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公开(公告)号：US07579442B2
公开(公告)日：2009-08-25
申请号：US10414796
申请日：2003-04-16
申请人： Joseph J. Bango , John B. Fenn
发明人： Joseph J. Bango , John B. Fenn
摘要： A method and means of providing stromal repair and improved refractive correction by creating corneal stromal collagen tissue with fibril diameter and spacing that duplicates the optical transmission and diffusion characteristics of natural corneal collagen. The repair method includes implanting the collagen scaffold during laser corneal ablation or other interlamellar surgery to improve visual acuity or to preclude the possibility of ectasia.
摘要翻译： 通过产生具有原纤维直径和间隔的角膜基质胶原组织来提供基质修复和改进的屈光矫正的方法和手段，其复制天然角膜胶原的光学透射和扩散特征。 修复方法包括在激光角膜消融或其他层间手术期间植入胶原支架以改善视力或排除扩张的可能性。
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2.发明授权Light transparent multilayer moisture barrier for electrochemical celltester and cell employing same 失效用于电化学细胞器和使用其的细胞的透明多透明屏障
公开(公告)号：US5681666A
公开(公告)日：1997-10-28
申请号：US695092
申请日：1996-08-08
CPC分类号： H01M6/5044 , H01M2/0267 , H01M2/0275 , H01M2/1094 , H01L51/5256 , H01L51/5259
摘要： A light transparent moisture barrier useful for preventing moisture from destroying the effectiveness of a moisture sensitive cell condition tester on an electrochemical cell comprises a plurality of very thin; alternating layers of an inorganic material and an organic material on a flexible, polymeric substrate. The layers are not laminated, but are formed on the substrate by a deposition or coating process and the thickness of any layer is less than 5 microns. The organic material is a hydrophobic polymer and the inorganic material is a metal oxide, nitride, a glass or silicon,
摘要翻译： 用于防止水分破坏电化学电池上的湿敏感细胞状态测试仪的有效性的透明透明屏障包括多个非常薄的; 在柔性聚合物基材上的无机材料和有机材料的交替层。 这些层不是层压的，而是通过沉积或涂覆方法在基底上形成，并且任何层的厚度小于5微米。 有机材料是疏水性聚合物，无机材料是金属氧化物，氮化物，玻璃或硅，
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3.发明申请Method for creating multiply charged ions for MALDI mass spectrometry (ESMALDI) 审中-公开用于MALDI质谱（ESMALDI）产生多重带电离子的方法公开(公告)号：US20080067345A1
公开(公告)日：2008-03-20
申请号：US11786849
申请日：2007-04-14
申请人： John B. Fenn
发明人： John B. Fenn
IPC分类号： B01D59/44
CPC分类号： H01J49/165 , H01J49/0418
摘要： A method of combining ES (ElectroSpray Ionization) and MALDI (Matrix Assisted Laser Desorption Ionization) to create enhanced MALDI mass spectrometry to be referred to as ESMALDI (ElectroSpray Matrix Assisted Laser Desorption Ionization). ESMALDI technology offers substantial advantages over conventional technology. essentially by combining the high ionization efficiency and multiple charging of ions in ESI with the smaller sample requirements and operational simplicity of MALDI. Biologists should thus be able to achieve higher sensitivity, better protein identification, enhanced quantification potential and enhanced structural information. An additional degree of freedom for ESMALDI MS is flexibility in the choice of solvents that can be used in the ESI component of the process. Substantial differences in ESI behavior can occur with the same analyte or mixture of analytes in different solvents. Such differences often provide clues to the properties of the analyte and its ions which cannot be readily obtained from conventional MALDI experiments.
摘要翻译： 将ES（电喷雾离子化）和MALDI（基质辅助激光解吸电离）组合以产生增强的MALDI质谱法被称为ESMALDI（电喷雾矩阵辅助激光解吸电离）的方法。 ESMALDI技术比传统技术具有显着的优势。 基本上通过将ESI中的离子的高电离效率和多次充电与MALDI的较小样品要求和操作简便性相结合。 因此，生物学家应该能够实现更高的灵敏度，更好的蛋白质鉴定，增强的量化潜力和增强的结构信息。 ESMALDI MS的额外自由度在选择可用于该过程的ESI组分的溶剂中具有灵活性。 在不同溶剂中，相同的分析物或分析物混合物可能发生ESI行为的显着差异。 这种差异通常提供了从常规MALDI实验中不能容易地获得的分析物及其离子的性质的线索。
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公开(公告)号：US5581080A
公开(公告)日：1996-12-03
申请号：US309852
申请日：1994-09-21
申请人： John B. Fenn , Chin-Kai Meng , Matthias Mann
发明人： John B. Fenn , Chin-Kai Meng , Matthias Mann
CPC分类号： H01J49/165 , B01D59/44 , H01J49/427
摘要： A production of mass spectra which contain a multiplicity of peaks. The component ions of these peaks, which are multiply charged, are formed by dispersing a solution containing an analyte into a bath gas as highly charged droplets. The analyte is generally a compound of high molecular weight and is of biochemical interest. The methods calculate the molecular weight of the analyte from the measured mass values of the highly charged ions.
摘要翻译： 产生含有多个峰的质谱。 通过将含有分析物的溶液分散到作为高电荷液滴的浴气中形成这些峰的成分离子，其是多重带电的。 分析物通常是高分子量的化合物，具有生物化学意义。 该方法从高电荷离子的测量质量值计算分析物的分子量。
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5.发明授权Method of producing multiply charged ions and for determining molecular weights of molecules by use of the multiply charged ions of molecules 失效产生多电荷离子并通过使用分子的多电荷离子来确定分子的分子量的方法
公开(公告)号：US5130538A
公开(公告)日：1992-07-14
申请号：US773776
申请日：1991-10-10
申请人： John B. Fenn , Matthias Mann , Chin-Kai Meng
发明人： John B. Fenn , Matthias Mann , Chin-Kai Meng
CPC分类号： H01J49/4225 , B01D59/44 , H01J49/04 , H01J49/165 , H01J49/427 , G01N30/7266
摘要： This invention describes the production of mass spectra which contain a multiplicty of peaks. The component ions of these peaks, which are multiply charged, are formed by dispersing a solution containing an analyte into a bath gas as highly charged droplets. The analyte is generally a compound of high molecular weight and is of biochemical interest. The invention also describes methods for calculating the molecular weight of the analyte from the measured mass values of the highly charged ions.
摘要翻译： 本发明描述了包含多重峰的质谱的生产。 通过将含有分析物的溶液分散到作为高电荷液滴的浴气中形成这些峰的成分离子，其是多重带电的。 分析物通常是高分子量的化合物，具有生物化学意义。 本发明还描述了从高电荷离子的测量质量值计算分析物的分子量的方法。
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6.发明授权Process and apparatus for changing the energy of charged particles contained in a gaseous medium 失效用于改变包含在气体介质中的带电粒子的能量的方法和装置
公开(公告)号：US4542293A
公开(公告)日：1985-09-17
申请号：US486642
申请日：1983-04-20
CPC分类号： H01J49/0404 , B01D59/44 , H01J49/165
摘要： A method of changing the energy of charged particles contained in a gas comprises allowing the gas to flow into a region of reduced pressure through a tube like member so that viscous forces exerted on the charged particles by the flowing gas molecules determine the kinetic energy of the charged particles. A potential gradient is maintained along the length of the tube so that the potential energy of the charged particles is changed as they pass through the tube. At the end of the tube a free jet expansion occurs so that the kinetic energy of the charged particles is no longer determined by the flowing gas, so that they can be accelerated to any desired kinetic energy by means of another potential gradient.The invention can be used to interface any high pressure ion source to a magnetic sector mass spectrometer, or to permit the operation of an electrospray ion source with an earthed inlet capillary with either a quadrupole or a magnetic sector mass spectrometer.
摘要翻译： 改变包含在气体中的带电粒子的能量的方法包括允许气体通过管状部件流入减压区域，使得流动的气体分子施加在带电粒子上的粘性力决定了 带电粒子。 沿着管的长度保持电位梯度，使得带电粒子的势能在它们通过管时被改变。 在管的末端发生自由射流膨胀，使得带电粒子的动能不再由流动的气体决定，从而可以通过另一个电位梯度将其加速到任何所需的动能。 本发明可用于将任何高压离子源连接到磁性部门质谱仪，或允许使用具有四极杆或磁性部门质谱仪的接地入口毛细管操作电喷雾离子源。
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7.发明授权Light transparent multilayer moisture barrier for electrochemical cell tester and cell employing same 失效用于电化学电池测试仪和使用其的电池的透明多层防潮屏障
公开(公告)号：US5607789A
公开(公告)日：1997-03-04
申请号：US376274
申请日：1995-01-23
申请人： Jack Treger , Bryan C. Lagos , John B. Fenn , H. Frank Gibbard , Guang Wei
发明人： Jack Treger , Bryan C. Lagos , John B. Fenn , H. Frank Gibbard , Guang Wei
CPC分类号： H01M6/5044 , H01M2/0267 , H01M2/0275 , H01M2/1094 , H01L51/5256 , H01L51/5259
摘要： A light transparent moisture barrier useful for preventing moisture from destroying the effectiveness of a moisture sensitive cell condition tester on an electrochemical cell comprises a plurality of very thin, alternating layers of an inorganic material and an organic material on a flexible, polymeric substrate. The layers are not laminated, but are formed on the substrate by a deposition or coating process and the thickness of any layer is less than 5 microns. The organic material is a hydrophobic polymer and the inorganic material is a metal oxide, nitride, a glass or silicon.
摘要翻译： 用于防止湿气破坏电化学电池上的湿气敏感细胞状态测试仪的有效性的透明透明防潮屏障包括在柔性聚合物基底上的多个非常薄的交替层的无机材料和有机材料。 这些层不是层压的，而是通过沉积或涂覆方法在基底上形成，并且任何层的厚度小于5微米。 有机材料是疏水性聚合物，无机材料是金属氧化物，氮化物，玻璃或硅。
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8.发明授权Method for detection and analysis of inorganic ions in aqueous solutions by electrospray mass spectrometry 失效通过电喷雾质谱法检测和分析水溶液中的无机离子的方法
公开(公告)号：US5523566A
公开(公告)日：1996-06-04
申请号：US278032
申请日：1994-07-20
CPC分类号： H01J49/165
摘要： An electrospray mass spectrometric method for analyzing an aqueous solution containing inorganic ion species first enhances the signals for small inorganic ions in electrospray mass spectrometric analysis of aqueous solutions by substantially diluting a sample of the aqueous solution with an organic solvent. The method also removes solvent molecules and other ligands from the small inorganic ions formed in electrospray ionization before mass analysis is complete, by appropriate application of electric fields to accelerate the ions so they will have energetic collisions with neutral gas molecules.
摘要翻译： 用于分析含有无机离子物质的水溶液的电喷雾质谱法首先通过用有机溶剂基本上稀释水溶液样品来增强水溶液的电喷雾质谱分析中的小无机离子的信号。 该方法还可以通过适当地施加电场来加速离子，使其与中性气体分子发生能量碰撞，从质子分析完成之前的电喷雾电离中形成的小无机离子中除去溶剂分子和其它配体。
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9.发明授权
公开(公告)号：US5306412A
公开(公告)日：1994-04-26
申请号：US46379
申请日：1993-04-08
申请人： Craig M. Whitehouse , John B. Fenn , Shida Shen , Cawthon Smith
发明人： Craig M. Whitehouse , John B. Fenn , Shida Shen , Cawthon Smith
CPC分类号： H01J49/167 , B05B17/0623 , B05B17/063 , G01N30/7246 , G01N30/7266 , H01J49/0454 , G01N2030/6013 , G01N30/7273 , G01N30/7293
摘要： The subject invention teaches the use of mechanical vibration to enhance the electrostatic dispersion of sample solutions into the small, highly charged droplets that can produce ions of solute species for mass spectrometric analysis. Such vibration turns out to be surprisingly effective at ultrasonic frequencies for solutions with flow rates, conductivities and surface tensions too high for stable dispersion by electrostatic forces alone as in conventional electrospray ionization. Several embodiments of the invention are described for purposes of illustration. Other possible embodiments will become apparent to those skilled in the art.
摘要翻译： 本发明教导了使用机械振动来增强样品溶液到可以产生用于质谱分析的溶质物质离子的小的高电荷液滴中的静电分散。 这种振动在超声波频率下对于流速，电导率和表面张力过高，对于通过静电力的稳定分散（如在常规电喷雾离子化）中的解决方案而言，是惊人的有效的。 为了说明的目的描述了本发明的若干实施例。 其他可能的实施例对于本领域技术人员将变得显而易见。
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公开(公告)号：US4531056A
公开(公告)日：1985-07-23
申请号：US486645
申请日：1983-04-20
CPC分类号： H01J49/165 , B01D59/44 , H01J49/044 , H01J49/0477 , G01N30/7273 , G01N30/7293
摘要： An electrospray ion source for a mass spectrometer capable of generating ions from samples dissolved in a solution comprises a capillary tube through which the said solution is pumped into a first chamber maintained substantially at atmospheric pressure and in which an inert gas is flowing in a direction counter to the flow of the solution, and a small orifice in the end wall of the chamber opposite to and aligned with the capillary. A high potential difference is applied between the capillary and the end wall so that the solution is electrosprayed into the chamber and ions characteristic of the sample are formed. These ions are desolvated to a controllable extent by the inert gas, which may also be heated to improve the efficiency of the process and increase the maximum permissible flow rate of solution. The ions so formed pass through the small orifice into a second chamber maintained at a reduced pressure, and into a mass spectrometer. Alternatively an additional pressure reduction stage can be included, so that the ions pass into a third chamber maintained at a still lower pressure in which the mass spectrometer is situated through a conventional nozzle and skimmer arrangement. The ion source is particularly effective for the production of unfragmented and unsolvated ions from thermally unstable or involatile samples, and may be used as a liquid chromatograph--mass spectrometer interface.
摘要翻译： 用于能够从溶解在溶液中的样品产生离子的质谱仪的电喷雾离子源包括毛细管，所述溶液通过该毛细管被泵入保持基本上处于大气压的第一室，并且惰性气体沿反方向流动 到溶液的流动，以及在腔室的端壁中与毛细管相对并与毛细管对准的小孔口。 在毛细管和端壁之间施加高电位差，使得溶液电喷雾到室中并形成样品的特征。 这些离子通过惰性气体被解吸到可控程度，惰性气体也可被加热以提高过程的效率并增加溶液的最大允许流速。 如此形成的离子通过小孔进入保持在减压下的第二室，并进入质谱仪。 或者，可以包括另外的减压阶段，使得离子进入维持在更低压力的第三室中，在该压力下质谱仪通过常规喷嘴和分离器排列设置。 离子源对于从热不稳定或非挥发性样品生产未分散和未溶解的离子特别有效，并且可以用作液相色谱 - 质谱仪界面。
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