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公开(公告)号：US07111635B2
公开(公告)日：2006-09-26
申请号：US10269476
申请日：2002-10-11
CPC分类号： B81C1/00119 , B01L3/502707 , B01L3/502753 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B81B2201/058 , B81B2203/0361 , Y10T137/0396 , Y10T137/2076 , Y10T137/2191 , Y10T137/2196 , Y10T137/2224
摘要： A method is provided for fabricating a constriction region in a channel of a microfluidic device. The method includes the steps of introducing a pre-polymer mixture including a monomer, cross-linking agent and photoinitiator into the channel. The pre-polymer mixture is polymerized at a localized area of the channel so as to shrink and solidify the liquid mixture. The solidified and shrunken liquid mixture provides the constriction region in the channel.
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公开(公告)号：US07713736B2
公开(公告)日：2010-05-11
申请号：US10997259
申请日：2004-11-24
IPC分类号： C12N5/00
CPC分类号： B01L3/5027 , B01L2200/0668 , B01L2300/0822 , B01L2300/0877
摘要： A platform and method for mimicking the environment within a cell is provided. The platform includes a microfluidic device defining a chamber. At least one hydrogel post is positioned within the chamber of the microfluidic device. Each hydrogel post defines a corresponding pore for receiving a first molecule therein. Second molecules are introduced into the pores of the hydrogel posts and the interactions between the first and second molecules are observed.
摘要翻译： 提供了一种用于模拟单元内的环境的平台和方法。 平台包括限定腔室的微流体装置。 至少一个水凝胶柱位于微流体装置的腔室内。 每个水凝胶柱限定了用于在其中接收第一分子的相应的孔。 将第二分子引入水凝胶柱的孔中，观察第一和第二分子之间的相互作用。
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公开(公告)号：US07253003B2
公开(公告)日：2007-08-07
申请号：US10045937
申请日：2001-10-19
申请人： David J. Beebe , Jaisree Moorthy
发明人： David J. Beebe , Jaisree Moorthy
IPC分类号： G01N33/48
CPC分类号： B01L3/5027 , B01L2200/143 , B01L2300/0627 , G01N21/80 , Y10T436/2575
摘要： A method and apparatus is provided for monitoring the environment within a microfluidic device. The microfluidic device includes a body defining a channel for accommodating flow of fluid therethrough. A monitor structure is disposed in the channel of the body in the flow of fluid. The monitor structure changes color and/or dimension in response to various parameters of the fluid having predetermined values.
摘要翻译： 提供了一种用于监测微流体装置内的环境的方法和装置。 微流体装置包括限定用于容纳流体流过其中的通道的主体。 监测器结构设置在流体流动中的身体的通道中。 响应于具有预定值的流体的各种参数，监视器结构改变颜色和/或尺寸。
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公开(公告)号：US06488872B1
公开(公告)日：2002-12-03
申请号：US09625204
申请日：2000-07-21
申请人： David J. Beebe , Jeffrey S. Moore
发明人： David J. Beebe , Jeffrey S. Moore
IPC分类号： E04B116
CPC分类号： G03F7/00 , B01J19/0093 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00891 , B01J2219/00925 , B01J2219/00936 , B01J2219/00952 , B01J2219/00966 , B01L3/502707 , B01L3/502738 , B01L3/502746 , B01L3/502776 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0883 , B01L2300/16 , B01L2300/161 , B01L2400/0661 , G03F7/0035
摘要： Microfabricated devices and methods of manufacturing the devices are disclosed. The devices are manufactured from a substrate having microscale fluid channels, and polymerizing a polymerizable mixture in the channels to form stimuli-responsive operating components of the device. The operating components can be functional or structural components. The method of manufacture obviates the traditional assembly of microscale components to form a device because the microscale components are formed in situ on or within the device.
摘要翻译： 公开了微型装置和制造装置的方法。 这些装置由具有微量流体通道的基底制造，并且在通道中聚合可聚合混合物以形成装置的刺激响应性操作部件。 操作部件可以是功能或结构部件。 制造方法消除了微量元件的传统组装以形成器件，因为微量元件在器件上或器件内原位形成。
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公开(公告)号：US06821485B2
公开(公告)日：2004-11-23
申请号：US10071846
申请日：2002-02-08
申请人： David J. Beebe , Jeffrey S. Moore , Bin Zhao
发明人： David J. Beebe , Jeffrey S. Moore , Bin Zhao
IPC分类号： B01L302
CPC分类号： B82Y30/00 , B01L3/5027 , B01L3/502707 , B01L2300/089 , B01L2400/0672 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A flow of liquids is carried out on a microscale utilizing surface effects to guide the liquid on flow paths to maintain laminar flow. No sidewall confining structure is required, minimizing resistance to flow and allowing laminar flow to be maintained at high flow rates. The guiding structure has flow guiding stripes formed on one or both of facing base and cover surfaces which are wettable by a selected liquid to direct the liquid from a source location to a destination location. The regions adjacent to the guiding stripes on the base and cover surfaces are non-wettable. The smooth interface between the gas and liquid along the flowing stream allows gas-liquid reactions to take place as a function of diffusion across the interface without mixing of the gas and liquid. Liquid-liquid flows may also be guided with such structures.
摘要翻译： 使用表面效应在微尺度上进行液体流动以将液体引导到流动路径上以保持层流。 不需要侧壁约束结构，使流动阻力最小化，并允许层流维持在高流速。 引导结构具有形成在一个或两个相对的基底和盖表面上的流动引导条纹，其可被选定的液体润湿以将液体从源位置引导到目的地位置。 在基部和盖表面上与引导条相邻的区域是不可润湿的。 沿着流动流动的气体和液体之间的平滑界面允许气 - 液反应作为扩散跨越界面而发生，而不会混合气体和液体。 液 - 液流也可以用这种结构引导。
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公开(公告)号：US06523559B2
公开(公告)日：2003-02-25
申请号：US09917523
申请日：2001-07-27
IPC分类号： G05D1103
CPC分类号： G05D11/08 , G05D21/00 , Y10T137/0329 , Y10T137/2509
摘要： In accordance with the present invention, a microfluidic device and method of using the same is provided for self-regulating the flow of fluid therethrough. The microfluidic device includes a body defining first and second flow channels. The first flow channel has an input for receiving the fluid and an output. The second flow channel has an input for receiving a compensating fluid to modify the value of the property of the fluid and an output communicating with the first flow channel. A polymeric material is disposed in the first flow channel downstream of the output of the second flow channel. The polymeric material has a volume responsive to the value of the property of the fluid. A valve is disposed in the second flow channel and is movable in response to the volume of the material. The valve is movable between the first open position allowing the compensating fluid to flow past the valve into the first flow channel and a second closed position limiting the flow of compensating fluid therepast.
摘要翻译： 根据本发明，提供了一种微流体装置及其使用方法，用于自我调节流体的流动。 微流体装置包括限定第一和第二流动通道的主体。 第一流道具有用于接收流体和输出的输入。 第二流动通道具有用于接收补偿流体以修改流体性质的值的输入和与第一流动通道连通的输出。 聚合材料设置在第二流动通道的第二流动通道的输出的下游。 聚合物材料具有响应于流体性质的值的体积。 阀设置在第二流动通道中并且可以响应于材料的体积而移动。 所述阀可在所述第一打开位置和所述第一关闭位置之间移动，所述第一打开位置允许所述补偿流体流过所述阀进入所述第一流动通道，以及限制所述补偿流体的流动的第二关闭
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公开(公告)号：US20230242856A1
公开(公告)日：2023-08-03
申请号：US18131620
申请日：2023-04-06
申请人： David J. Beebe , Layla J. Barkal , Jason Yu
发明人： David J. Beebe , Layla J. Barkal , Jason Yu
摘要： A platform and method for testing antibiotic sensitivity of a polymicrobial infection is provided. The platform includes a body defining a plurality of sets of chambers and a plurality of wells. Each set of chambers has a plurality of chambers adapted for culturing microbes of the polymicrobial infection therein. Each well is associated with a corresponding set of chambers and has an input in fluidic communication with the outlets of the plurality of chambers in the corresponding set of chambers. Selected antibiotic therapies may be received in the wells which fluidically connect the plurality of chambers in a corresponding set of chambers such that microbes cultured in the plurality of chambers in the corresponding set of chambers are in soluble factor contact.
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公开(公告)号：US11430279B2
公开(公告)日：2022-08-30
申请号：US13467599
申请日：2012-05-09
摘要： A microfluidic platform and method are provided. The microfluidic platform includes a base having an outer surface and a plurality of wells formed in the outer surface thereof for receiving fluid therein. The plurality of wells are in fluid communication with each other. A lid includes a plurality of channels having corresponding inputs and outputs. The lid is moveable between a first position wherein the lid is disengaged from the base and a second position wherein the inputs of each channel communicate with corresponding wells in the base. The fluid in each well is drawn into corresponding channels through the inputs thereof by capillary action.
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公开(公告)号：US08728410B2
公开(公告)日：2014-05-20
申请号：US13033351
申请日：2011-02-23
IPC分类号： B03C1/02
CPC分类号： B01D21/0009 , B01D2221/10 , B03C1/288 , B03C2201/18 , B03C2201/26 , C07K1/145 , C07K1/22 , C12N15/1006 , Y10T436/25375 , Y10T436/2575
摘要： A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.
摘要翻译： 提供了一种用于促进从生物样品中提取级分的装置和方法。 生物样品包括非期望的材料和分数结合的固相底物。 该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。 输出区在其中接收试剂。 力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。 力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。
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公开(公告)号：US20140065654A1
公开(公告)日：2014-03-06
申请号：US13604192
申请日：2012-09-05
CPC分类号： G01N1/34 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/087 , B01L2400/043 , G01N1/4077 , G01N35/0098
摘要： A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a second zone for receiving an isolation fluid therein. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone and a second position adjacent the isolation zone. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the isolation zone in response to the force moving from the first position to the second position.
摘要翻译： 提供了用于分离生物样品中的级分的装置和方法。 该馏分与固相底物结合以限定分馏结合的固相底物。 该装置包括用于在其中接收生物样品的输入区和用于在其中接收隔离流体的第二区。 提供通常垂直于重力的力。 该力可在邻近输入区的第一位置与邻近隔离区的第二位置之间移动。 该力捕获分馏结合的固相底物，并且部分结合的固相基板响应于从第一位置移动到第二位置的力而从输入区移动到隔离区。
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