公开(公告)号：US08520213B2

公开(公告)日：2013-08-27

申请号：US13342350

申请日：2012-01-03

申请人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

发明人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

IPC分类号： G01B9/02 ,  G01J3/45

CPC分类号： G01N15/1434 ,  G01N15/0211 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1454 ,  G02B21/0056 ,  G02B21/008 ,  G03H2001/0491 ,  G03H2001/2244 ,  Y10S977/881

摘要： Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.

公开(公告)号：US20120105858A1

公开(公告)日：2012-05-03

申请号：US13342350

申请日：2012-01-03

申请人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

发明人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

IPC分类号： G01B9/02 ,  G02F1/01 ,  B82Y20/00

CPC分类号： G01N15/1434 ,  G01N15/0211 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1454 ,  G02B21/0056 ,  G02B21/008 ,  G03H2001/0491 ,  G03H2001/2244 ,  Y10S977/881

摘要： Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.

摘要翻译： 通过透明样品绘制定量相位图的方法和装置。 结合物镜，傅立叶光学元件和可编程的二维相位调制器采用宽带源，以获得图像平面中的幅度和相位信息。 称为傅里叶变换光散射（FTLS）的方法测量样品的角散射光谱。 FTLS结合光学显微镜和光散射来研究不均匀和动态的介质。 FTLS依赖于量化与相干图像场相关联的光学相位和振幅，并将其数值传播到散射平面。 仅由显微镜物镜限制的全角度信息是从极微弱的散射体，如单个微米尺寸的粒子获得的。 流式细胞仪可采用FTLS分选。

公开(公告)号：US20090290156A1

公开(公告)日：2009-11-26

申请号：US12454660

申请日：2009-05-21

申请人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

发明人： Gabriel Popescu ,  Huafeng Ding ,  Zhuo Wang

IPC分类号： G01N21/53 ,  G02F1/01

CPC分类号： G01N15/1434 ,  G01N15/0211 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1454 ,  G02B21/0056 ,  G02B21/008 ,  G03H2001/0491 ,  G03H2001/2244 ,  Y10S977/881

摘要： Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.

摘要翻译： 通过透明样品绘制定量相位图的方法和装置。 结合物镜，傅立叶光学元件和可编程的二维相位调制器采用宽带源，以获得图像平面中的幅度和相位信息。 称为傅里叶变换光散射（FTLS）的方法测量样品的角散射光谱。 FTLS结合光学显微镜和光散射来研究不均匀和动态的介质。 FTLS依赖于量化与相干图像场相关联的光学相位和振幅，并将其数值传播到散射平面。 仅由显微镜物镜限制的全角度信息是从极微弱的散射体，如单个微米尺寸的粒子获得的。 流式细胞仪可采用FTLS分选。

公开(公告)号：US07940399B2

公开(公告)日：2011-05-10

申请号：US12460808

申请日：2009-07-24

申请人： Gabriel Popescu ,  Zhuo Wang

发明人： Gabriel Popescu ,  Zhuo Wang

IPC分类号： G01B11/02

CPC分类号： G01B9/02083 ,  G01B9/02024 ,  G01B2290/70 ,  G01N21/21 ,  G01N21/23 ,  G02B21/0092 ,  G02B21/18

摘要： Methods for displaying anisotropic properties of an object. The object is illuminated with a first test beam characterized by a first polarization that, after traversing the object, is combined with a reference beam. The combined light of the first transmitted test beam and the reference beam is analyzed by a first pair of polarization analyzers, and interference created between the first transmitted test beam and the reference beam as analyzed by the first pair of analyzers is detected to derive intensity, phase and polarization of the first transmitted test beam. The same is then done with a second test beam that has a polarization with a component orthogonal to the first polarization. Based on the two analyzed beams, complex elements of a Jones matrix associated with the object in a local coordinate system are determined and a plurality of tangible images are displayed that characterize the object based on the complex elements of the Jones matrix.

摘要翻译： 显示对象各向异性的方法。 物体被第一测试光束照亮，第一测试光束的特征在于第一极化，在穿过物体之后，与参考光束组合。 通过第一对偏振分析器分析第一透射测试光束和参考光束的组合光，并且检测由第一对分析器分析的在第一透射测试光束和参考光束之间产生的干涉，以导出强度， 第一个发射的测试光束的相位和极化。 然后用具有与第一极化正交的分量的偏振的第二测试光束进行相同的操作。 基于两个分析的波束，确定与局部坐标系中的对象相关联的Jones矩阵的复数元素，并且基于Jones矩阵的复数元素来显示表征对象的多个有形图像。

公开(公告)号：US20100027027A1

公开(公告)日：2010-02-04

申请号：US12460808

申请日：2009-07-24

申请人： Gabriel Popescu ,  Zhuo Wang

发明人： Gabriel Popescu ,  Zhuo Wang

IPC分类号： G01B9/02

CPC分类号： G01B9/02083 ,  G01B9/02024 ,  G01B2290/70 ,  G01N21/21 ,  G01N21/23 ,  G02B21/0092 ,  G02B21/18

摘要： Methods for displaying anisotropic properties of an object. The object is illuminated with a first test beam characterized by a first polarization that, after traversing the object, is combined with a reference beam. The combined light of the first transmitted test beam and the reference beam is analyzed by a first pair of polarization analyzers, and interference created between the first transmitted test beam and the reference beam as analyzed by the first pair of analyzers is detected to derive intensity, phase and polarization of the first transmitted test beam. The same is then done with a second test beam that has a polarization with a component orthogonal to the first polarization. Based on the two analyzed beams, complex elements of a Jones matrix associated with the object in a local coordinate system are determined and a plurality of tangible images are displayed that characterize the object based on the complex elements of the Jones matrix.

摘要翻译： 显示对象各向异性的方法。 物体被第一测试光束照亮，第一测试光束的特征在于第一极化，在穿过物体之后，与参考光束组合。 通过第一对偏振分析器分析第一透射测试光束和参考光束的组合光，并且检测由第一对分析器分析的在第一透射测试光束和参考光束之间产生的干涉，以导出强度， 第一个发射的测试光束的相位和极化。 然后用具有与第一极化正交的分量的偏振的第二测试光束进行相同的操作。 基于两个分析的波束，确定与局部坐标系中的对象相关联的Jones矩阵的复数元素，并且基于Jones矩阵的复数元素来显示表征对象的多个有形图像。

公开(公告)号：US08184298B2

公开(公告)日：2012-05-22

申请号：US12454660

申请日：2009-05-21

申请人： Gabriel Popescu ,  Zhuo Wang

发明人： Gabriel Popescu ,  Zhuo Wang

IPC分类号： G01B9/02 ,  G01J3/45 ,  G02B21/00

CPC分类号： G01N15/1434 ,  G01N15/0211 ,  G01N15/1459 ,  G01N2015/0038 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1454 ,  G02B21/0056 ,  G02B21/008 ,  G03H2001/0491 ,  G03H2001/2244 ,  Y10S977/881

摘要： Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.

摘要翻译： 通过透明样品绘制定量相位图的方法和装置。 结合物镜，傅立叶光学元件和可编程的二维相位调制器采用宽带源，以获得图像平面中的幅度和相位信息。 称为傅里叶变换光散射（FTLS）的方法测量样品的角散射光谱。 FTLS结合光学显微镜和光散射来研究不均匀和动态的介质。 FTLS依赖于量化与相干图像场相关联的光学相位和振幅，并将其数值传播到散射平面。 仅由显微镜物镜限制的全角度信息是从极微弱的散射体，如单个微米尺寸的粒子获得的。 流式细胞仪可采用FTLS分选。

公开(公告)号：US07557929B2

公开(公告)日：2009-07-07

申请号：US10871610

申请日：2004-06-18

申请人： Christopher M. Fang-Yen ,  Gabriel Popescu ,  Changhuei Yang ,  Adam Wax ,  Ramachandra R. Dasari ,  Michael S. Feld

发明人： Christopher M. Fang-Yen ,  Gabriel Popescu ,  Changhuei Yang ,  Adam Wax ,  Ramachandra R. Dasari ,  Michael S. Feld

IPC分类号： G01B9/02 ,  G01B11/02

CPC分类号： G01B9/02091 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/7232 ,  G01B9/02002 ,  G01B9/02007 ,  G01B9/02011 ,  G01B9/02057 ,  G01B9/02063 ,  G01B9/02067 ,  G01B9/02069 ,  G01B9/02071 ,  G01B9/02072 ,  G01B9/02078 ,  G01B9/02083 ,  G01B9/0209 ,  G01B2290/45 ,  G01B2290/60 ,  G01B2290/70 ,  G01J9/04 ,  G01N21/45

摘要： Preferred embodiments of the present invention are directed to systems for phase measurement which address the problem of phase noise using combinations of a number of strategies including, but not limited to, common-path interferometry, phase referencing, active stabilization and differential measurement. Embodiment are directed to optical devices for imaging small biological objects with light. These embodiments can be applied to the fields of, for example, cellular physiology and neuroscience. These preferred embodiments are based on principles of phase measurements and imaging technologies. The scientific motivation for using phase measurements and imaging technologies is derived from, for example, cellular biology at the sub-micron level which can include, without limitation, imaging origins of dysplasia, cellular communication, neuronal transmission and implementation of the genetic code. The structure and dynamics of sub-cellular constituents cannot be currently studied in their native state using the existing methods and technologies including, for example, x-ray and neutron scattering. In contrast, light based techniques with nanometer resolution enable the cellular machinery to be studied in its native state. Thus, preferred embodiments of the present invention include systems based on principles of interferometry and/or phase measurements and are used to study cellular physiology. These systems include principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) wherein interference within the cellular components themselves is used, or in the alternative the principles of LCI and LSS can be combined to result in systems of the present invention.

摘要翻译： 本发明的优选实施例涉及用于相位测量的系统，其使用多种策略的组合来解决相位噪声问题，包括但不限于公共路径干涉测量，相位参考，主动稳定和差分测量。 实施例涉及用于用光成像小生物物体的光学装置。 这些实施方案可以应用于例如细胞生理学和神经科学领域。 这些优选实施例基于相位测量和成像技术的原理。 使用相位测量和成像技术的科学动机源于例如亚微米级别的细胞生物学，其可以包括但不限于成像发育异常起源，细胞通讯，神经元传播和遗传密码的实现。 亚细胞组分的结构和动力学目前不能使用现有的方法和技术（例如x射线和中子散射）在其天然状态下研究。 相比之下，具有纳米分辨率的基于光的技术使得能够以其天然状态研究细胞机械。 因此，本发明的优选实施例包括基于干涉测量和/或相位测量的原理的系统，并且用于研究细胞生理学。 这些系统包括使用光学干涉仪来测量相位的低相干干涉测量（LCI）原理，或使用其中使用细胞部件本身内部的干扰的光散射光谱（LSS），或者替代地，LCI和LSS的原理可以组合以产生 在本发明的系统中。

公开(公告)号：US08772693B2

公开(公告)日：2014-07-08

申请号：US11389670

申请日：2006-03-24

申请人： Gabriel Popescu ,  Ramachandra Dasari ,  Takahiro Ikeda

发明人： Gabriel Popescu ,  Ramachandra Dasari ,  Michael Feld ,  Takahiro Ikeda

IPC分类号： H01L27/00

CPC分类号： H01L27/14601 ,  G01N15/10 ,  G01N15/1475 ,  G01N21/453 ,  G01N33/49 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/145 ,  G01N2015/1454 ,  G01N2015/1497 ,  G06K9/00134

摘要： Hilbert phase microscopy (HPM) as an optical technique for measuring high transverse resolution quantitative phase images associated with optically transparent objects. Due to its single-shot nature, HPM is suitable for investigating rapid phenomena that take place in transparent structures such as biological cells. A preferred embodiment is used for measuring biological systems including measurements on red blood cells, while its ability to quantify dynamic processes on the millisecond scale, for example, can be illustrated with measurements on evaporating micron-size water droplets.

摘要翻译： 希尔伯特相位显微镜（HPM）作为用于测量与光学透明物体相关的高横向分辨率定量相位图像的光学技术。 由于其单次性质，HPM适用于调查在诸如生物细胞的透明结构中发生的快速现象。 优选的实施例用于测量生物系统，包括对红血细胞的测量，而其量化毫秒级别的动态过程的能力可以用蒸发微米级水滴的测量来说明。

公开(公告)号：US20120193494A1

公开(公告)日：2012-08-02

申请号：US13385976

申请日：2012-03-19

申请人： Gabriel Popescu

发明人： Gabriel Popescu

IPC分类号： F16B2/12

CPC分类号： B25B5/02 ,  B25B5/101 ,  B25B5/14

摘要： A telescopic pipe clamp designed to fastening short or long work pieces with the same tool.The clamp includes: Two pipes. The first one carries a fixed jaw. The second one, which has the interior dimensions slight larger than first pipe in order to can slide along it, carries a movable jaw. Both pipes have the holes drilled at the same alignment. A pin to be inserted through above mentioned holes in order to secure the pipes together.

摘要翻译： 一个伸缩管夹，用于用相同的工具紧固短或长的工件。 夹具包括：两根管。 第一个携带固定的颚。 第二个，其内部尺寸略大于第一管，以便沿其滑动，携带可动夹爪。 两个管道都有相同对准钻孔。 通过上述孔插入的销钉，以将管道固定在一起。

公开(公告)号：US06525823B1

公开(公告)日：2003-02-25

申请号：US09691300

申请日：2000-10-18

申请人： Aristide Dogariu ,  Gabriel Popescu

发明人： Aristide Dogariu ,  Gabriel Popescu

IPC分类号： G01B902

CPC分类号： G01N15/0211 ,  G01N9/32 ,  G01N2015/0092 ,  G01N2015/0693

摘要： An optical system for monitoring a colloidal suspension includes a chamber positioned to receive a sample of the suspension while the suspension is circulating in a mechanical system. An interferometer is positioned to generate interference patterns developed by a combination of a reference radiation signal with a sample radiation signal indicative of a characteristic in the suspension.

摘要翻译： 用于监测胶体悬浮液的光学系统包括定位成在悬架在机械系统中循环时接收悬架样品的腔室。 定位干涉仪以产生由参考辐射信号与指示悬浮液中的特征的样本辐射信号的组合而产生的干涉图案。