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    Mass biosensor method with quantified sensor renewal
    2.
    发明授权
    Mass biosensor method with quantified sensor renewal 失效
    大量生物传感器方法与定量传感器更新

    公开(公告)号：US5229301A

    公开(公告)日：1993-07-20

    申请号：US711786

    申请日：1991-06-07

    Applicant: Darlene J. Spira-Solomon ,  May Tom-Moy ,  Carl A. Myerholtz

    Inventor： Darlene J. Spira-Solomon ,  May Tom-Moy ,  Carl A. Myerholtz

    IPC: G01N21/64 ,  G01N21/76 ,  G01N33/543

    CPC classification number: G01N33/54373 ,  Y10S436/80 ,  Y10S436/805

    Abstract: A mass biosensor method provides enhanced quantification of analyte concentrations in a sample. In a direct approach, an analyte is derivatized to form an analyte chelate and then specifically bound to a sensor. In an indirect approach, a complement of the analyte is derivatized to form a complement chelate which is then bound to a sensor. In a direct/indirect hybrid approach, an analog of the analyte is derivatized to form an analog chelate that is bound to a sensor in competition with the sample analyte. In all three approaches, mass measurements taken as the ligand chelate attaches to the sensor permit the concentration of the analyte in the sample to be calculated. Once measurement is completed, a dissociation treatment is applied to dissociate the derivatized species from the sensor so that the sensor can be reused. The effects of the dissociation treatment can be monitored using phosphorescence detection. The results obtained during monitoring can be compared with a predetermined threshold to ensure complete dissociation while avoiding alteration of the sensor surface. This procedure permits precision renewal of a sensor to maximize the number of times a sensor can be used. Moreover, this method allows quantification to be performed using the same sensor and coating in place during calibration, minimizing systematic errors and enhancing quantification accuracy.

    Substrate preparation for chemical-species-specific binding
    4.
    发明授权
    Substrate preparation for chemical-species-specific binding 失效

    公开(公告)号：US06586232B2

    公开(公告)日：2003-07-01

    申请号：US09814360

    申请日：2001-03-21

    Applicant: May Tom-Moy ,  Carl Alan Myerholtz

    Inventor： May Tom-Moy ,  Carl Alan Myerholtz

    IPC: C12Q168

    CPC classification number: G01N33/54373 ,  G01N29/022 ,  G01N33/54353 ,  G01N33/552 ,  G01N2291/0256 ,  G01N2291/0422 ,  G01N2291/0423 ,  G01N2291/0427 ,  Y10S436/827

    Abstract: A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.

    Surface preparation for chemical-specific binding
    5.
    发明授权
    Surface preparation for chemical-specific binding 失效
    化学特异性结合的表面处理

    公开(公告)号：US06235488B1

    公开(公告)日：2001-05-22

    申请号：US07876804

    申请日：1992-04-29

    Applicant: May Tom-Moy ,  Carl Alan Myerholtz

    Inventor： May Tom-Moy ,  Carl Alan Myerholtz

    IPC: G01N3353

    CPC classification number: G01N33/54373 ,  G01N29/022 ,  G01N33/54353 ,  G01N33/552 ,  G01N2291/0256 ,  G01N2291/0422 ,  G01N2291/0423 ,  G01N2291/0427 ,  Y10S436/827

    Abstract: A mass biosensor uses an intermediate avidin layer to facilitate binding of a biotinylated antibody to a measurement surface of the biosensor. The avidin layer can be added by the manufacturer of the biosensor, while the biotinylated layer can be added by the user. This two-phase method of chemically modifying the measurement surface significantly reduces the user time required to customize the measurement surface to render it capable of binding selected compounds. An organosilane coupling agent attached to the surface provides sites to which avidin is bound. Avidin acts as a universal receptor of biotinylated compounds with specific binding affinities. Biotinylated antibodies or other biotinylated compounds are added and bind to the immobilized avidin. Surface adsorption is reduced by washing the modified surface with biotin to block potential sites of weak bond formation, electrostatic and hydrophobic interactions.

    Abstract translation: 质量生物传感器使用中间抗生物素蛋白层来促进生物素化抗体与生物传感器的测量表面的结合。 生物传感器的制造商可以添加亲和素层，而生物素化层可以由用户添加。 这种化学修饰测量表面的两相方法显着地减少了定制测量表面以使其能够结合所选化合物所需的用户时间。 附着在表面上的有机硅烷偶联剂提供结合抗生物素蛋白的位点。 亲和素作为具有特异性结合亲和力的生物素化化合物的通用受体。 加入生物素化抗体或其他生物素化化合物并结合固定的抗生物素蛋白。 通过用生物素洗涤修饰的表面来减少表面吸附以阻断弱键形成，静电和疏水相互作用的潜在位点。

    Biomolecular sensors and detection methods utilizing photoinduced charge separation
    9.
    发明申请
    Biomolecular sensors and detection methods utilizing photoinduced charge separation 审中-公开
    生物分子传感器和利用光诱导电荷分离的检测方法

    公开(公告)号：US20050255515A1

    公开(公告)日：2005-11-17

    申请号：US11153000

    申请日：2005-06-14

    Applicant: Daniel Roitman ,  May Tom-Moy ,  Seiji Inaoka ,  Robert Ach

    Inventor： Daniel Roitman ,  May Tom-Moy ,  Seiji Inaoka ,  Robert Ach

    IPC: G01N33/543 ,  C12Q1/68

    CPC classification number: G01N33/54373

    Abstract: The invention provides methods and sensors for detecting target biological molecules. Biosensors feature photoactivatable charge separation moieties capable of generating electron-hole pairs upon photoinduction. Photoinduced charge carriers participate in redox reactions that are detectable, for example, by optical, chemical, or electronic means.

    Abstract translation: 本发明提供了用于检测靶生物分子的方法和传感器。 生物传感器具有能够在光诱导时产生电子 - 空穴对的可光活化的电荷分离部分。 光诱导电荷载体参与例如通过光学，化学或电子手段可检测的氧化还原反应。

    Detection of molecular binding events
    10.
    发明申请
    Detection of molecular binding events 审中-公开
    检测分子结合事件

    公开(公告)号：US20050069905A1

    公开(公告)日：2005-03-31

    申请号：US10676957

    申请日：2003-09-30

    Applicant: Carl Myerholtz ,  May Tom-Moy ,  August Hidalgo ,  William McAllister

    Inventor： Carl Myerholtz ,  May Tom-Moy ,  August Hidalgo ,  William McAllister

    IPC: C12Q1/68 ,  G01N33/543 ,  C12M1/34

    CPC classification number: G01N33/5438 ,  C12Q1/6825

    Abstract: A device for electronic detection of a target includes a probe, e.g. an oligonucleotide, attached to a pad of resistive material, wherein the pad is adjacent a first electrode and also is adjacent a second electrode. In use, the probe is contacted with a sample containing the target, e.g. a target nucleic acid, under conditions and for a time sufficient to allow target to bind the probe. An enhancement reaction is then applied to result in a change in an observable property of the device. The observable property is then monitored using measurement apparatus operably associated with the device. Typically, multiple devices will be present on an array of devices, allowing multiplex analysis of multiple different targets using a single array of devices.

    Abstract translation: 用于电子检测靶的装置包括探针，例如。 附着到电阻材料焊盘的寡核苷酸，其中所述焊盘与第一电极相邻并且还邻近第二电极。 在使用中，探针与含有靶标的样品接触。 在足以允许靶结合探针的条件下和足够时间的靶核酸。 然后应用增强反应以导致装置的可观察性质的变化。 然后使用与装置可操作地相关联的测量装置监测可观察的性质。 通常，多个设备将存在于设备阵列上，允许使用单个阵列阵列对多个不同目标进行多路复用分析。

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