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15 条记录 (耗时 0.049 秒)

    Determination of oligonucleotides for therapeutics, diagnostics and research reagents
    4.
    发明授权
    Determination of oligonucleotides for therapeutics, diagnostics and research reagents 失效
    测定治疗，诊断和研究试剂的寡核苷酸

    公开(公告)号：US5686242A

    公开(公告)日：1997-11-11

    申请号：US330000

    申请日：1994-10-27

    申请人： Thomas W. Bruice ,  Walter F. Lima

    发明人： Thomas W. Bruice ,  Walter F. Lima

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  G01N33/53

    CPC分类号： A61K31/70 ,  C12Q1/6811

    摘要： Oligonucleotides which selectively bind to target biomolecules are determined by in vitro assay of a pool of random oligonucleotides for activity against said biomolecules, followed by recovery and characterization of selected oligonucleotides. Oligonucleotides so determined may be utilized for therapeutic, diagnostic and research reagent purposes.

    摘要翻译： 选择性地结合靶生物分子的寡核苷酸通过体外测定随机寡核苷酸库来测定针对所述生物分子的活性，随后恢复和表征所选择的寡核苷酸。 如此确定的寡核苷酸可用于治疗，诊断和研究试剂目的。

    Determination of oligonucleotides for therapeutics, diagnostics and research reagents
    5.
    发明授权
    Determination of oligonucleotides for therapeutics, diagnostics and research reagents 失效
    测定治疗，诊断和研究试剂的寡核苷酸

    公开(公告)号：US6022691A

    公开(公告)日：2000-02-08

    申请号：US965908

    申请日：1997-11-07

    申请人： Thomas W. Bruice ,  Walter F. Lima

    发明人： Thomas W. Bruice ,  Walter F. Lima

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  G01N33/53

    CPC分类号： A61K31/70 ,  C12Q1/6811

    摘要： Oligonucleotides which selectively bind to target biomolecules are determined by in vitro assay of a pool of random oligonucleotides for activity against the biomolecules, followed by recovery and characterization of selected oligonucleotides. Oligonucleotides so determined may be utilized for therapeutic, diagnostic and research reagent purposes.

    摘要翻译： 选择性结合靶生物分子的寡核苷酸通过体外测定随机寡核苷酸库来测定生物分子的活性，随后选择寡核苷酸的恢复和表征。 如此确定的寡核苷酸可用于治疗，诊断和研究试剂目的。

    Modulation of gene expression through interference with RNA secondary structure
    8.
    发明授权
    Modulation of gene expression through interference with RNA secondary structure 失效
    通过干扰RNA二级结构调节基因表达

    公开(公告)号：US5866698A

    公开(公告)日：1999-02-02

    申请号：US227180

    申请日：1994-04-13

    申请人： David Ecker ,  Timothy A. Vickers ,  Thomas W. Bruice

    发明人： David Ecker ,  Timothy A. Vickers ,  Thomas W. Bruice

    IPC分类号： C12N15/113 ,  C12N15/63 ,  C12N15/67 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1132 ,  C12N15/63 ,  C12N15/67 ,  C12N2310/15 ,  C12N2310/315

    摘要： Methods for modulating the expression of viral genes are provided by selecting a portion of RNA coded by the gene, said RNA portion having subportions forming a secondary structure, and contacting the RNA with oligonucleotide of 6 to 50 which can bind with at least one of said subportions of the RNA. In accordance with the preferred embodiments, oligonucleotides are designed to bind to RNA secondary structures which are of significance to the expression of the gene coding for said RNA. In accordance with a preferred embodiment, methods of treatment of human immunodeficiency virus are similarly disclosed wherein the oligonucleotides are targeted at the CAR or gag-pol region of HIV RNA.

    摘要翻译： 通过选择由基因编码的RNA的一部分来提供调节病毒基因表达的方法，所述RNA部分具有形成二级结构的子部分，并使RNA与6至50的寡核苷酸接触，所述寡核苷酸可与所述 RNA的部分。 根据优选的实施方案，设计寡核苷酸以结合RNA二级结构，这对编码所述RNA的基因的表达具有重要意义。 根据优选实施方案，类似地公开了治疗人类免疫缺陷病毒的方法，其中所述寡核苷酸靶向HIV RNA的CAR或gag-pol区域。