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    Production method and preparation method of glucans
    2.
    发明授权
    Production method and preparation method of glucans 有权
    葡聚糖的制备方法和制备方法

    公开(公告)号：US07229801B2

    公开(公告)日：2007-06-12

    申请号：US10475943

    申请日：2002-05-27

    申请人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

    发明人： Kazutoshi Fujii ,  Yoshinobu Terada ,  Michiyo Yanase ,  Koji Odan ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki ,  Shigetaka Okada

    IPC分类号： C12P19/60 ,  C12N9/10

    CPC分类号： C08B37/0009 ,  C08B30/20 ,  C08B33/00 ,  C12P19/18

    摘要： A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.

    摘要翻译： 制备葡聚糖的第一种方法包括使含有蔗糖，底漆，无机磷酸盐或葡萄糖-1-磷酸，蔗糖磷酸化酶和葡聚糖磷酸化酶的反应溶液反应生成葡聚糖的步骤。 从反应开始到反应结束的反应溶液的蔗糖磷酸酯比例的最大值不超过约17。第二种葡聚糖的制备方法包括以下步骤：使含有蔗糖的反应溶液， 底物，无机磷酸盐或葡萄糖-1-磷酸盐，蔗糖磷酸化酶和葡聚糖磷酸化酶反应产生葡聚糖。 反应在约40℃至约70℃的温度下进行。

    Electronic control unit
    3.
    发明授权
    Electronic control unit 有权
    电子控制单元

    公开(公告)号：US06195263B1

    公开(公告)日：2001-02-27

    申请号：US09271167

    申请日：1999-03-18

    申请人： Koji Aoike ,  Naotaka Murakami ,  Hiroki Takata ,  Osamu Yamato

    发明人： Koji Aoike ,  Naotaka Murakami ,  Hiroki Takata ,  Osamu Yamato

    IPC分类号： H05K700

    CPC分类号： H05K5/0069 ,  H01L2224/06153 ,  H01L2224/45124 ,  H01L2224/48091 ,  H01L2224/48472 ,  H01L2224/4943 ,  H01L2924/01068 ,  H01R12/57 ,  Y10S439/949 ,  Y10T29/49172 ,  H01L2924/00014 ,  H01L2924/00

    摘要： In an electronic control unit, a pad portion thereof is firmly fixed to a terminal plate and vibration of the terminal plate under supersonic vibration is prevented so as to secure the bonding strength between the terminal plate and the wire during wire bonding. One lead portion of each terminal plate is connected to a pin of the connector and another lead portion is wire bonded to the terminal of the circuit substrate. The leads have bent portions extending from an exposed surface to be bonded in opposite directions to be embedded in the pad portion of the case such that the lead portion of the terminal plate is firmly fixed to the case.

    摘要翻译： 在电子控制单元中，其焊盘部牢固地固定在端子板上，防止了超音速振动下端子板的振动，从而确保了引线接合时端子板与导线之间的接合强度。 每个端子板的一个引线部分连接到连接器的引脚，另一个引线部分引线接合到电路基板的端子。 引线具有从暴露表面延伸的弯曲部分，以相对方向接合以嵌入壳体的焊盘部分，使得端子板的引线部分牢固地固定在壳体上。

    Method of heat-stabilizing α-glucan phosphorylase (GP)
    6.
    发明授权
    Method of heat-stabilizing α-glucan phosphorylase (GP) 有权
    热稳定α-葡聚糖磷酸化酶（GP）的方法

    公开(公告)号：US07723090B2

    公开(公告)日：2010-05-25

    申请号：US12465135

    申请日：2009-05-13

    申请人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Michiyo Yanase ,  Hiroki Takata ,  Kazutoshi Fujii ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C07H21/04 ,  C12N9/12

    CPC分类号： C12N9/1051 ,  C12P19/02 ,  C12P19/04

    摘要： An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating.

    摘要翻译： 提供通过改性天然α-葡聚糖磷酸化酶获得的具有改进的热稳定性的α-葡聚糖磷酸化酶，以及制备具有改善的热稳定性的该α-葡聚糖磷酸化酶的方法。 天然α-葡聚糖磷酸化酶衍生自植物，具有改善的热稳定性的该α-葡聚糖磷酸化酶具有与天然α-葡聚糖磷酸化酶不同的氨基酸残基，所述氨基酸残基至少一个位置选自位置 对应于基序序列1L或1H中的位置4，对应于基序序列2中的位置4的位置和对应于基序序列3L或3H中的位置7的位置，并且其中具有α-葡聚糖磷酸化酶的酶活性 在60℃下在20mM柠檬酸盐缓冲液（pH7.7）中加热10分钟后，在37℃下改善的热稳定性为具有改善的热稳定性在37℃的α-葡聚糖磷酸化酶的酶活性的20％或更多 C.加热前。

    Method of producing glycogen
    7.
    发明授权
    Method of producing glycogen 有权
    糖原的制备方法

    公开(公告)号：US07670812B2

    公开(公告)日：2010-03-02

    申请号：US11575794

    申请日：2005-09-28

    申请人： Hideki Kajiura ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Hideki Kajiura ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C12P19/04 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P19/04 ,  C12N9/2411 ,  C12P19/18

    摘要： A method of producing glycogen comprising a step of: allowing a branching enzyme having the ability to synthesize glycogen to act on a substrate in a solution to produce a glycogen, wherein the substrate is an α-glucan being linked mainly with α-1,4-glucosidic bonds and having a degree of polymerization of 4 or more, and the number-average molecular weight of saccharides in the solution before initiation of the reaction is more than 180 but not more than 150,000. (The branching enzyme activity of the branching enzyme)/(the molecular-weight-decreasing activity of the branching enzyme) can be 500 or less. The branching enzyme can be a thermostable branching enzyme.

    摘要翻译： 一种制备糖原的方法，包括以下步骤：使具有合成糖原的能力的分支酶作用于溶液中的底物以产生糖原，其中所述底物是α-葡聚糖主要与α-1,4 - 糖苷键，聚合度为4以上，反应开始前溶液中的糖类的数均分子量大于180，但不大于150,000。 （支化酶的分支酶活性）/（分支酶的分子量降低活性）可以为500以下。 分支酶可以是热稳定分支酶。

    Method Of Producing Glycogen
    8.
    发明申请
    Method Of Producing Glycogen 有权
    生产糖原的方法

    公开(公告)号：US20080131941A1

    公开(公告)日：2008-06-05

    申请号：US11575794

    申请日：2005-09-28

    申请人： Hideki Kajiura ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    发明人： Hideki Kajiura ,  Hiroki Takata ,  Takeshi Takaha ,  Takashi Kuriki

    IPC分类号： C12P19/04

    CPC分类号： C12P19/04 ,  C12N9/2411 ,  C12P19/18

    摘要： A method of producing glycogen comprising a step of: allowing a branching enzyme having the ability to synthesize glycogen to act on a substrate in a solution to produce a glycogen, wherein the substrate is an α-glucan being linked mainly with α-1,4-glucosidic bonds and having a degree of polymerization of 4 or more, and the number-average molecular weight of saccharides in the solution before initiation of the reaction is more than 180 but not more than 150,000. (The branching enzyme activity of the branching enzyme)/(the molecular-weight-decreasing activity of the branching enzyme) can be 500 or less. The branching enzyme can be a thermostable branching enzyme.

    摘要翻译： 一种制备糖原的方法，包括以下步骤：使具有合成糖原的能力的分支酶在溶液中作用于底物以产生糖原，其中所述底物是主要与α-1,4连接的α-葡聚糖 - 糖苷键，聚合度为4以上，反应开始前溶液中的糖类的数均分子量大于180，但不大于150,000。 （支化酶的分支酶活性）/（分支酶的分子量降低活性）可以为500以下。 分支酶可以是热稳定分支酶。

    Electronic component
    9.
    发明授权
    Electronic component 有权
    电子元器件

    公开(公告)号：US06302706B1

    公开(公告)日：2001-10-16

    申请号：US09475213

    申请日：1999-12-29

    申请人： Kouji Aokie ,  Naotaka Murakami ,  Hiroki Takata ,  Osamu Yamato ,  Katsuhiko Onoda

    发明人： Kouji Aokie ,  Naotaka Murakami ,  Hiroki Takata ,  Osamu Yamato ,  Katsuhiko Onoda

    IPC分类号： H01R1200

    CPC分类号： H01R13/405 ,  H01L2224/05554 ,  H01L2224/45124 ,  H01L2224/48472 ,  H01L2224/49175 ,  H01R13/6658 ,  H01L2924/00

    摘要： In order to precisely detect positions of respective lead portions of an electronic component and to reduce the cost of production of the electronic component, the electronic component has a resinous pad portion and a plurality of terminal units embedded in the pad portion. Each of the terminal units is provided with lead portions that are disposed parallel to one another with their terminal faces exposed, and junction portions for junction between bonding wires and the lead portions are formed on the terminal faces. Corner portions are formed at predetermined sections on the terminal faces. In this case, even if resin of the pad portion has adhered to the peripheries of the terminal faces and fouled the terminal faces, since the lead portions have the corner portions, images of the lead portions can be detected precisely. Accordingly, the operation of connecting the bonding wires to the terminal faces is performed more efficiently, whereby the cost of production of the electronic component can be reduced.

    摘要翻译： 为了精确地检测电子部件的各个引线部的位置，并且为了降低电子部件的制造成本，电子部件具有嵌入焊盘部的树脂焊盘部和多个端子部。 每个端子单元设置有彼此平行布置的引线部分，其端子面暴露，并且在端子面上形成用于接合线和引线部分之间的接合部的接合部分。 拐角部分形成在终端面上的预定部分。 在这种情况下，即使焊盘部分的树脂粘附到端子表面的周边并且使端子端部被污染，由于引线部分具有拐角部分，因此可以精确地检测引线部分的图像。 因此，更有效地进行将接合线连接到端子面的动作，能够降低电子部件的制造成本。