公开(公告)号：US08003777B2

公开(公告)日：2011-08-23

申请号：US12012813

申请日：2008-02-05

申请人： Donald J. Merlo ,  Dong Liu ,  Stephanie L. Burton ,  Todd P. Glancy

发明人： Donald J. Merlo ,  Dong Liu ,  Stephanie L. Burton ,  Todd P. Glancy

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N15/113 ,  C12N15/63

CPC分类号： C12N15/8216 ,  C07H21/04

摘要： The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

公开(公告)号：US09127289B2

公开(公告)日：2015-09-08

申请号：US12951813

申请日：2010-11-22

申请人： Terry R. Wright ,  Justin M. Lira ,  Donald J. Merlo ,  Nicole L. Arnold

发明人： Terry R. Wright ,  Justin M. Lira ,  Donald J. Merlo ,  Nicole L. Arnold

IPC分类号： C12N15/82 ,  C12N9/02

CPC分类号： C12N15/8274 ,  C12N9/0069 ,  C12N15/8209

摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

摘要翻译： 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

公开(公告)号：US20110107455A1

公开(公告)日：2011-05-05

申请号：US12517906

申请日：2007-12-07

申请人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

发明人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

IPC分类号： C12N15/82 ,  C12N5/10 ,  C12N15/63 ,  C12Q1/04 ,  A01H5/10 ,  A01H1/00 ,  C12N9/10 ,  C12N1/21 ,  A01N57/18 ,  C12Q1/68 ,  A01H5/00

摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

摘要翻译： 本发明涉及本文称为DSM-2的新基因。 该基因在天竺葵A3中鉴定。 DSM-2蛋白与PAT和BAR密切相关。 本发明还提供编码DSM-2蛋白质的植物优化基因，DSM-2可用作转基因性状，以将植物和植物细胞中的耐受性赋予除草剂草铵膦和双丙二酸。 主题基因的一个优选用途是选择标记。 在细菌系统中使用该基因作为选择标记可以提高植物转化的效率。 使用DSM-2作为唯一选择标记，在克隆过程中不需要额外的药用抗生素标记（如氨苄青霉素抗性）。 根据本发明，各种其他用途也是可能的。

公开(公告)号：US20090093366A1

公开(公告)日：2009-04-09

申请号：US11587893

申请日：2005-05-02

申请人： Terry R. Wright ,  Justin M. Lira ,  Donald J. Merlo ,  Nicole L. Arnold

发明人： Terry R. Wright ,  Justin M. Lira ,  Donald J. Merlo ,  Nicole L. Arnold

IPC分类号： A01N39/02 ,  C12N5/10 ,  A01H5/00 ,  A01N37/00 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N15/8274 ,  C12N9/0069 ,  C12N15/8209

摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

摘要翻译： 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

公开(公告)号：US6143526A

公开(公告)日：2000-11-07

申请号：US036987

申请日：1998-03-09

申请人： Richard H. Baltz ,  M. Christine Broughton ,  Kathryn P. Crawford ,  Krishnamurthy Madduri ,  Donald J. Merlo ,  Patti J. Treadway ,  Jan R. Turner ,  Clive Waldron

发明人： Richard H. Baltz ,  M. Christine Broughton ,  Kathryn P. Crawford ,  Krishnamurthy Madduri ,  Donald J. Merlo ,  Patti J. Treadway ,  Jan R. Turner ,  Clive Waldron

IPC分类号： C12N15/09 ,  C07K14/195 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/52 ,  C12P19/62 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/14 ,  C12N9/00

CPC分类号： C12N15/52 ,  C07K14/195 ,  C12P19/62

摘要： Spinosyn biosynthetic genes, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms.

摘要翻译： Spinosyn生物合成基因，用生物合成基因转化的多杀菌素微生物，使用生物合成基因增加多杀菌素杀虫大环内酯类的生产的方法，以及使用其基因或片段改变由产生多杀菌素的微生物产生的产物的方法。

公开(公告)号：US5567862A

公开(公告)日：1996-10-22

申请号：US369839

申请日：1995-01-06

申请人： Michael J. Adang ,  Thomas A. Rocheleau ,  Donald J. Merlo ,  Elizabeth E. Murray

发明人： Michael J. Adang ,  Thomas A. Rocheleau ,  Donald J. Merlo ,  Elizabeth E. Murray

IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  A01H4/00 ,  C12N5/14

CPC分类号： C07K14/325 ,  C12N15/8216 ,  C12N15/8286

摘要： Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.

公开(公告)号：US09617551B2

公开(公告)日：2017-04-11

申请号：US13812469

申请日：2011-07-29

申请人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

发明人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

IPC分类号： C12N15/82 ,  C12N15/74

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

公开(公告)号：US09434773B2

公开(公告)日：2016-09-06

申请号：US13617207

申请日：2012-09-14

申请人： Avutu S. Reddy ,  Sun Yuejin ,  Donald J. Merlo ,  Weiting W. Ni ,  Dayakar Pareddy ,  Zhifang Gao ,  Gregory A. Bradfisch

发明人： Avutu S. Reddy ,  Sun Yuejin ,  Donald J. Merlo ,  Weiting W. Ni ,  Dayakar Pareddy ,  Zhifang Gao ,  Gregory A. Bradfisch

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N9/00 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N9/93 ,  C12N15/8273 ,  C12Y603/02019

摘要： This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.

摘要翻译： 本发明一般涉及通过增加或产生植物中RING蛋白的一种或多种活性而与相应的非转化野生型植物细胞相比，优选在瞬时和重复的非生物胁迫条件下具有增加的产量的植物细胞。

公开(公告)号：US09427003B2

公开(公告)日：2016-08-30

申请号：US13447836

申请日：2012-04-16

申请人： Ignacio M. Larrinua ,  Donald J. Merlo ,  Avutu S. Reddy ,  Arvind Kumar ThirumalaiswamySekhar ,  Aaron T. Woosley

发明人： Ignacio M. Larrinua ,  Donald J. Merlo ,  Avutu S. Reddy ,  Arvind Kumar ThirumalaiswamySekhar ,  Aaron T. Woosley

IPC分类号： C12N15/11 ,  C12N15/63 ,  A01H5/00 ,  A23L1/10 ,  A23D9/00 ,  A23L1/20 ,  A23L1/214 ,  C07K14/325 ,  C07K14/435 ,  C11B1/00 ,  C12N9/08 ,  C12N9/02 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A23D9/00 ,  A23L7/10 ,  A23L7/198 ,  A23L11/00 ,  A23L11/05 ,  A23L19/10 ,  C07K14/195 ,  C07K14/325 ,  C07K14/37 ,  C07K14/38 ,  C07K14/415 ,  C07K14/43595 ,  C11B1/00 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0083 ,  C12Y111/01015 ,  C12Y113/00 ,  Y02A40/162

摘要： The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

摘要翻译： 本发明提供编码感兴趣的蛋白质的合成核酸序列，其特别适于在植物中表达良好。 所要求的合成序列利用植物优化的密码子，其平均来说在天然存在于植物物种中的基因中使用大致相同的频率。 本发明还包括用于除草剂耐受性，水和/或热胁迫耐受性，健康油改性和用于转化标记基因和选择标记基因的合成DNA序列。 教导含有合成序列的DNA构建体和转基因植物，以及在农业中使用植物的方法和组合物。

公开(公告)号：US20090221501A1

公开(公告)日：2009-09-03

申请号：US12371825

申请日：2009-02-16

申请人： Timothy D. Hey ,  Amanda D. Schleper ,  Scott A. Bevan ,  Scott B. Bintrim ,  Jon C. Mitchell ,  Ze Sheng Li ,  Weiting Ni ,  Baolong Zhu ,  Donald J. Merlo ,  Patricia C. Apel-Birkhold ,  Thomas Meade

发明人： Timothy D. Hey ,  Amanda D. Schleper ,  Scott A. Bevan ,  Scott B. Bintrim ,  Jon C. Mitchell ,  Ze Sheng Li ,  Weiting Ni ,  Baolong Zhu ,  Donald J. Merlo ,  Patricia C. Apel-Birkhold ,  Thomas Meade

IPC分类号： A61K38/16 ,  A01P7/04

CPC分类号： C07K14/195 ,  A01N37/46 ,  A01N63/02 ,  C07K14/24 ,  C12N15/8286 ,  Y02A40/162

摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

摘要翻译： 本发明涉及令​​人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。 在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强 来源于毒素的不同属。 本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。 最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。 （如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件 一个转基因植物，以实现更广泛的目标害虫的有效控制。 异源TC蛋白的某些优选组合也在本文中公开。 受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。