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公开(公告)号：US6013261A
公开(公告)日：2000-01-11
申请号：US776660
申请日：1997-01-30
IPC分类号： G01N33/53 , A61K39/00 , A61K39/255 , A61P31/12 , C07H21/04 , C07K14/03 , C07K14/125 , C07K16/00 , C12N7/00 , C12N7/01 , C12N15/09 , C12N15/38 , C12N15/869 , C12R1/92 , G01N33/569 , A61K39/12 , A61K39/245
CPC分类号： C12N15/86 , C07K14/005 , A61K2039/53 , C12N2710/16343 , C12N2760/18122 , Y10S424/816
摘要： A recombinant herpes virus is provided which efficiently expresses an exogenous protein of interest in an inoculated animal host to thereby endow the host with excellent immunization. A recombinant herpes virus which incorporates an exogenous gene expression cassette in which a herpes virus-derived gB gene (a gene homologous to herpes simplex virus gB gene) promoter is used as a promoter for expression of an exogenous gene.
摘要翻译： PCT No.PCT / JP96 / 01428 Sec。 371日期1997年1月30日 102（e）1997年1月30日PCT PCT 1996年5月28日PCT公布。 出版物WO96 / 38565 日期1996年12月5日提供一种重组疱疹病毒，其在接种的动物宿主中有效地表达外源性目的蛋白质，从而赋予宿主优异的免疫。 结合外源基因表达盒的重组疱疹病毒，其中使用疱疹病毒来源的gB基因（与单纯疱疹病毒gB基因同源的基因）启动子作为外源基因表达的启动子。
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2.发明授权公开(公告)号：US06544519B1
公开(公告)日：2003-04-08
申请号：US09077098
申请日：1998-05-19
IPC分类号： A61K3938
CPC分类号： C07K14/285 , A61K38/00 , A61K39/00 , Y10S435/851
摘要： A novel peptide obtained from Haemophilus paragallinarum has been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.
摘要翻译： 已经发现从嗜血杆菌获得的新肽可用于预防禽传染性鼻炎。 该多肽诱导血细胞凝集抑制抗体的产生并防止禽传染性鼻炎的感染和发作。 本发明还提供了编码该多肽的基因，用于表达该基因的重组载体，用该载体转化的宿主，在宿主中制备该多肽的方法，用于禽传染性鼻炎的疫苗，其包含该多肽作为活性成分 ，使用该多肽作为免疫原获得的单克隆抗体，以及使用该肽和抗体的诊断剂和禽类感染性鼻炎的治疗剂。
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3.发明授权公开(公告)号：US06919080B2
公开(公告)日：2005-07-19
申请号：US10192584
申请日：2002-07-11
IPC分类号： G01N33/53 , A61K38/00 , A61K39/00 , A61K39/102 , A61K39/395 , A61P31/04 , C07K7/08 , C07K14/195 , C07K14/285 , C12N1/21 , C12N15/09 , C12N15/31 , C12P21/02 , C12P21/08 , C12R1/19 , G01N33/569 , A61K39/12 , A61K39/38 , A61K39/40 , A61K39/42
CPC分类号： C07K14/285 , A61K38/00 , A61K39/00 , Y10S435/851
摘要： A novel peptide obtained from Haemophilus paragallinarum has been found useful for preventing avian infectious coryza. This polypeptide induces production of hemagglutination-inhibition antibody and prevents infection and onset of avian infectious coryza. The invention further provides a gene coding for the polypeptide, a recombinant vector for expression of this gene, a host transformed with this vector, a process for preparing the polypeptide in a host, a vaccine for avian infectious coryza comprising the polypeptide as an active ingredient, a monoclonal antibody obtained using the polypeptide as an immunogen, and a diagnostic agent and a therapeutic agent for avian infectious coryza using the peptide and the antibody.
摘要翻译： 已经发现从嗜血杆菌获得的新肽可用于预防禽传染性鼻炎。 该多肽诱导血细胞凝集抑制抗体的产生并防止禽传染性鼻炎的感染和发作。 本发明还提供了编码该多肽的基因，用于表达该基因的重组载体，用该载体转化的宿主，在宿主中制备该多肽的方法，用于禽传染性鼻炎的疫苗，其包含该多肽作为活性成分 ，使用该多肽作为免疫原获得的单克隆抗体，以及使用该肽和抗体的诊断剂和禽类感染性鼻炎的治疗剂。
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公开(公告)号：US4997767A
公开(公告)日：1991-03-05
申请号：US946329
申请日：1986-12-23
IPC分类号： C12N15/09 , A61K39/29 , C12N15/00 , C12N15/64 , C12N15/70 , C12N15/81 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C12N15/64 , C12N15/70 , Y10S435/911 , Y10S435/94 , Y10S435/942
摘要： Shuttle vectors including a DNA sequence of the yeast Saccharomyces cerevisiae including ars 1.2 micron ori and a marker gene for transformed yeast permitting synthesis of leucine by the transformant; adjacent to said ars 1 a DNA sequence of Escherichia coli which is either in EcoR1--PvuII or EcoR1--TthIII--I fragment of plasmid pBR322; and adjacent to the yeast marker gene the expression control region of the repressible acid phosphatase gene of yeast.
摘要翻译： 穿梭载体包括酵母酿酒酵母的DNA序列，包括1.2微米的穗，以及转化酵母允许转化体合成亮氨酸的标记基因; 与所述ars1相邻的是质粒pBR322的EcoRⅠ-PvuII或EcoR1-TthIII-I片段中的大肠杆菌的DNA序列; 并与酵母标记基因相邻，是酵母抑制性酸性磷酸酶基因的表达控制区。
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5.发明授权Compositions and methods for treatment of Hepatitis C virus-associated diseases 失效治疗丙型肝炎病毒相关疾病的组合物和方法公开(公告)号：US06391542B1
公开(公告)日：2002-05-21
申请号：US08650093
申请日：1996-05-17
申请人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa , Shoji Furasako , Thomas W. Bruice , Walter F. Lima
发明人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa , Shoji Furasako , Thomas W. Bruice , Walter F. Lima
IPC分类号： C12Q168
CPC分类号： C12N15/1131 , C07H21/00 , C07K14/005 , C12N2310/315 , C12N2310/33 , C12N2310/3521 , C12N2310/3527 , C12N2770/24222 , C12Q1/707
摘要： Antisense oligonucleotides are provided which are complementary to and hybridizable with at least a portion of HCV RNA and which are capable of inhibiting the function of the HCV RNA. These oligonucleotides can be administered to inhibit the activity of Hepatitis C virus in vivo or in vitro. These compounds can be used either prophylactically or therapeutically to reduce the severity of diseases associated with Hepatitis C virus, and for diagnosis and detection of HCV and HCV-associated diseases. Methods of using these compounds are also disclosed.
摘要翻译： 提供反义寡核苷酸，其与至少一部分HCV RNA互补并可与其相互杂交，并能够抑制HCV RNA的功能。 可以施用这些寡核苷酸来体内或体外抑制丙型肝炎病毒的活性。 这些化合物可以预防性地或治疗性地用于降低与丙型肝炎病毒相关的疾病的严重性，以及HCV和HCV相关疾病的诊断和检测。 还公开了使用这些化合物的方法。
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公开(公告)号：US06284458B1
公开(公告)日：2001-09-04
申请号：US08397220
申请日：1995-03-09
申请人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa
发明人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa
IPC分类号： C12Q168
CPC分类号： C12N15/1131 , C07H21/00 , C07K14/005 , C12N2310/315 , C12N2310/33 , C12N2310/3521 , C12N2310/3527 , C12N2770/24222 , C12Q1/707
摘要： Antisense oligonucleotides are provided which are complementary to and hybridizable with at least a portion of HCV RNA and which are capable of inhibiting the function of the HCV RNA. These oligonucleotides can be administered to inhibit the activity of Hepatitis C virus in vivo or in vitro. These compounds can be used either prophylactically or therapeutically to reduce the severity of diseases associated with Hepatitis C virus, and for diagnosis and detection of HCV and HCV-associated diseases. Methods of using these compounds are also disclosed.
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公开(公告)号：US4612283A
公开(公告)日：1986-09-16
申请号：US656315
申请日：1984-10-01
IPC分类号： C12N15/09 , A61K39/00 , C07K1/22 , C07K14/005 , C07K14/02 , C07K14/10 , C07K14/18 , C07K14/195 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/865 , A61K39/29
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122
摘要： A method for purification of HBs antigen, which comprises treating an HBs tigen-containing material with an acid, optionally subjecting to salting out with ammonium sulfate, and subjecting the material to a chromatography with hydroxyapatite, by which a highly purified HBs antigen suitable for the preparation of HBV vaccine or diagnostic agent can be obtained in an industrial scale. This method is particularly useful for the purification of HBs antigen-containing material produced by recombinants obtained by DNA recombination technique.
摘要翻译： 一种用于纯化HBs抗原的方法，其包括用酸处理含HBs抗原的材料，任选地用硫酸铵进行盐析，并用羟基磷灰石进行色谱分离，通过该色谱法，适用于 可以以工业规模获得HBV疫苗或诊断剂的制备。 该方法对于通过DNA重组技术获得的重组体产生的含HBs抗原的材料的纯化特别有用。
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8.发明授权Method for purification of HBc antigen and method for measurement of HBc antibody by using said purified HBc antigen 失效用于纯化HBc抗原的方法和使用所述纯化的HBc抗原测量HBc抗体的方法
公开(公告)号：US4839277A
公开(公告)日：1989-06-13
申请号：US811399
申请日：1985-12-20
申请人： Keishin Sugahara , Takayuki Imamura , Fukusaburo Hamada , Nobuya Ohtomo , Haruo Fujita , Kazuhide Yagami
发明人： Keishin Sugahara , Takayuki Imamura , Fukusaburo Hamada , Nobuya Ohtomo , Haruo Fujita , Kazuhide Yagami
IPC分类号： C07K14/02 , G01N33/576
CPC分类号： C07K14/005 , G01N33/5762 , C12N2730/10122 , Y10S436/82
摘要： A method for the purification of HBc antigen, particularly HBc antigen produced by recombinant organisms by means of DNA recombination technique, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid and then resulting precipitates of lipid and contaminant proteins are removed, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger, and a method for measuring HBc antibody by using said purified HBc antigen in a passive hemagglutination method, EIA method or RIA method.
摘要翻译： 一种通过DNA重组技术纯化HBc抗原，特别是由重组生物产生的HBc抗原的方法，其包括使含有HBc抗原的溶液进行酸处理，其中将含有HBc抗原的溶液酸化至pH范围 通过添加酸高于6，然后除去所得到的脂质和污染蛋白质沉淀物，然后将含有HBc抗原的酸处理溶液用阴离子交换剂进行离子交换色谱，以及通过使用所述方法测量HBc抗体 被动血凝法纯化HBc抗原，EIA法或RIA法。
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公开(公告)号：US4738926A
公开(公告)日：1988-04-19
申请号：US709711
申请日：1985-03-08
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122 , Y10S435/803 , Y10S435/82 , Y10S530/826
摘要： Industrially useful method for the purification of HBs antigen produced by a recombinant organism being capable of producing HBs antigen which is prepared by means of DNA recombination technique, which comprises subjecting an HBs antigen-containing material produced by a recombinant organism to an adsorption chromatography with a silica, optionally followed by a gel filtration and further an adsorption chromatography with a hydroxyapatite, and then eluting the HBs antigen, preferably, with a buffer having a pH 9 or more which is incorporated with urea. The purification method can give a highly pure HBs antigen suitable for the preparation of hepatitis B vaccine in a large scale from recombinant organisms prepared by DNA recombination technique.
摘要翻译： 能够产生通过DNA重组技术制备的HBs抗原产生的HBs抗原产生的工业上有用的方法，该方法包括使由重组生物产生的含HBs抗原的材料进行吸附色谱， 二氧化硅，任选地随后进行凝胶过滤，并进一步用羟基磷灰石进行吸附色谱，然后优选用与尿素结合的pH9或更高的缓冲液洗脱HBs抗原。 纯化方法可以通过DNA重组技术制备重组生物体，从而大规模制备适合于乙型肝炎疫苗制备的高纯度HBs抗原。
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公开(公告)号：US06423489B1
公开(公告)日：2002-07-23
申请号：US08452841
申请日：1995-05-30
申请人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa , Shoji Furusako , Thomas W. Bruice , Walter F. Lima
发明人： Kevin P. Anderson , Ronnie C. Hanecak , Kazuya Hoshiko , Chikateru Nozaki , Tsukasa Nishihara , Hiroshi Nakatake , Fukusaburo Hamada , Tatsuo Eto , Shinichi Furukawa , Shoji Furusako , Thomas W. Bruice , Walter F. Lima
IPC分类号： C12Q168
CPC分类号： C12N15/1131 , C07H21/00 , C07K14/005 , C12N2310/315 , C12N2310/33 , C12N2310/3521 , C12N2310/3527 , C12N2770/24222 , C12Q1/707
摘要： Antisense oligonucleotides are provided which are complementary to and hybridizable with at least a portion of HCV RNA and which are capable of inhibiting the function of the HCV RNA. These oligonucleotides can be administered to inhibit the activity of Hepatitis C virus in vivo or in vitro. These compounds can be used either prophylactically or therapeutically to reduce the severity of diseases associated with Hepatitis C virus, and for diagnosis and detection of HCV and HCV-associated diseases. Methods of using these compounds are also disclosed.
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