公开(公告)号：US12070731B2

公开(公告)日：2024-08-27

申请号：US17505075

申请日：2021-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin Brenan ,  Jamie Cho ,  Javier Garcia ,  Robert Hess ,  Tanya Kanigan ,  Arrin Katz ,  Namyong Kim ,  John Linton ,  Shailesh Srivastava ,  Karl Yoder

IPC: B01L3/00 ,  B01J19/00 ,  B01L3/02

CPC classification number: B01J19/0046 ,  B01L3/50857 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/5025 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16

Abstract: A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US20180361345A1

公开(公告)日：2018-12-20

申请号：US15978523

申请日：2018-05-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert Hess ,  John Linton ,  Tanya S. Kanigan ,  Colin Brenan ,  Can Ozbal

IPC: B01J19/00 ,  G01N30/60 ,  C12M1/32 ,  B01L3/00

Abstract: The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

公开(公告)号：US09968903B2

公开(公告)日：2018-05-15

申请号：US15131101

申请日：2016-04-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert Hess ,  John Linton ,  Tanya S. Kanigan ,  Colin Brenan ,  Can Ozbal

IPC: B01J19/00 ,  B01L3/02 ,  B01L3/00 ,  C40B60/14 ,  G01N30/46 ,  G01N30/60 ,  B01L99/00 ,  C12M1/32 ,  G01N30/82 ,  G01N30/84

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L99/00 ,  B01L2200/0657 ,  B01L2300/0845 ,  C12M23/12 ,  C40B60/14 ,  G01N30/466 ,  G01N30/6091 ,  G01N30/6095 ,  G01N30/82 ,  G01N2030/8417

Abstract: The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

公开(公告)号：US11154834B2

公开(公告)日：2021-10-26

申请号：US16284953

申请日：2019-02-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin Brenan ,  Jamie Cho ,  Javier Garcia ,  Robert Hess ,  Tanya Kanigan ,  Arrin Katz ,  Namyong Kim ,  John Linton ,  Shailesh Srivastava ,  Karl Yoder

IPC: B01J19/00 ,  B01L3/00 ,  B01L3/02

Abstract: A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nanovolume channel having at least one interior surface in communication with the at least one exterior surface. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US09428800B2

公开(公告)日：2016-08-30

申请号：US14195358

申请日：2014-03-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John Linton ,  Arrin Katz ,  Colin Brenan ,  Karl Yoder ,  Robert Hess ,  Leila Madrone ,  Robert Ellis ,  Tanya Kanigan ,  Kristine Friesen ,  Thomas Morrison ,  Javier Garcia

IPC: G01N31/22 ,  G01N21/01 ,  G01N21/77 ,  C12Q1/68 ,  B01L3/00 ,  G01N21/03 ,  G01N21/15 ,  G01N21/25 ,  B01L7/00 ,  G01N21/05

CPC classification number: C12Q1/6858 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/508 ,  B01L3/50853 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/16 ,  B01L2300/1822 ,  B01L2300/1838 ,  G01N21/03 ,  G01N21/15 ,  G01N21/253 ,  G01N2021/0346 ,  G01N2021/056 ,  G01N2021/158 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

Abstract translation: 用于容纳至少一个样品和试剂用于分析的系统。 该系统包括一对平行的盖，其至少一个是透光的，其中一对透光盖形成顶部，并且其中一对形成底部。 框架设置在盖之间，以相对于盖限定内部空间。 框架和盖子彼此相关联以形成壳体，该壳体基本上与液体紧密。 微流体阵列设置在内部体积中。 阵列包括具有一对相对表面的厚度的材料片，以及穿过表面之间的厚度的多个通孔，通孔包含至少一个样品和试剂。

公开(公告)号：US20140179566A1

公开(公告)日：2014-06-26

申请号：US14195358

申请日：2014-03-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LINTON ,  Arrin Katz ,  Colin Brenan ,  Karl Yoder ,  Robert Hess ,  Leila Hasan ,  Robert Ellis ,  Tanya Kanigan ,  Kristine Friesen ,  Thomas Morrison ,  Javier Garcia

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/508 ,  B01L3/50853 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/16 ,  B01L2300/1822 ,  B01L2300/1838 ,  G01N21/03 ,  G01N21/15 ,  G01N21/253 ,  G01N2021/0346 ,  G01N2021/056 ,  G01N2021/158 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

Abstract translation: 用于容纳至少一个样品和试剂用于分析的系统。 该系统包括一对平行的盖，其至少一个是透光的，其中一对透光盖形成顶部，并且其中一对形成底部。 框架设置在盖之间，以相对于盖限定内部空间。 框架和盖子彼此相关联以形成壳体，该壳体基本上与液体紧密。 微流体阵列设置在内部体积中。 阵列包括具有一对相对表面的厚度的材料片，以及穿过表面之间的厚度的多个通孔，通孔包含至少一个样品和试剂。