Enzyme with endo-1,3(4)-.beta.-glucanase activity
    1.
    发明授权
    Enzyme with endo-1,3(4)-.beta.-glucanase activity 失效
    具有内切-1,3(4)-β-葡聚糖酶活性的酶

    公开(公告)号:US6140096A

    公开(公告)日:2000-10-31

    申请号:US98580

    申请日:1998-06-17

    摘要: An enzyme having endo-1,3(4)-.beta.-glucanase activity is described which is encoded by the DNA sequence ATGTGGTCTCCCAAGGTTGCTGCTGCCGTCCTCGCCTTTGTTGGTGCTACCAACGCCT GGCAGCCCCCGACCTACAGCGGCTTCAACTTGGTCTGGACTGACACCTTCGCTGGCAACGGTGGCACTTCTCCTA ACCAGAACAACTGGAACATCATCACCGGAAACTTGAACGTCAACGCCGAGCAGGAGACCTACTCCTCCAGCACC GCCAATGTTCAGCTCAGTGGTGGCAGCACCCTTCAGCTGGTCCCCTGGAGAGACAGCAGCAAGGGAACCAGCAC CTTTGGTGGCTGGACCTCCGGTCGTCTTGAGTCCAAGTACACATTCACTCCCGCGGCCGGCAAGGTCACCCGTCTT GAAGCCGCCATCCGCTTCGGCAGCAACGCTCAGGCCAACAAGCAGGGTATCTGGCCTGCTTTCTGGATGCTGGGT GACTCCCTCCGTCAACCGGGCGGCAGCTGGCCCAACTGTGGTGAGATCGACATCATGGAGACTGTCGACGGCCA GGCTACCGGCCACGGTACCCTTCACTGCGACGTCTACCCCGGCGGTATCTGCAACGAGGGTAACGGTATTGGAGG CCCTGTCAACATCGCCAACGTCAACGACTGGCACGCTTGGCGTGTTGAGATCGACCGCACTCCCAGCAGCTGGCA ATCCGAGACCCTCACCTGGTCCCTCGACGGCACCATCTACTTCCAGATCACTGGCTCTCGCATTGGCAACCAGGG CGTCTGGAACAACATTGCTCACAGCCCCCTCTTCTTCATTCTTAACGTTGCTGTCGGTGGCAACTGGCCTGGCAAC CCCAACAGCGCTACCCTCGATGGCTACGGAAGCATGATGGAGGTTGGCTACGTCGCTCAGTACTCTACCTAA (SEQ ID NO:3).

    摘要翻译: 酶具有内切-1,3(4) - β葡聚糖酶活性被描述这是由DNA序列ATGTGGTCTCCCAAGGTTGCTGCTGCCGTCCTCGCCTTTGTTGGTGCTACCAACGCCT GGCAGCCCCCGACCTACAGCGGCTTCAACTTGGTCTGGACTGACACCTTCGCTGGCAACGGTGGCACTTCTCCTA ACCAGAACAACTGGAACATCATCACCGGAAACTTGAACGTCAACGCCGAGCAGGAGACCTACTCCTCCAGCACC GCCAATGTTCAGCTCAGTGGTGGCAGCACCCTTCAGCTGGTCCCCTGGAGAGACAGCAGCAAGGGAACCAGCAC CTTTGGTGGCTGGACCTCCGGTCGTCTTGAGTCCAAGTACACATTCACTCCCGCGGCCGGCAAGGTCACCCGTCTT GAAGCCGCCATCCGCTTCGGCAGCAACGCTCAGGCCAACAAGCAGGGTATCTGGCCTGCTTTCTGGATGCTGGGT GACTCCCTCCGTCAACCGGGCGGCAGCTGGCCCAACTGTGGTGAGATCGACATCATGGAGACTGTCGACGGCCA GGCTACCGGCCACGGTACCCTTCACTGCGACGTCTACCCCGGCGGTATCTGCAACGAGGGTAACGGTATTGGAGG CCCTGTCAACATCGCCAACGTCAACGACTGGCACGCTTGGCGTGTTGAGATCGACCGCACTCCCAGCAGCTGGCA ATCCGAGACCCTCACCTGGTCCCTCGACGGCACCATCTACTTCCAGATCACTGGCTCTCGCATTGGCAACCAGGG CGTCTGGAACAACATTGCTCACAGCCCCCTCTTCTTCATTCTTAACGTTGCTGTCGGTGGCAACTGGCCTGGCAAC CCCAACAGCGCTACCCTCGATGGCTACGGAAGCATGATGGAGGTTGGCTACGTCGCTCAGTACTCTACCTAA(SEQ编码 编号:3)。

    Enzyme with .beta.-(1-6)- endoglucanase activity
    3.
    发明授权
    Enzyme with .beta.-(1-6)- endoglucanase activity 失效
    具有β-(1-6) - 内切葡聚糖酶活性的酶

    公开(公告)号:US6022723A

    公开(公告)日:2000-02-08

    申请号:US40699

    申请日:1998-03-18

    摘要: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译: 本发明涉及由DNA序列编码的β-(1-6) - 内葡聚糖酶活性的酶,该DNA序列a)包含SEQ ID No.3所示的DNA序列,或b)包含DNA的类似物 序列号3所示的序列,其i)与SEQ ID No.3所示的DNA序列同源,和/或ii)与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交, 和/或iii)编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-(1-6) - 葡聚糖酶,如SEQ ID No.4所示,源自哈茨木霉,CBS 243.71。 本发明还涉及编码该酶的DNA构建体,该酶的制备方法,含该酶的酶制剂,以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。

    Enzyme with .beta.-(1-6)-endoglucanase activity
    4.
    发明授权
    Enzyme with .beta.-(1-6)-endoglucanase activity 失效
    具有β-(1-6) - 葡聚糖酶活性的酶

    公开(公告)号:US5770406A

    公开(公告)日:1998-06-23

    申请号:US745977

    申请日:1996-11-08

    摘要: The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

    摘要翻译: 本发明涉及由DNA序列编码的β-(1-6) - 内葡聚糖酶活性的酶,该DNA序列a)包含SEQ ID No.3所示的DNA序列,或b)包含DNA的类似物 序列号3所示的序列,其i)与SEQ ID No.3所示的DNA序列同源,和/或ii)与与SEQ ID No.3所示的DNA序列相同的寡核苷酸探针杂交, 和/或iii)编码与由包含SEQ ID No.3所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对纯化的抗体产生的抗体具有免疫反应性的多肽 β-(1-6) - 葡聚糖酶,如SEQ ID No.4所示,源自哈茨木霉,CBS 243.71。 本发明还涉及编码该酶的DNA构建体,该酶的制备方法,含该酶的酶制剂,以及该酶和酶制剂用于含β-葡聚糖的材料的降解或修饰的用途。