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    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING
    4.
    发明申请
    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING 审中-公开
    使用氢键连接固体支持的亲水表面上的核酸的方法和装置

    公开(公告)号：US20070238109A1

    公开(公告)日：2007-10-11

    申请号：US11553657

    申请日：2006-10-27

    申请人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    发明人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12N15/1006 ,  C12N15/1003

    摘要： Provided is a method of purifying nucleic acid, the method including: contacting a nucleic acid-containing sample and a solution containing a kosmotropic salt on a solid support having a hydrophilic functional group on its surface to bind the nucleic acid to the solid support. Since the solid support is used as it is without any surface treatment, manufacture of the apparatus is very easy, and nucleic acid can be bound to the solid support without specific additives in a wide pH range, so that the apparatus can be used for a Lab-On-a-Chip.

    摘要翻译： 提供了一种纯化核酸的方法，所述方法包括：使含核酸的样品和含有可变色盐的溶液在其表面上具有亲水性官能团的固体支持物上接触，以将核酸结合到固体支持物上。 由于固体支持物原样使用而没有进行任何表面处理，所以装置的制造非常容易，并且核酸可以在宽的pH范围内结合固体支持物而没有特定的添加剂，使得该装置可以用于 实验室芯片

    METHOD AND APPARATUS FOR CONCENTRATING AND AMPLIFYING NUCLEIC ACID IN SINGLE MICRO CHAMBER
    5.
    发明申请
    METHOD AND APPARATUS FOR CONCENTRATING AND AMPLIFYING NUCLEIC ACID IN SINGLE MICRO CHAMBER 有权
    在单个微室中浓缩和放大核酸的方法和装置

    公开(公告)号：US20070269819A1

    公开(公告)日：2007-11-22

    申请号：US11620961

    申请日：2007-01-08

    申请人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

    发明人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

    摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

    摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可变性盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

    CELL CULTURE CHIP AND METHOD FOR REAL-TIME MONITORING OF A CELL CULTURE USING THE SAME
    6.
    发明申请
    CELL CULTURE CHIP AND METHOD FOR REAL-TIME MONITORING OF A CELL CULTURE USING THE SAME 审中-公开
    用于实时监测细胞培养物的细胞培养物芯片和方法

    公开(公告)号：US20070275435A1

    公开(公告)日：2007-11-29

    申请号：US11625847

    申请日：2007-01-23

    申请人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

    发明人： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

    IPC分类号： C12Q1/02 ,  C12M1/00

    CPC分类号： C12M41/36 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34

    摘要： Disclosed herein are a cell culture chip for monitoring a cell culture in real time and a method of monitoring the cell culture using the cell culture chip. The cell culture chip includes a cell culture chamber formed by side walls of a non-conductive material and a bottom layer of an insulating material and capable of accommodating a cell culture media. The cell culture chip also includes a semiconductor layer disposed under the bottom layer, a metal layer disposed under the semiconductor layer, and an electrode disposed in the cell culture chamber. The cell culture chip monitors both the states of cells attached to walls of the cell culture chamber and the states of cells floating in the cell culture media.

    摘要翻译： 本文公开了用于实时监测细胞培养物的细胞培养芯片和使用细胞培养芯片监测细胞培养的方法。 细胞培养芯片包括由非导电材料的侧壁和绝缘材料的底层形成并能够容纳细胞培养基的细胞培养室。 细胞培养芯片还包括设置在底层下方的半导体层，设置在半导体层下方的金属层和设置在细胞培养室中的电极。 细胞培养芯片监测附着于细胞培养室壁的细胞状态和悬浮在细胞培养基中的细胞状态。

    SENSING SWITCH AND DETECTING METHOD USING THE SAME
    7.
    发明申请
    SENSING SWITCH AND DETECTING METHOD USING THE SAME 有权
    感应开关及其检测方法

    公开(公告)号：US20100329931A1

    公开(公告)日：2010-12-30

    申请号：US12833095

    申请日：2010-07-09

    申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

    发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

    IPC分类号： G01N27/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    SENSING SWITCH AND DETECTING METHOD USING THE SAME
    8.
    发明申请
    SENSING SWITCH AND DETECTING METHOD USING THE SAME 有权
    感应开关及其检测方法

    公开(公告)号：US20090170211A1

    公开(公告)日：2009-07-02

    申请号：US12272183

    申请日：2008-11-17

    申请人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

    发明人： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

    IPC分类号： G01N27/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    METHOD OF ELECTRICALLY DETECTING BIOMOLECULE
    9.
    发明申请
    METHOD OF ELECTRICALLY DETECTING BIOMOLECULE 审中-公开
    电生物分析方法

    公开(公告)号：US20100181209A1

    公开(公告)日：2010-07-22

    申请号：US12637245

    申请日：2009-12-14

    申请人： Kyu-tae YOO ,  Sung-ouk JUNG ,  Jun-hong MIN ,  Ji-na NAMGOONG ,  Soo-hyung CHOI ,  Jeo-young SHIM

    发明人： Kyu-tae YOO ,  Sung-ouk JUNG ,  Jun-hong MIN ,  Ji-na NAMGOONG ,  Soo-hyung CHOI ,  Jeo-young SHIM

    IPC分类号： G01N27/26

    CPC分类号： G01N33/54306

    摘要： Provided is a method of sensing biomolecules using a bioFET, the method including: forming a layer including Au on a gate of the bioFET; forming a probe immobilized on a substrate separated from the gate by a predetermined distance, and a biomolecule having a thiol group (—SH) which is incompletely bonded to the probe; reacting the probe with a sample including a target molecule; and measuring a current flowing in a channel region between a source and a drain of the bioFET.

    摘要翻译： 提供了一种使用bioFET感测生物分子的方法，该方法包括：在bioFET的栅极上形成包含Au的层; 形成固定在与栅极分离预定距离的基板上的探针，以及具有与探针不完全结合的硫醇基（-SH）的生物分子; 使探针与包含靶分子的样品反应; 以及测量在bioFET的源极和漏极之间的沟道区域中流动的电流。

    APPARATUS FOR INTRODUCING FLUID INTO MICROFLUIDIC CHIP BY USING CENTRIFUGAL FORCE, A SYSTEM INCLUDING THE APPARATUS, AND A METHOD OF USING THE APPARATUS
    10.
    发明申请
    APPARATUS FOR INTRODUCING FLUID INTO MICROFLUIDIC CHIP BY USING CENTRIFUGAL FORCE, A SYSTEM INCLUDING THE APPARATUS, AND A METHOD OF USING THE APPARATUS 有权
    通过使用离心力将流体引入微流化芯片的装置，包括装置的系统以及使用装置的方法

    公开(公告)号：US20080072994A1

    公开(公告)日：2008-03-27

    申请号：US11846794

    申请日：2007-08-29

    申请人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

    发明人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

    IPC分类号： B65B1/14

    CPC分类号： B01L9/527 ,  B01L3/5021 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12M23/16

    摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.

    摘要翻译： 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。