Genetically encoded fluorescent coumarin amino acids
    1.
    发明申请
    Genetically encoded fluorescent coumarin amino acids 失效
    遗传编码荧光香豆素氨基酸

    公开(公告)号:US20080044886A1

    公开(公告)日:2008-02-21

    申请号:US11805247

    申请日:2007-05-21

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将香豆素非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸的蛋白质的方法和相关翻译系统。

    Genetically encoded fluorescent coumarin amino acids
    3.
    发明授权
    Genetically encoded fluorescent coumarin amino acids 失效
    遗传编码荧光香豆素氨基酸

    公开(公告)号:US07807411B2

    公开(公告)日:2010-10-05

    申请号:US11805247

    申请日:2007-05-21

    IPC分类号: C12P21/00

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将香豆素非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸的蛋白质的方法和相关翻译系统。

    Genetically encoded fluorescent coumarin amino acids
    4.
    发明授权
    Genetically encoded fluorescent coumarin amino acids 失效
    遗传编码荧光香豆素氨基酸

    公开(公告)号:US08192952B2

    公开(公告)日:2012-06-05

    申请号:US12227383

    申请日:2007-05-21

    IPC分类号: C12P21/00 C12N15/74 C12N1/21

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将香豆素非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸的蛋白质的方法和相关翻译系统。

    Genetically encoded coumarin amino acids
    5.
    发明申请
    Genetically encoded coumarin amino acids 失效
    遗传编码的香豆素氨基酸

    公开(公告)号:US20090298119A1

    公开(公告)日:2009-12-03

    申请号:US12227383

    申请日:2007-05-21

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将香豆素非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸的蛋白质的方法和相关翻译系统。

    GENETICALLY ENCODED FLUORESCENT COUMARIN AMINO ACIDS
    7.
    发明申请
    GENETICALLY ENCODED FLUORESCENT COUMARIN AMINO ACIDS 审中-公开
    基因编码荧光蛋白氨基酸

    公开(公告)号:US20120329162A1

    公开(公告)日:2012-12-27

    申请号:US13460174

    申请日:2012-04-30

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将香豆素非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸L-(7-羟基香豆素-4-基)乙基甘氨酸的蛋白质的方法和相关翻译系统。

    Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
    9.
    发明申请
    Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine 有权
    含有非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传程序表达

    公开(公告)号:US20070238152A1

    公开(公告)日:2007-10-11

    申请号:US11724039

    申请日:2007-03-13

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将非天然氨基酸苯硒代半胱氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶,编码新合成酶分子的多核苷酸,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸苯基硒半胱氨酸的蛋白质的翻译方法和翻译系统。 本发明还提供了通过蛋白质中的苯基硒代半胱氨酸残基的靶向修饰产生修饰的蛋白质(例如,脂质化蛋白质)的方法。

    Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
    10.
    发明授权
    Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine 有权
    含有非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传程序表达

    公开(公告)号:US08217145B2

    公开(公告)日:2012-07-10

    申请号:US12225261

    申请日:2007-03-13

    摘要: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

    摘要翻译: 本发明涉及tRNA和氨酰-tRNA合成酶的正交对,其可以将非天然氨基酸苯硒代半胱氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶,编码新合成酶分子的多核苷酸,用于鉴定和制备新合成酶的方法,用于产生含有非天然氨基酸苯基硒半胱氨酸的蛋白质的翻译方法和翻译系统。 本发明还提供了通过蛋白质中的苯基硒代半胱氨酸残基的靶向修饰产生修饰的蛋白质(例如,脂质化蛋白质)的方法。