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    Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins
    5.
    发明授权
    Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins 失效
    使用硫醇氧化还原蛋白来减少蛋白质分子内二硫键，用于提高谷物，面团和烘焙食品的质量，以及灭活蛇，蜜蜂和蝎毒素

    公开(公告)号：US06610334B1

    公开(公告)日：2003-08-26

    申请号：US09447615

    申请日：1999-11-23

    申请人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee

    发明人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee

    IPC分类号： A21D1000

    CPC分类号： C12P21/02 ,  A21D2/26 ,  A21D2/265 ,  A21D2/266 ,  A21D8/04 ,  A23C9/1213 ,  A23J1/12 ,  A23J1/14 ,  A23J3/18 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A23L11/34 ,  A23L13/48 ,  A61K38/00 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y02A50/473 ,  A61K38/44 ,  A61K2300/00

    摘要： Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or E. coli. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.

    摘要翻译： 提供了减少含有动物和植物蛋白质的方法，以及改善面团和焙烤食品的特性，包括将生面团成分与硫醇氧化还原蛋白混合以形成面团并烘焙面团以形成烤制品的步骤。 本发明的方法优选使用减少的硫氧还蛋白与小麦粉，其赋予更强的面团和更高的面包体积。 还提供了用硫醇氧化还原（SH）试剂降低蛇，蜂和蝎毒素蛋白，从而使个体灭活蛋白或使蛋白质解毒的方法。 包括大豆的Kunitz和Bowman-Birk胰蛋白酶抑制剂的蛋白酶抑制剂也被来自大肠杆菌或小麦胚芽的NADP /硫氧还蛋白体系（NADPH，硫氧还蛋白和NADP-硫氧还蛋白还原酶）还原。 当被硫氧还蛋白减少时，Kunitz和Bowman-Birk大豆胰蛋白酶抑制剂失去了抑制胰蛋白酶的能力。 此外，还原形式的抑制剂显示由发芽小麦种子中的枯草杆菌蛋白酶或蛋白酶制剂对热和蛋白水解的敏感性增加。 蓖麻种子胚乳的2S白蛋白由小麦胚芽或大肠杆菌的硫氧还蛋白降低。 硫氧还蛋白被NADPH和NADP硫氧还蛋白还原酶或二硫苏糖醇还原。 分析表明，硫氧还蛋白活性降低了2S大亚基的分子内二硫化物，但在减少连接大亚基与小亚基的分子间二硫化物方面无效。 分离出含有蛋白质的新型胱氨酸，其抑制支链淀粉酶。 蛋白质被硫氧还蛋白还原，当其还原时，其抑制活性被破坏或大大降低。

    Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins
    7.
    发明授权
    Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins 失效
    使用硫醇氧化还原蛋白来减少蛋白质分子内二硫键，用于提高谷物，面团和烘焙食品的质量，以及灭活蛇，蜜蜂和蝎毒素

    公开(公告)号：US06583271B1

    公开(公告)日：2003-06-24

    申请号：US09448109

    申请日：1999-11-23

    申请人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee

    发明人： Bob B. Buchanan ,  Karoly Kobrehel ,  Boihon C. Yee

    IPC分类号： A23J112

    CPC分类号： C12P21/02 ,  A21D2/26 ,  A21D2/265 ,  A21D2/266 ,  A21D8/04 ,  A23C9/1213 ,  A23J1/12 ,  A23J1/14 ,  A23J3/18 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A23L11/34 ,  A23L13/48 ,  A61K38/00 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y02A50/473 ,  A61K38/44 ,  A61K2300/00

    摘要： Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or E. coli. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.

    摘要翻译： 提供了减少含有动物和植物蛋白质的方法，以及改善面团和焙烤食品的特性，包括将生面团成分与硫醇氧化还原蛋白混合以形成面团并烘焙面团以形成烤制品的步骤。 本发明的方法优选使用减少的硫氧还蛋白与小麦粉，其赋予更强的面团和更高的面包体积。 还提供了用硫醇氧化还原（SH）试剂降低蛇，蜂和蝎毒素蛋白，从而使个体灭活蛋白或使蛋白质解毒的方法。 包括大豆Kunitz和Bowman-Birk胰蛋白酶抑制剂在内的蛋白酶抑制剂也被来自大肠杆菌或小麦胚芽的NADP /硫氧还蛋白系统（NADPH，硫氧还蛋白和NADP-硫氧还蛋白还原酶）还原。 当被硫氧还蛋白减少时，Kunitz和Bowman-Birk大豆胰蛋白酶抑制剂失去了抑制胰蛋白酶的能力。 此外，还原形式的抑制剂显示由发芽小麦种子中的枯草杆菌蛋白酶或蛋白酶制剂对热和蛋白水解的敏感性增加。 蓖麻种子胚乳的2S白蛋白由小麦胚芽或大肠杆菌的硫氧还蛋白降低。 硫氧还蛋白被NADPH和NADP硫氧还蛋白还原酶或二硫苏糖醇还原。 分析表明，硫氧还蛋白活性降低了2S大亚基的分子内二硫化物，但在减少连接大亚基与小亚基的分子间二硫化物方面无效。 分离出含有蛋白质的新型胱氨酸，其抑制支链淀粉酶。 蛋白质被硫氧还蛋白还原，当其还原时，其抑制活性被破坏或大大降低。