公开(公告)号：US12070744B2

公开(公告)日：2024-08-27

申请号：US17526943

申请日：2021-11-15

Applicant: ILLUMINA, INC.

Inventor： Ahmadreza Rashedi ,  Gregory Holst ,  Rajagopal Panchapakesan

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/502 ,  G01N35/1002 ,  G01N35/1016 ,  B01L2200/026 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0877 ,  B01L2400/06

Abstract: Valve assemblies and related systems are disclosed. An apparatus includes or comprises a system including or comprising an imaging system, a flow cell interface having a corresponding flow cell receptacle, a stage assembly, and a valve assembly including or comprising a valve and a valve drive assembly. The stage assembly moves the flow cell interface relative to the imaging system and the valve drive assembly is to drive the valve using shaped input signals to reduce vibration imposed on at least one of the stage assembly or another component of the apparatus based on movement of the valve.

公开(公告)号：US20240390887A1

公开(公告)日：2024-11-28

申请号：US18795995

申请日：2024-08-06

Applicant: ILLUMINA, INC.

Inventor： Ahmadreza Rashedi ,  Gregory Holst ,  Rajagopal Panchapakesan

IPC: B01L3/00 ,  G01N35/10

Abstract: Valve assemblies and related systems are disclosed. A method includes imaging a flow cell of a flow cell assembly carried by a flow cell interface using an imaging system of an apparatus and moving the flow cell assembly relative to the imaging system using a stage assembly based on a command from an associated controller. The method also includes driving a motor of the apparatus using shaped input signals to reduce vibration imposed on at least one of the stage assembly or another component of the apparatus based on movement of the motor.

公开(公告)号：US20220339621A1

公开(公告)日：2022-10-27

申请号：US17526943

申请日：2021-11-15

Applicant: ILLUMINA, INC.

Inventor： Ahmadreza Rashedi ,  Gregory Holst ,  Rajagopal Panchapakesan

IPC: B01L3/00 ,  G01N35/10

Abstract: Valve assemblies and related systems are disclosed. An apparatus includes or comprises a system including or comprising an imaging system, a flow cell interface having a corresponding flow cell receptacle, a stage assembly, and a valve assembly including or comprising a valve and a valve drive assembly. The stage assembly moves the flow cell interface relative to the imaging system and the valve drive assembly is to drive the valve using shaped input signals to reduce vibration imposed on at least one of the stage assembly or another component of the apparatus based on movement of the valve.

公开(公告)号：US20240198310A1

公开(公告)日：2024-06-20

申请号：US18540851

申请日：2023-12-14

Applicant: ILLUMINA, INC.

Inventor： Emily Parker ,  Bryan Crane ,  Peter Thomas ,  Megan Blanchard ,  James Osmus ,  Bradley Drews ,  Richard Lemoine ,  Gregory Holst

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00351 ,  B01J2219/005

Abstract: Library preparation systems and methods are disclosed. In an implementation, a modular bay for preparing a library of samples for sequencing, the modular bay comprising a contact dispenser and a working area comprising a working plate receptacle adapted to receive a working plate, a thermocycler, a magnet, and a drawer. The drawer comprising a consumables area adapted to receive a sample plate adapted to contain a sample, and a plurality of consumables for interacting with the sample in the working plate. The contact dispenser is linearly movable in a longitudinal direction between the consumables area and the working area, such that the contact dispenser is configured to (i) move the sample from the sample plate in the consumables area to the working plate in the working area, and (ii) move the plurality of consumables between the consumables area and the working plate in the working area.

公开(公告)号：US20240100518A1

公开(公告)日：2024-03-28

申请号：US18474589

申请日：2023-09-26

Applicant: ILLUMINA, INC.

Inventor： Matthew Hage ,  John Earney ,  Gregory Holst ,  Mark Majette

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0893 ,  B01L2300/0896

Abstract: The presently described techniques relate generally to providing motion feedback (e.g., motion system calibration and/or sample alignment) in the context of an imaging system (such as a time delay and integration (TDI) based imaging system). The architecture and techniques discussed may achieve nanoscale control and calibration of a movement feedback system without a high-resolution encoder subsystem or, in the alternative embodiments, with a lower resolution (and correspondingly less expensive) encoder subsystem than might otherwise be employed. By way of example, certain embodiments described herein relate to ascertaining or calibrating linear motion of a sample holder surface using nanoscale features (e.g., sample sites or nanowells or lithographically patterned features) provided on a surface of the sample holder.

公开(公告)号：US20240033738A1

公开(公告)日：2024-02-01

申请号：US18357006

申请日：2023-07-21

Applicant: ILLUMINA, INC.

Inventor： Michael Burek ,  Gregory Holst ,  Danilo Condello ,  Dustin Blair

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2200/16 ,  B01L2200/12 ,  B01L2300/0893

Abstract: The presently described techniques relate generally to providing motion feedback (e.g., motion system calibration and/or sample alignment) in the context of an imaging system (such as a time delay and integration (TDI) based imaging system). The architecture and techniques discussed may achieve nanoscale control and calibration of a movement feedback system without a high-resolution encoder subsystem or, in the alternative embodiments, with a lower resolution (and correspondingly less expensive) encoder subsystem than might otherwise be employed. By way of example, certain embodiments described herein relate to ascertaining or calibrating linear motion of a sample holder surface using nanoscale features (e.g., sample sites or nanowells or lithographically patterned features) provided on a surface of the sample holder.

公开(公告)号：US20230272837A1

公开(公告)日：2023-08-31

申请号：US18113815

申请日：2023-02-24

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gregory Holst ,  Dustin Blair ,  John Earney ,  Geraint Evans ,  Matthew Hage

IPC: F16F15/03

CPC classification number: F16F15/035 ,  F16F2230/0047

Abstract: An apparatus includes a chassis, a frame, a sample support member, an imaging assembly, an actuation assembly, and a vibration capture assembly. The frame is coupled with the chassis. The sample support member is supported by the frame. The actuation assembly is supported by the frame and is operable to drive movement of the imaging assembly relative to the sample support member. The vibration capture assembly is operable to selectively transition between a plurality of modes, including a damping mode and an isolation mode. In the damping mode, the vibration capture assembly is configured to resist movement of the frame relative to the chassis in response to operation of the actuation assembly. In the isolation mode, the vibration capture assembly is configured to prevent transmission of vibrational movement in the chassis to the frame.

公开(公告)号：US20250004244A1

公开(公告)日：2025-01-02

申请号：US18759291

申请日：2024-06-28

Applicant: ILLUMINA, INC.

Inventor： Dustin Blair ,  Gregory Holst ,  Merek Siu ,  Paul Sangiorgio ,  Dakota Watson ,  Danilo Condello ,  John Earney ,  Peter Newman

IPC: G02B7/40 ,  G02B26/08

Abstract: An imaging system includes a focus tracking module including: a light source to project a first pair of spots on a sample; and a first image sensor to obtain one or more first images of the first pair of spots; a second image sensor to obtain one or more second images of the sample; one or more mirrors optically coupled to the second image sensor, the one or more mirrors positioned in an optical path from the sample to the second image sensor; and a controller to: determine, using at least a first separation distance measurement of the first pair of spots from the one or more first images, a first sample tilt of the sample about a first axis of the imaging system; and actuate, based at least on the first sample tilt, the one or more mirrors to offset the first sample tilt about the first axis.

公开(公告)号：US20240257320A1

公开(公告)日：2024-08-01

申请号：US18405320

申请日：2024-01-05

Applicant: Illumina, Inc.

Inventor： Robert Ezra Langlois ,  Hongji Ren ,  Mohamed Khaled Mohamed Geunady ,  John S. Vieceli ,  Gregory Holst ,  Paul Sangiorgio

IPC: G06T5/80 ,  G06T3/02 ,  G06T7/73

CPC classification number: G06T5/80 ,  G06T3/02 ,  G06T7/74 ,  G06T2207/30072

Abstract: Systems, methods, and apparatuses are described herein. For instance, a detection apparatus may comprise memory and at least one processor. The detection apparatus may be configured to obtain an image comprising at least one feature and a plurality of fiducials. The plurality of fiducials may be arranged in a pattern. The detection apparatus may be configured to determine a plurality of sub-regions of the image. Each sub-region comprises a subset of the fiducials comprised in the image. The detection apparatus may be configured to perform a geometric transform on each sub-region to generate a respective local transform associated with each sub-region. The detection apparatus may be configured to register respective locations of the fiducials comprised in the image based on the respective local transform associated with each sub-region. A size of each sub-region may be selected such that each sub-region is substantially invariant to stage jitter.

公开(公告)号：US20240118203A1

公开(公告)日：2024-04-11

申请号：US18373592

申请日：2023-09-27

Applicant: Illumina, Inc.

Inventor： Shavinesh Sukesh ,  Chia-Hsi Liu ,  Gregory Holst ,  Ahmed Okasha ,  Kurt Danielson ,  John Earney

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N2201/025 ,  G01N2201/068

Abstract: The motion of a mechanical stage may be directed in x-, y-, and/or z-dimensions such that excitation of a resonant frequency f is reduced. In particular, once a resonant frequency f is identified, the acceleration of the stage in the x-, y-, and/or z-dimensions may divided into an even number of acceleration segments or intervals, with the second of each pair of acceleration segments starting 1/(2f) seconds after the start of the initial acceleration segment. The acceleration intervals may be defined by a start time, an amplitude profile, and/or a time duration. In some implementations, the amplitude and time duration of each acceleration pulse may be different. The amplitude and time duration of acceleration steps may be determined and adjusted to compensate for the particular resonance frequency of an individual system, and programmed into a controller for the stage using motor programming controls.