具有多个导线和连接焊盘以及多个电极。 在电流分析装置的多个电极之一上形成酶固定化层，并且在电流分析装置上印刷酶糊剂。 在具有用于形成电极系统的多个电极的基板上形成外膜，除了外膜之外，在基板上形成绝缘膜。

公开(公告)号：US08465987B2

公开(公告)日：2013-06-18

申请号：US12746730

申请日：2008-11-19

申请人： Je-Kyun Park ,  Joo-Hun Kang ,  Sung-Young Choi ,  Won-Hye Lee

发明人： Je-Kyun Park ,  Joo-Hun Kang ,  Sung-Young Choi ,  Won-Hye Lee

IPC分类号： B03C1/02 ,  B03C1/32

CPC分类号： B03C1/32 ,  B01L3/502761 ,  B03C1/288 ,  B03C2201/18 ,  Y10T436/25375

摘要： The present invention relates to a method of separating fine particles by measuring the magnetic susceptibilities thereof using isomagnetophoresis. In a system for separating fine particles using isomagnetophoresis according to the present invention, fluids having different magnetic susceptibilities and fine particles to be measured are introduced into a microfluidic channel to form a magnetic susceptibility gradient, a strong magnetic field is applied to the channel to control the behavior of the introduced fine particles, thus moving the fine particles to respective positions at which the fluids having magnetic susceptibilities identical to those thereof is present. According to the present invention, fine particles having a fine difference in magnetic susceptibility can be separated from each other by measuring the magnetic susceptibilities thereof.

摘要翻译： 本发明涉及通过使用等离子体渗透法测定其磁敏度来分离细颗粒的方法。 在根据本发明的使用等离子体蒸气分离细颗粒的系统中，将具有不同磁化率的流体和要测量的细颗粒的流体引入微流体通道中以形成磁化率梯度，将强磁场施加到通道以控制 引入的细颗粒的行为，从而将细颗粒移动到具有与其相同的磁化率的流体的各个位置。 根据本发明，可以通过测量其磁化率来分离具有微小磁化率​​差的细颗粒。