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公开(公告)号：US07273745B2
公开(公告)日：2007-09-25
申请号：US11605148
申请日：2006-11-28
申请人： Lene Nonboe Andersen , Hanne Dela, legal representative , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (amino acids 236-242 of SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译： 本发明涉及属于多糖裂解酶家族1的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）（SEQ ID NO：2的氨基酸236-242）的果胶酸裂解酶在中性的工业过程中具有良好的性能 或碱性条件如洗涤和纺织加工。 果胶裂解酶可以衍生自芽孢杆菌属物种。
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公开(公告)号：US07226773B2
公开(公告)日：2007-06-05
申请号：US10965499
申请日：2004-10-14
申请人： Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
发明人： Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
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公开(公告)号：US06428993B1
公开(公告)日：2002-08-06
申请号：US08881742
申请日：1997-06-24
申请人： Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
发明人： Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
IPC分类号： C12N910
CPC分类号： C12Y203/02013 , A21D8/042 , C12N9/1044 , C14C11/00 , Y10S435/911
摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
摘要翻译： 本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。 通过生长这些细胞形成的细菌，特别是菌株腐霉菌，不锈钢腐霉，腐霉腐霉（P. periplocum），腐皮腐，腐霉，腐霉，腐皮疫霉，致病疫霉（Phytophthora palmivora），可以实现前所未有的高水平表达 ，疫霉疫霉（Phytophthora porri），致病疫霉（Phytophthora infestans），致病疫霉（Phytoph-thora megasperma），肉桂疫霉（Phytophthora cinnamomi）和疫霉疫霉（Phytophthora cryptogea）。 克隆和表达重组转谷氨酰胺酶，酶和酶制剂可用于交联蛋白质，例如， 在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。 在面包和其他烘烤含麸质的食品中。
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公开(公告)号：US06368843B1
公开(公告)日：2002-04-09
申请号：US09694531
申请日：2000-10-23
申请人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号： C12N926
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译： 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可以衍生自芽孢杆菌属物种。
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公开(公告)号：US06329185B1
公开(公告)日：2001-12-11
申请号：US09129033
申请日：1998-08-04
IPC分类号： C12P2106
CPC分类号： C12Y302/01089 , A23K20/189 , A23L2/62 , A23L2/84 , A23L29/06 , C12G1/0203 , C12N9/2468 , C12N9/2471 , C12Y302/01023 , D21C5/005
摘要： The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme composition comprising the enzyme, and the use of the enzyme and enzyme composition for a number of industrial applications.
摘要翻译： 本发明涉及具有半乳聚糖酶活性的酶，编码该酶的DNA构建体，该酶的制备方法，包含该酶的酶组合物以及该酶和酶组合物在许多工业应用中的用途。
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公开(公告)号：US06190905B1
公开(公告)日：2001-02-20
申请号：US09408257
申请日：1999-09-29
申请人： Henrik Dalbøge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
发明人： Henrik Dalbøge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
IPC分类号： D06M1600
CPC分类号： C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要： An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO: 1 or 2. Methods are described for using the protease compositions in reducing vescosity, cleaning contact lenses, baking, and preparing animal feed.
摘要翻译： 在4-7的pH下显示出蛋白酶活性的分离和纯化的酶，其在5％过氧化氢中显示蛋白酶，并且由与SEQ ID NO：1或2所示的DNA序列杂交的DNA序列编码。描述方法 用于使用蛋白酶组合物减少紫外线，清洁隐形眼镜，烘烤和制备动物饲料。
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公开(公告)号：US06187580B1
公开(公告)日：2001-02-13
申请号：US09198955
申请日：1998-11-24
申请人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号： C12N988
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.
摘要翻译： 属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。 果胶裂解酶可从芽孢杆菌属物种衍生。
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公开(公告)号：US06159718A
公开(公告)日：2000-12-12
申请号：US087194
申请日：1998-05-29
申请人： Henrik Dalboege , Lene Nonboe Andersen , Lene Venke Kofoed , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Torben Halkier
发明人： Henrik Dalboege , Lene Nonboe Andersen , Lene Venke Kofoed , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Torben Halkier
CPC分类号： C12G1/0203 , C12N9/2402 , C12Y302/01015
摘要： An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.
摘要翻译： 显示多聚半乳糖醛酸酶活性的酶，该酶与针对源自Aspergillus aculeatus的纯化多聚半乳糖醛酸酶CBS 101.43产生的抗体具有免疫反应性。 该酶可以通过重组DNA技术产生并且可以用于植物细胞壁的降解，例如在葡萄酒和果汁生产中。
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公开(公告)号：US6143546A
公开(公告)日：2000-11-07
申请号：US342394
申请日：1999-06-29
申请人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
发明人： Markus Sakari Kauppinen , Joan Qi Si , Tina Spendler , Claus Dambmann , Torben Halkier , Peter Rahbek stergaard , Shamkant Anant Patkar , Kim Hansen
IPC分类号： G02C13/00 , A21D8/04 , A23J3/34 , C07H21/04 , C11D3/386 , C11D7/42 , C12N1/16 , C12N1/19 , C12N9/48 , C12N15/00 , C12N15/09 , C12R1/19 , C12R1/69
摘要： The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.
摘要翻译： 本发明涉及一种表现出氨基肽酶活性的酶，一种生产该酶的方法，一种含有显示氨基肽酶活性的酶的酶制剂，以及所述酶用于各种工业目的。
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公开(公告)号：US5885819A
公开(公告)日：1999-03-23
申请号：US902655
申请日：1997-07-30
申请人： Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
发明人： Lene Venke Kofod , Markus Sakari Kauppinen , Stephan Christgau , Hans Peter Heldt-Hansen , Henrik Dalb.o slashed.ge , Lene Nonboe Andersen , Joan Qi Si , Tina Sejersg.ang.rd Jacobsen , Niels Munk , Anette Mullertz
IPC分类号： A21D8/04 , A23K1/165 , A23K1/18 , A23L1/03 , A23L1/105 , A23L2/04 , C12C5/00 , C12C7/28 , C12G1/00 , C12G1/022 , C12N9/24 , C12N15/56 , D21C5/00 , C07H21/04 , C12N1/22 , C12N15/00
CPC分类号： C12Y302/01032 , A21D8/042 , A23K20/189 , A23K50/75 , A23L2/04 , A23L29/06 , A23L7/107 , C12C5/004 , C12C5/006 , C12C7/28 , C12G1/00 , C12G1/0203 , C12N9/2482 , C12Y302/01008 , D21C5/005
摘要： An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components e.g. in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.
摘要翻译： 显示出木聚糖酶活性的酶，该酶与针对源自Aspergillus aculeatus，CBS 101.43的纯化木聚糖酶产生的抗体具有免疫反应性。 该酶可用于降解植物细胞壁组分，例如 在制备饲料时，在烘烤中，在造纸和纸浆工业中，并将小麦分离成淀粉和面筋。
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