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    Preparation of heterologous proteins on oil bodies
    9.
    发明授权
    Preparation of heterologous proteins on oil bodies 失效
    在油体上制备异源蛋白质

    公开(公告)号：US06753167B2

    公开(公告)日：2004-06-22

    申请号：US09893525

    申请日：2001-06-29

    申请人： Maurice M. Moloney ,  Gijs van Rooijen

    发明人： Maurice M. Moloney ,  Gijs van Rooijen

    IPC分类号： C12N1562

    CPC分类号： C12N9/6483 ,  C07K14/415 ,  C07K14/545 ,  C07K14/61 ,  C07K14/8139 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/6429 ,  C12N15/8222 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y304/21005 ,  C12Y304/23004

    摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

    摘要翻译： 本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。 这些特征的发现允许本发明用于生产重组蛋白质的方法，其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。 本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体，特别是植物种子中表达目标多肽的独特特征的方法。 所述多肽可以包括但不限于：种子储存蛋白，酶，生物活性肽，抗体等。 还可以修饰本发明以从非植物宿主细胞中回收与油体蛋白融合的重组多肽。 另外，本发明提供了一种使用与种子发芽期间释放的种子油体相关的重组蛋白的方法，用于表达能够保护来自病原体的幼苗的多肽。 最后，油体蛋白与油体的持续结合可以进一步利用，以开发生物学手段来产生可用于生物转化底物的新型固定化酶。