公开(公告)号：US08454820B2

公开(公告)日：2013-06-04

申请号：US12872735

申请日：2010-08-31

申请人： Hiroshi Aoki ,  Hiroaki Tao ,  Akiko Kitajima

发明人： Hiroshi Aoki ,  Hiroaki Tao ,  Akiko Kitajima

IPC分类号： G01N27/327

CPC分类号： G01N33/542 ,  G01N2458/30

摘要： Constitute a molecular recognition probe comprising: an electrochemically active group; an activity suppression group that suppresses an electrochemical activity of the electrochemically active group; a receptor area where a molecule of a target substance is specifically recognized; and a molecule area where a steric structure is changed as a result of molecular recognition; wherein the electrochemically active group is suppressed of its activity by the activity suppression group before the molecule is recognized and restores its activity after the molecule is recognized; or constitute a molecular recognition sensor by providing an anchor area on the molecular recognition probe and fixing it on a surface of an electrode.

摘要翻译： 构成分子识别探针，其包含：电化学活性基团; 抑制电化学活性基团的电化学活性的活性抑制基团; 特异性识别目标物质分子的受体区域; 以及分子识别结果改变空间结构的分子区域; 其中所述电化学活性基团在分子被识别之前被活性抑制基团抑制其活性，并且在分子被识别后恢复其活性; 或通过在分子识别探针上提供锚定区域并将其固定在电极的表面上来构成分子识别传感器。

公开(公告)号：US20090233816A1

公开(公告)日：2009-09-17

申请号：US12385755

申请日：2009-04-17

申请人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

发明人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

IPC分类号： C40B60/14

CPC分类号： B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L9/54 ,  B01L9/547 ,  B01L2200/022 ,  B01L2300/0838 ,  G01N35/1067 ,  G01N35/1074 ,  Y10T436/2575

摘要： At each lattice point of pantographs, a capillary is held perpendicular to the pantograph lattice. The pantograph includes: rings to serve as lattice points; and shafts linking the rings with one another. Each of the capillaries is supported by the two rings. The shafts are free to rotate about an intersection. The ring is fixed to the capillary, but the ring is a movable ring being moveable freely up and down in the axial direction of the capillary in conjunction with the pantograph. The lattice distance can be determined accomplished by moving up or down the movable ring at an arbitrarily-chosen lattice point in a pantograph. Accordingly, all the capillaries move in parallel to one another, and the ends of all the capillaries always stay within a single plane.

摘要翻译： 在受电弓的每个格点处，毛细管垂直于受电弓格架。 受电弓包括：作为格点的戒指; 以及将环彼此连接的轴。 每个毛细管由两个环支撑。 轴可以绕交叉点自由旋转。 环被固定在毛细管上，但是环是可移动的环，其可以与受电弓一起在毛细管的轴向方向上上下移动。 可以通过在受电弓中以任意选择的格点向上或向下移动可移动环来确定晶格距离。 因此，所有毛细血管相互平行移动，所有毛细血管的末端始终保持在单个平面内。

公开(公告)号：US20090305910A1

公开(公告)日：2009-12-10

申请号：US12309072

申请日：2007-06-27

申请人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

发明人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

IPC分类号： C40B60/00

CPC分类号： G01N35/1067 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00659 ,  B01L3/0262 ,  B01L9/54 ,  B01L2200/022 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2400/025 ,  G01N35/1074

摘要： At each lattice point 2 of pantographs 1, a capillary 3 is held perpendicular to the pantograph lattice. The pantograph 1 includes: rings 4 and 5 to serve as lattice points; and shafts 6 linking the rings with one another. Each of the capillaries 3 is supported by the two rings 4 and 5. The shafts are free to rotate about an intersection 7. The ring 5 is fixed to the capillary 3, but the ring 4 is a movable ring being moveable freely up and down in the axial direction of the capillary 3 in conjunction with the pantograph 1. The lattice distance can be determined accomplished by moving up or down the movable ring at an arbitrarily-chosen lattice point in a pantograph 1. Accordingly, all the capillaries move in parallel to one another, and the ends of all the capillaries always stay within a single plane.

摘要翻译： 在受电弓1的每个格点2处，毛细管3垂直于受电弓格架保持。 受电弓1包括：环4和5作为格点; 以及将环彼此连接的轴6。 每个毛细管3由两个环4和5支撑。轴可以绕交叉点7自由旋转。环5固定到毛细管3上，但是环4是可上下移动的可动环 在集电弓1上与毛细管3的轴向方向一致。可以通过在受电弓1中任意选择的格点上移动上下移动环来确定晶格距离。因此，所有毛细管平行移动 所有毛细血管的末端总是保持在单一的平面内。

公开(公告)号：US08808625B2

公开(公告)日：2014-08-19

申请号：US12201552

申请日：2008-08-29

申请人： Hiroshi Aoki ,  Hiroaki Tao ,  Masaki Torimura ,  Takashi Ikeda

发明人： Hiroshi Aoki ,  Hiroaki Tao ,  Masaki Torimura ,  Takashi Ikeda

IPC分类号： G01N21/00 ,  B01L3/02 ,  G01N1/00 ,  G01N1/16 ,  G01N1/26 ,  G01N35/10 ,  B01L3/00 ,  G01N1/02 ,  G01F25/00 ,  G01N1/14

CPC分类号： B01L3/0241 ,  B01L3/0217 ,  B01L9/52 ,  B01L2200/022 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  G01N35/1011 ,  G01N2035/1039

摘要： In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.

摘要翻译： 在用于将诸如样品溶液的液体分配在诸如玻璃载体的基底上的分配装置中，分配装置包括设置有远端和近端的毛细管，泵单元构造成泵送操作液体 进入毛细管并将工作液体泵出毛细管;以及控制器，被配置为控制泵单元，以便改变毛细管中的操作液体的液面的位置，使得预定体积的液体从 进入毛细管的远端和毛细管中吸入的液体从远端排出。 结果，可以精确地分配非常小量的液体，例如纳升。

公开(公告)号：US08663577B2

公开(公告)日：2014-03-04

申请号：US12385755

申请日：2009-04-17

申请人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

发明人： Hiroshi Aoki ,  Masaki Torimura ,  Hiroaki Tao ,  Takashi Ikeda

IPC分类号： B01L3/00

CPC分类号： B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L9/54 ,  B01L9/547 ,  B01L2200/022 ,  B01L2300/0838 ,  G01N35/1067 ,  G01N35/1074 ,  Y10T436/2575

摘要： A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.

摘要翻译： 一种检测器，包括多个点样头，所述多个点样头中的每一个具有在尖端部分的排出部分，所述多个点样头形成m×n阵列（m，n> 1），其中m个点样头纵向排列 并且n个点头交叉排列; 以及变桨机构，被配置为改变沿横向方向排列的多个点样头的阵列间距和沿横向排列的多个点样头的阵列间距。

公开(公告)号：US20120034672A1

公开(公告)日：2012-02-09

申请号：US13274764

申请日：2011-10-17

申请人： SungBae KIM ,  Hiroaki Tao ,  Moritoshi Sato

发明人： SungBae KIM ,  Hiroaki Tao ,  Moritoshi Sato

IPC分类号： C12N9/02 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/53 ,  C12N15/63

CPC分类号： C12Y113/12005 ,  C12N9/0069 ,  C12Q1/66

摘要： This invention provides a genetically modified marine luciferase such as Gaussia luciferase, which has high bioluminescence intensity, and has high bioluminescence stability and/or red-shifted wavelength. Specifically disclosed is a luciferase variant with improved optical property obtained by replacing at least one amino acid residue among the amino acid sequence of a marine luciferase at positions corresponding to positions 89 to 118 in the amino acid sequence of Gaussia luciferase (GLuc), wherein an amino acid residue at a position corresponding to at least one selected from positions 89, 90, 95, 97, 100, 108, 112, 115, and 118 in the amino acid sequence of GLuc is replaced by way of conservative amino acid replacement. The above-mentioned replacement in a marine luciferase improves enzymatic activity of the luciferase. Also disclosed is a bioluminescent probe having an improved optical property, which is produced using the luciferase variant of the present invention.

摘要翻译： 本发明提供了具有高生物发光强度，高生物发光稳定性和/或红移波长的遗传修饰的海洋荧光素酶如高丝萤光素酶。 具体公开的是具有改善的光学性能的荧光素酶变体，其通过在高斯萤光素酶（GLuc）的氨基酸序列中相应于位置89至118的位置处替代海洋萤光素酶的氨基酸序列中的至少一个氨基酸残基获得，其中 在与GLuc的氨基酸序列中的位置89,90,97,97,100,108,112,115和118中的至少一个相对应的位置上的氨基酸残基被保守氨基酸置换。 上述在海洋荧光素酶中的替代改善了荧光素酶的酶活性。 还公开了具有改进的光学性质的生物发光探针，其使用本发明的荧光素酶变体产生。

公开(公告)号：US07572999B2

公开(公告)日：2009-08-11

申请号：US11184671

申请日：2005-07-19

申请人： Hiroaki Tao ,  Tetsuya Nakazato ,  Kenichi Sakata

发明人： Hiroaki Tao ,  Tetsuya Nakazato ,  Kenichi Sakata

IPC分类号： B23K10/00

CPC分类号： H01J49/105 ,  G01N30/7206 ,  H05H1/30 ,  H05H1/42

摘要： To provide an inductively-coupled plasma torch capable of realizing a GC analysis or the like and a solution analysis by the same torch without removing a capillary tube, etc. The injector tube of the inductively-coupled plasma torch includes an outer injector tube 11 for introducing an atomized solution sample or the like into the inductively-coupled plasma, and an inner injector tube 12 for introducing gaseous molecules into the inductively-coupled plasma, housed in the outer injector tube as integrated and coaxially with it. The inner injector tube is able to convey make-up gas, and has a capillary tube 4 for conveying gaseous molecules and carrier gas together. For sample introduction in analyzing a gaseous sample, the inner injector tube 12 is used, and for sample introduction in analyzing an aerosol sample, the outer injector tube 11 is used.

摘要翻译： 提供能够实现GC分析等的电感耦合等离子体焰炬和通过相同的手电筒进行溶液分析而不去除毛细管等。电感耦合等离子体焰炬的喷射管包括外部喷射管11，其用于 将雾化溶液样品等引入电感耦合等离子体，以及内部注射管12，用于将气态分子引入感应耦合等离子体中，该等离子体被容纳在与其一体并与其同轴的外部注射管中。 内部注射管能够输送补充气体，并具有用于将气态分子和载气一起输送的毛细管4。 对于分析气体样品的样品介绍，使用内部注射管12，并且为了在分析气溶胶样品时进行样品引入，使用外部注射管11。

公开(公告)号：US06550934B2

公开(公告)日：2003-04-22

申请号：US09739680

申请日：2000-12-20

申请人： Hiroaki Tao ,  Tetsuya Nakazato

发明人： Hiroaki Tao ,  Tetsuya Nakazato

IPC分类号： H01S300

CPC分类号： H01S3/092 ,  H01J61/025 ,  H01J61/30

摘要： A light emitting device having a lamp for producing light when supplied with electric current, and an elongated optical material having a portion disposed within the lamp and one end located outside the lamp and serving as an outlet of emitted light. The optical material may be (a) a light transmissive, fluorescent or phosphorescent adapted to emit fluorescent or phosphorescent light upon being excited by irradiation with the light of the lamp so that the fluorescent or phosphorescent light is transmitted to the outlet, (b) a rod transparent to the light of the lamp so that the light from the lamp entering the rod is transmitted to the outlet, or (c) a light transmissive, laser generating optical material adapted to emit a laser beam upon being excited with the light of the lamp so that the laser beam is transmitted to the outlet.

摘要翻译： 一种发光装置，其具有用于在供电时产生光的灯，以及细长的光学材料，其具有设置在灯内的部分，并且一端位于灯外部并且用作发射光的出口。 光学材料可以是（a）适于在通过用灯的光照射而激发荧光或磷光的光的透光，荧光或磷光，使得荧光或磷光被传输到出口，（b） 杆对灯的光线透明，使得来自灯的灯进入杆的光被传输到出口，或者（c）适于发射激光束的透光激光产生光学材料， 灯，使激光束传输到出口。

公开(公告)号：US06339954B1

公开(公告)日：2002-01-22

申请号：US09531430

申请日：2000-03-20

申请人： Ryuichi Naganawa ,  Kazutoshi Noda ,  Hiroaki Tao ,  Mamoru Tominaga

发明人： Ryuichi Naganawa ,  Kazutoshi Noda ,  Hiroaki Tao ,  Mamoru Tominaga

IPC分类号： G01N2900

CPC分类号： G01N29/036 ,  G01N33/0049 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/02809

摘要： In a method of analyzing a concentration of a target substance contained in a fluid using a quartz oscillator having a surface covered with a layer capable of absorbing the target substance, the temperature of the fluid and the quartz oscillator is controlled at substantially the same predetermined temperature selected according to the concentration of the target substance in said fluid, so that the analysis can be made with optimum sensitivity. A device for analyzing a concentration of a target substance contained in a fluid, includes a contacting chamber accommodating a quartz oscillator, a temperature controller, disposed in a feed passage for feeding the fluid into said contacting chamber, for adjusting a temperature of the fluid at a desired predetermined temperature; and a detector of a frequency of the quartz oscillator.

摘要翻译： 在使用具有覆盖有能够吸收目标物质的层的表面的石英振荡器来分析流体中所含的目标物质的浓度的方法中，将流体和石英振荡器的温度控制在基本相同的预定温度 根据所述流体中的目标物质的浓度来选择，使得可以以最佳的灵敏度进行分析。 用于分析包含在流体中的目标物质的浓度的装置包括容纳石英振荡器的接触室，温度控制器，设置在用于将流体供给到所述接触室中的进料通道中，用于调节流体的温度 期望的预定温度; 以及石英振荡器频率的检测器。

公开(公告)号：US09190523B2

公开(公告)日：2015-11-17

申请号：US13554393

申请日：2012-07-20

申请人： Byung Du Ahn ,  Je Hun Lee ,  Sei-Yong Park ,  Jun Hyun Park ,  Gun Hee Kim ,  Ji Hun Lim ,  Jae Woo Park ,  Jin Seong Park ,  Toshihiro Kugimiya ,  Aya Miki ,  Shinya Morita ,  Tomoya Kishi ,  Hiroaki Tao

发明人： Byung Du Ahn ,  Je Hun Lee ,  Sei-Yong Park ,  Jun Hyun Park ,  Gun Hee Kim ,  Ji Hun Lim ,  Jae Woo Park ,  Jin Seong Park ,  Toshihiro Kugimiya ,  Aya Miki ,  Shinya Morita ,  Tomoya Kishi ,  Hiroaki Tao

IPC分类号： H01L29/786 ,  H01L21/02 ,  H01L29/66 ,  H01L27/12

CPC分类号： H01L29/7869 ,  H01L21/02565 ,  H01L27/1225 ,  H01L29/66969

摘要： An oxide semiconductor includes a first material including at least one selected from the group consisting of zinc (Zn) and tin (Sn), and a second material, where a value acquired by subtracting an electronegativity difference value between the second material and oxygen (O) from the electronegativity difference value between the first material and oxygen (O) is less than about 1.3.

摘要翻译： 氧化物半导体包括包含选自锌（Zn）和锡（Sn）中的至少一种的第一材料和第二材料，其中通过减去第二材料和氧之间的电负性差值获得的值（O ）从第一材料和氧（O）之间的电负性差值小于约1.3。