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    Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
    4.
    发明申请
    Methods for production of arrays with modified oligonucleotide and polynucleotide compositions 审中-公开
    用修饰的寡核苷酸和多核苷酸组合物制备阵列的方法

    公开(公告)号：US20050191655A1

    公开(公告)日：2005-09-01

    申请号：US10985655

    申请日：2004-11-09

    申请人： Roderic Dale

    发明人： Roderic Dale

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6837

    摘要： The present invention provides methods for producing arrays having associated modified nucleic acid structures, e.g. acid stable and/or end-blocked nucleic acids such as 2′-O—R oligonucleotides. In one embodiment, arrays produced using the methods of the invention exhibit an increased binding affinity with complementary nucleic acids, and in particular with complementary RNA. In another embodiment, the associated nucleic acids of the array produced using the methods of the invention exhibit substantial acid resistance, allowing the arrays to be treated with low pH solutions. In another embodiment, the modified associated nucleic acids of the array produced using the methods of the invention exhibit substantial resistance to nuclease degradation.

    摘要翻译： 本发明提供了制备具有相关联的修饰的核酸结构的阵列的方法，例如 酸稳定的和/或端封闭的核酸如2'-O-R寡核苷酸。 在一个实施方案中，使用本发明的方法产生的阵列表现出与互补核酸，特别是与互补RNA的增加的结合亲和力。 在另一个实施方案中，使用本发明的方法制备的阵列的相关核酸表现出显着的耐酸性，允许阵列用低pH值溶液处理。 在另一个实施方案中，使用本发明的方法制备的阵列的修饰的相关核酸表现出对核酸酶降解的显着抗性。

    Method for detecting nucleic acid sequences
    5.
    发明申请
    Method for detecting nucleic acid sequences 审中-公开
    检测核酸序列的方法

    公开(公告)号：US20050118618A1

    公开(公告)日：2005-06-02

    申请号：US10937112

    申请日：2004-09-08

    申请人： Roderic Dale

    发明人： Roderic Dale

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q2525/113

    摘要： A method for detecting nucleic acid sequences in two or more collections of nucleic acid molecules, the method comprising: (a) providing an array of modified polynucleotides bound to a solid surface, each said modified polynucleotide comprising a determinable nucleic acid; (b) contacting the array of modified polynucleotides with: (i) a first collection of labeled nucleic acid comprising a sequence substantially complementary to a nucleic acid of said array, and (ii) at least a second collection of labeled nucleic acid comprising a sequence substantially complementary to a modified polynucleotide of said array; wherein the first and second labels are distinguishable from each other; and (c) detecting hybridization of the first and second labeled complementary nucleic acids to nucleic acids of said arrays; wherein the modified oligonucleotides are characterized by a characteristic selected from the group consisting of (a) a binding affinity of at least about 1.25 times that of a corresponding, non-modified oligonucleotide, (b) a pH stability of at least one hour at 37 C at a pH in a range of about 0.5 to 10; and (c) a nuclease resistance of at least twice that of a naturally occurring oligonucleotide having the same sequence and number of bases.

    摘要翻译： 一种用于检测两个或更多个核酸分子集合中的核酸序列的方法，所述方法包括：（a）提供与固体表面结合的修饰多核苷酸阵列，每个所述修饰的多核苷酸包含可测定的核酸; （b）将经修饰的多核苷酸阵列与以下物质接触：（i）包含与所述阵列的核酸基本上互补的序列的标记核酸的第一次收集，和（ii）至少第二次标记的核酸集合，其包含序列 基本上与所述阵列的修饰的多核苷酸互补; 其中所述第一和第二标签彼此可区分; 和（c）检测第一和第二标记的互补核酸与所述阵列的核酸的杂交; 其中修饰的寡核苷酸的特征在于选自（a）至少约1.25倍相应未修饰寡核苷酸的结合亲和力的特征，（b）在37℃下至少1小时的pH稳定性 pH在约0.5至10的范围内; 和（c）具有相同序列和碱基数的天然存在的寡核苷酸的至少两倍的核酸酶抗性。

    METHOD FOR DETECTING NUCLEIC ACID SEQUENCES
    6.
    发明申请
    METHOD FOR DETECTING NUCLEIC ACID SEQUENCES 审中-公开
    检测核酸序列的方法

    公开(公告)号：US20060223083A1

    公开(公告)日：2006-10-05

    申请号：US11277750

    申请日：2006-03-28

    申请人： Roderic Dale

    发明人： Roderic Dale

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q2525/113

    摘要： A method for detecting nucleic acid sequences in two or more collections of nucleic acid molecules, the method comprising: (a) providing an array of modified polynucleotides bound to a solid surface, each said modified polynucleotide comprising a determinable nucleic acid; (b) contacting the array of modified polynucleotides with: (i) a first collection of labeled nucleic acid comprising a sequence substantially complementary to a nucleic acid of said array, and (ii) at least a second collection of labeled nucleic acid comprising a sequence substantially complementary to a modified polynucleotide of said array; wherein the first and second labels are distinguishable from each other; and (c) detecting hybridization of the first and second labeled complementary nucleic acids to nucleic acids of said arrays; wherein the modified oligonucleotides are characterized by a characteristic selected from the group consisting of (a) a binding affinity of at least about 1.25 times that of a corresponding, non-modified oligonucleotide, (b) a pH stability of at least one hour at 37 C at a pH in a range of about 0.5 to 10; and (c) a nuclease resistance of at least twice that of a naturally occurring oligonucleotide having the same sequence and number of bases.

    摘要翻译： 一种用于检测两个或更多个核酸分子集合中的核酸序列的方法，所述方法包括：（a）提供与固体表面结合的修饰多核苷酸阵列，每个所述修饰的多核苷酸包含可测定的核酸; （b）将经修饰的多核苷酸阵列与以下物质接触：（i）包含与所述阵列的核酸基本上互补的序列的标记核酸的第一次收集，和（ii）至少第二次标记的核酸集合，其包含序列 基本上与所述阵列的修饰的多核苷酸互补; 其中所述第一和第二标签彼此可区分; 和（c）检测第一和第二标记的互补核酸与所述阵列的核酸的杂交; 其中修饰的寡核苷酸的特征在于选自（a）至少约1.25倍相应未修饰寡核苷酸的结合亲和力的特征，（b）在37℃下至少1小时的pH稳定性 pH在约0.5至10的范围内; 和（c）具有相同序列和碱基数的天然存在的寡核苷酸的至少两倍的核酸酶抗性。

    Antimicrobial and Antiviral Compounds and Methods for their Use
    7.
    发明申请
    Antimicrobial and Antiviral Compounds and Methods for their Use 有权
    抗微生物和抗病毒化合物及其使用方法

    公开(公告)号：US20070053971A1

    公开(公告)日：2007-03-08

    申请号：US11552951

    申请日：2006-10-25

    申请人： Roderic Dale

    发明人： Roderic Dale

    IPC分类号： A61K31/7076 ,  A61K31/7072 ,  A61K9/127

    CPC分类号： A61K9/0014 ,  A61K9/1271 ,  A61K31/7072 ,  A61K31/7076 ,  C07H11/04 ,  C07H19/10 ,  Y02A50/473 ,  Y02A50/475 ,  Y02A50/481

    摘要： The present invention provides protonated compounds having antiviral and antimicrobial activity. The invention also provides antimicrobial compositions comprising protonated compounds of the invention. The protonated compounds of the invention provide efficacious antimicrobial activity against resistant strains of bacteria and opportunistic fungi. The invention also provides antiviral compositions comprising compounds of the invention. Viruses that may be treated by compositions of the invention include, but are not limited to, HIV, HSV, CMV, HBV, HCV and influenza virus.

    摘要翻译： 本发明提供具有抗病毒和抗微生物活性的质子化化合物。 本发明还提供包含本发明的质子化化合物的抗微生物组合物。 本发明的质子化化合物为细菌和机会真菌的抗性菌株提供有效的抗微生物活性。 本发明还提供包含本发明化合物的抗病毒组合物。 可以通过本发明的组合物治疗的病毒包括但不限于HIV，HSV，CMV，HBV，HCV和流感病毒。

    Method for nucleic acid preparation
    9.
    发明申请
    Method for nucleic acid preparation 审中-公开
    核酸制备方法

    公开(公告)号：US20050136458A1

    公开(公告)日：2005-06-23

    申请号：US10984030

    申请日：2004-09-14

    申请人： Roderic Dale ,  Steven Gatton

    发明人： Roderic Dale ,  Steven Gatton

    IPC分类号： C07H21/04 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/101 ,  C07H21/04 ,  C12Q1/6806

    摘要： Concentration of oligonucleotides in salt solutions is accomplished by loading a solution of the oligonucleotide dissolved in aqueous sodium chloride or other salt solution onto a reverse-phase poly(styrene-divinylbene) liquid chromatography (LC) column. The column bearing oligonucleotide is then washed with water to low conductivity and eluted with an organic eluent such as ethanol, thus effecting a combination desalting concentration procedure in one step, thus this procedure has utility in the desalting and concentration of oligonucleotides that have been purified and/or treated by anion exchange chromatography. In situ cationic exchange of the associated cation of the oligonucleotide can also be incorporated into the procedure of the new invention.

    摘要翻译： 盐溶液中寡核苷酸的浓度通过将溶解在氯化钠水溶液或其它盐溶液中的寡核苷酸溶液加载到反相聚（苯乙烯 - 二乙烯基苯）液相色谱（LC）柱上来完成。 然后将带有柱的寡核苷酸用水洗涤至低电导率，并用有机洗脱液如乙醇洗脱，从而在一步中实现脱盐浓缩程序的组合，因此该方法可用于脱盐和浓缩已经纯化的寡核苷酸， /或通过阴离子交换色谱法处理。 寡核苷酸的相关阳离子的原位阳离子交换也可以并入新发明的方法中。