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    METHODS OF MAKING ANTIBODIES
    1.
    发明申请
    METHODS OF MAKING ANTIBODIES 有权

    公开(公告)号：US20220056134A1

    公开(公告)日：2022-02-24

    申请号：US17454015

    申请日：2021-11-08

    申请人： Genentech, Inc.

    发明人： Kamal Kishore JOSHI ,  Paul J. Carter ,  Yiyuan Yin

    IPC分类号： C07K16/28 ,  C12N5/10

    摘要： Provided are, inter alia, methods of improving pairing of a heavy chain and a light chain of an antibody (such as a bispecific antibody). Also provided are antibodies (e.g., bispecific antibodies) generated using such methods, libraries, and methods of screening such libraries.

    Method for making multispecific antibodies having heteromultimeric and common components
    6.
    发明授权
    Method for making multispecific antibodies having heteromultimeric and common components 有权
    制备具有异源多聚体和共同成分的多特异性抗体的方法

    公开(公告)号：US09409989B2

    公开(公告)日：2016-08-09

    申请号：US14142663

    申请日：2013-12-27

    申请人： GENENTECH, INC.

    发明人： W. Robert Arathoon ,  Paul J. Carter ,  Anne M. Merchant ,  Leonard G. Presta

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N5/10 ,  C12N15/09 ,  C12N15/13 ,  C12N15/63 ,  C07K16/46 ,  C07K16/28 ,  C07K1/22 ,  A61K38/00

    CPC分类号： C07K16/2863 ,  A61K38/00 ,  C07K1/22 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/46 ,  C07K16/468 ,  C07K2317/732 ,  Y10S435/972

    摘要： The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.

    摘要翻译： 本发明涉及制备异源多聚体多肽如双特异性抗体，双特异性免疫粘附素和抗体免疫粘附素嵌合体的方法。 本发明还涉及使用该方法制备的异源多聚体。 通常，该方法提供具有与具有抗体结合结构域的每个异聚多肽相关联的共同轻链的多特异性抗体。 另外，该方法还包括将多特异性抗体引入第一多肽的界面处的第二多肽的界面上的特异性和互补相互作用，以促进异源多聚体形成并阻碍同源多聚体形成; 和/或在第二多肽的界面处的第一多肽和相应的游离含硫醇残基的界面处的游离含硫醇残基，使得在第一和第二多肽之间形成非天然存在的二硫键。 该方法允许相对于不想要的异多元反应物和同源多聚体增强所需异源多聚体的形成。