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    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
    2.
    发明授权
    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination 失效
    将重原子含有非天然氨基酸的位点特异性并入蛋白质进行结构测定

    公开(公告)号：US07399619B2

    公开(公告)日：2008-07-15

    申请号：US11137850

    申请日：2005-05-24

    申请人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    发明人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/00 ,  C12N1/21 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

    CPC分类号： C12N9/93 ,  C07H21/04 ,  C12N15/67 ,  C12P21/02

    摘要： Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

    摘要翻译： 提供了翻译系统和包括正交氨酰基tRNA合成酶的其它组合物，其优先用碘化或溴化氨基酸对正交的tRNA进行充电。 还描述了编码这种合成酶的核酸，以及用于产生蛋白质的方法和试剂盒，包括含重原子的氨基酸，例如溴化或碘化​​氨基酸。 还描述了确定蛋白质结构的方法，例如通过使用正交的tRNA /氨基酰基tRNA-合成酶对将重原子特异性并入的蛋白质的蛋白质。

    Adding photoregulated amino acids to the genetic code
    3.
    发明申请
    Adding photoregulated amino acids to the genetic code 审中-公开
    将光调节氨基酸添加到遗传密码中

    公开(公告)号：US20060134746A1

    公开(公告)日：2006-06-22

    申请号：US11233508

    申请日：2005-09-21

    申请人： Alexander Deiters ,  Ning Wu ,  Peter Schultz ,  David King ,  T. Cropp ,  Mohua Bose ,  Dan Groff ,  Jianming Xie ,  Eric Brustad

    发明人： Alexander Deiters ,  Ning Wu ,  Peter Schultz ,  David King ,  T. Cropp ,  Mohua Bose ,  Dan Groff ,  Jianming Xie ,  Eric Brustad

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/22 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/93

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.

    摘要翻译： 生成蛋白质生物合成机理成分的组合物和方法，包括正交亮氨酰-tRNA，正交亮氨酰 - 氨基-tRNA合成酶和亮氨酰-tRNAs /合成酶的正交对，其结合光调节氨基酸，OMe-L-酪氨酸，α-氨基辛酸 酸或O-硝基苄基半胱氨酸进入蛋白质是响应于琥珀选择密码子而提供的。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有光调节氨基酸，OMe-L-酪氨酸，α-氨基辛酸或邻硝基苄基半胱氨酸的蛋白质的方法。