公开(公告)号：US20070275435A1

公开(公告)日：2007-11-29

申请号：US11625847

申请日：2007-01-23

申请人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

发明人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

IPC分类号： C12Q1/02 ,  C12M1/00

CPC分类号： C12M41/36 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34

摘要： Disclosed herein are a cell culture chip for monitoring a cell culture in real time and a method of monitoring the cell culture using the cell culture chip. The cell culture chip includes a cell culture chamber formed by side walls of a non-conductive material and a bottom layer of an insulating material and capable of accommodating a cell culture media. The cell culture chip also includes a semiconductor layer disposed under the bottom layer, a metal layer disposed under the semiconductor layer, and an electrode disposed in the cell culture chamber. The cell culture chip monitors both the states of cells attached to walls of the cell culture chamber and the states of cells floating in the cell culture media.

摘要翻译： 本文公开了用于实时监测细胞培养物的细胞培养芯片和使用细胞培养芯片监测细胞培养的方法。 细胞培养芯片包括由非导电材料的侧壁和绝缘材料的底层形成并能够容纳细胞培养基的细胞培养室。 细胞培养芯片还包括设置在底层下方的半导体层，设置在半导体层下方的金属层和设置在细胞培养室中的电极。 细胞培养芯片监测附着于细胞培养室壁的细胞状态和悬浮在细胞培养基中的细胞状态。

公开(公告)号：US20080072994A1

公开(公告)日：2008-03-27

申请号：US11846794

申请日：2007-08-29

申请人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

发明人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

IPC分类号： B65B1/14

CPC分类号： B01L9/527 ,  B01L3/5021 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12M23/16

摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.

摘要翻译： 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。

公开(公告)号：US20100329931A1

公开(公告)日：2010-12-30

申请号：US12833095

申请日：2010-07-09

申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC分类号： G01N27/00

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20090170211A1

公开(公告)日：2009-07-02

申请号：US12272183

申请日：2008-11-17

申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC分类号： G01N27/00

CPC分类号： G01N33/54373

摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20110183325A1

公开(公告)日：2011-07-28

申请号：US13079926

申请日：2011-04-05

申请人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

发明人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

IPC分类号： C12Q1/68 ,  C12N13/00

CPC分类号： C12Q1/68 ,  C12M47/06 ,  C12N15/1006

摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

摘要翻译： 本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。 该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

公开(公告)号：US20110177588A1

公开(公告)日：2011-07-21

申请号：US13079910

申请日：2011-04-05

申请人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

发明人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

IPC分类号： C12M1/42 ,  C12M1/00

CPC分类号： C12Q1/68 ,  C12M47/06 ,  C12N15/1006

摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

公开(公告)号：US20100204065A1

公开(公告)日：2010-08-12

申请号：US12762596

申请日：2010-04-19

申请人： Su-hyeon KIM ,  In-ho LEE ,  Jun-hong MIN

发明人： Su-hyeon KIM ,  In-ho LEE ,  Jun-hong MIN

IPC分类号： C40B50/14

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00432 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00673 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743

摘要： A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.

摘要翻译： 提供了包含水凝胶和固定在水凝胶的离散区域中的多个探针的微阵列及其制备方法。 当使用微阵列和方法时，不需要固体底物，并且许多生物分子可以以小体积固定，从而获得高灵敏度。 由于可以切割凝胶以获得许多片，因此可以一次制备许多微阵列。

公开(公告)号：US20070238109A1

公开(公告)日：2007-10-11

申请号：US11553657

申请日：2006-10-27

申请人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

发明人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12N15/1006 ,  C12N15/1003

摘要： Provided is a method of purifying nucleic acid, the method including: contacting a nucleic acid-containing sample and a solution containing a kosmotropic salt on a solid support having a hydrophilic functional group on its surface to bind the nucleic acid to the solid support. Since the solid support is used as it is without any surface treatment, manufacture of the apparatus is very easy, and nucleic acid can be bound to the solid support without specific additives in a wide pH range, so that the apparatus can be used for a Lab-On-a-Chip.

摘要翻译： 提供了一种纯化核酸的方法，所述方法包括：使含核酸的样品和含有可变色盐的溶液在其表面上具有亲水性官能团的固体支持物上接触，以将核酸结合到固体支持物上。 由于固体支持物原样使用而没有进行任何表面处理，所以装置的制造非常容易，并且核酸可以在宽的pH范围内结合固体支持物而没有特定的添加剂，使得该装置可以用于 实验室芯片

公开(公告)号：US20100181209A1

公开(公告)日：2010-07-22

申请号：US12637245

申请日：2009-12-14

申请人： Kyu-tae YOO ,  Sung-ouk JUNG ,  Jun-hong MIN ,  Ji-na NAMGOONG ,  Soo-hyung CHOI ,  Jeo-young SHIM

发明人： Kyu-tae YOO ,  Sung-ouk JUNG ,  Jun-hong MIN ,  Ji-na NAMGOONG ,  Soo-hyung CHOI ,  Jeo-young SHIM

IPC分类号： G01N27/26

CPC分类号： G01N33/54306

摘要： Provided is a method of sensing biomolecules using a bioFET, the method including: forming a layer including Au on a gate of the bioFET; forming a probe immobilized on a substrate separated from the gate by a predetermined distance, and a biomolecule having a thiol group (—SH) which is incompletely bonded to the probe; reacting the probe with a sample including a target molecule; and measuring a current flowing in a channel region between a source and a drain of the bioFET.

摘要翻译： 提供了一种使用bioFET感测生物分子的方法，该方法包括：在bioFET的栅极上形成包含Au的层; 形成固定在与栅极分离预定距离的基板上的探针，以及具有与探针不完全结合的硫醇基（-SH）的生物分子; 使探针与包含靶分子的样品反应; 以及测量在bioFET的源极和漏极之间的沟道区域中流动的电流。

公开(公告)号：US20070269819A1

公开(公告)日：2007-11-22

申请号：US11620961

申请日：2007-01-08

申请人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

发明人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可变性盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。