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21 条记录 (耗时 0.089 秒)

    Process for amplifying, detecting, and/or cloning nucleic acid sequences
    1.
    发明授权
    Process for amplifying, detecting, and/or cloning nucleic acid sequences 失效
    用于扩增，检测和/或克隆核酸序列的方法

    公开(公告)号：US4800159A

    公开(公告)日：1989-01-24

    申请号：US943948

    申请日：1986-12-17

    申请人： Kary B. Mullis ,  Henry A. Erlich ,  Norman Arnheim ,  Glenn T. Horn ,  Randall K. Saiki ,  Stephen J. Scharf

    发明人： Kary B. Mullis ,  Henry A. Erlich ,  Norman Arnheim ,  Glenn T. Horn ,  Randall K. Saiki ,  Stephen J. Scharf

    IPC分类号： C12Q1/68 ,  C12N15/00 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6858

    摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.

    摘要翻译： 本发明涉及扩增和检测包含在核酸或其混合物中的任何靶核酸序列的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理分离的核酸互补链，延伸引物以形成用作合成所需核酸序列的模板的互补引物延伸产物，以及检测如此扩增的序列。 反应的步骤可以逐步或同时进行，并且可以根据需要经常重复。 此外，通过使用引物扩增其非互补末端含有限制性位点的序列，可以将特异性核酸序列克隆到载体中，并且可以使用扩增过程从现有的较短片段制备核酸片段 。

    Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
    4.
    发明授权
    Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme 失效
    使用热稳定酶扩增，检测和/或克隆核酸序列的方法

    公开(公告)号：US4965188A

    公开(公告)日：1990-10-23

    申请号：US63647

    申请日：1987-06-17

    申请人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

    发明人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

    IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12Q1/6844

    摘要： A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

    摘要翻译： 用于扩增核酸或其混合物中包含的任何靶核酸序列的方法包括用摩尔过量的两个寡核苷酸引物处理所述核酸的分开的互补链，并用热稳定酶扩增引物以形成互补引物延伸产物，其起作用 作为合成所需核酸序列的模板。 可以容易地检测扩增的序列。 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

    Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
    7.
    发明授权
    Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 失效
    用于检测核酸中存在的特异性核苷酸变异和遗传多态性的方法

    公开(公告)号：US5604099A

    公开(公告)日：1997-02-18

    申请号：US457647

    申请日：1995-06-01

    申请人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis

    发明人： Henry A. Erlich ,  Glenn Horn ,  Randall K. Saiki ,  Kary B. Mullis

    IPC分类号： C07K14/74 ,  C12Q1/68 ,  C12N9/12 ,  C12N15/11 ,  C12P19/34

    CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/172

    摘要： Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.

    摘要翻译： 核酸序列中的单个或多个核苷酸变异可通过以下过程在核酸中检测，其中怀疑含有相关核酸的样品用引物，三磷酸核苷酸和用于聚合三磷酸酯的试剂重复处理，然后变性 如果存在，则扩增含有核苷酸变异的序列的过程。 在一个实施方案中，将样品点在膜上并用标记的序列特异性寡核苷酸探针处理。 通过探头上的标签检测探针与样品的杂交。

    Method for detection of polymorphic restriction sites and nucleic acid sequences
    9.
    发明授权
    Method for detection of polymorphic restriction sites and nucleic acid sequences 失效
    检测多态性限制性位点和核酸序列的方法

    公开(公告)号：US4683194A

    公开(公告)日：1987-07-28

    申请号：US716982

    申请日：1985-03-28

    申请人： Randall K. Saiki ,  Henry A. Erlich

    发明人： Randall K. Saiki ,  Henry A. Erlich

    IPC分类号： G01N33/50 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58 ,  C12N15/00 ,  C12P19/34

    CPC分类号： C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q2600/156 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/808 ,  Y10S436/811

    摘要： In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. The nucleic acid is hybridized to the probe and a blocking oligomer may be added, if necessary, to prevent non-specific binding of the probe. Subsequent digestion with a restriction enzyme cleaves those oligomers that have hybridized to the nucleic acid and reformed the restriction site. The resulting cut and uncut labeled oligomers are separated and detected based on the type of probe label.The described method may be used to detect sickle cell anemia.

    摘要翻译： 在用于检测核酸序列中特定限制性位点的存在或不存在的方法中，合成与跨越所述限制性位点的核酸序列的一条链互补的寡核苷酸探针。 探针在更接近限制位点的末端标记。 核酸与探针杂交，如果需要，可以加入阻断低聚物以防止探针的非特异性结合。 随后用限制性内切酶消化已经与核酸杂交的那些寡聚物并重整限制性位点。 基于探针标签的类型分离并检测所得切割和未切割的标记寡聚物。 所述方法可用于检测镰状细胞性贫血。