公开(公告)号：US10351907B2

公开(公告)日：2019-07-16

申请号：US14515741

申请日：2014-10-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/6869 ,  B01J19/00 ,  B01F13/00 ,  B01F13/02 ,  B01L3/00 ,  G01N35/00 ,  G01N35/10 ,  C12Q1/6874 ,  G02B21/34

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

公开(公告)号：US11795503B2

公开(公告)日：2023-10-24

申请号：US16512276

申请日：2019-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/6869 ,  G01N35/10 ,  B01L3/00 ,  G01N35/00 ,  G01N21/11 ,  B01F33/30 ,  B01F33/40 ,  B01J19/00 ,  C12Q1/6874 ,  G02B21/34 ,  G01N21/05

CPC classification number: C12Q1/6869 ,  B01F33/30 ,  B01F33/403 ,  B01J19/0046 ,  B01L3/50273 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N21/11 ,  G01N35/00069 ,  G01N35/0099 ,  G01N35/1002 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00466 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00869 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0851 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  G01N21/05 ,  G01N35/1011 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

公开(公告)号：US08889596B2

公开(公告)日：2014-11-18

申请号：US13969195

申请日：2013-08-16

Applicant: Life Technologies Corporation

Inventor： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC: C12Q1/68 ,  G01N35/10 ,  B01J19/00 ,  B01L3/00 ,  B01F13/00 ,  B01F13/02 ,  G01N35/00 ,  G02B21/34

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

Abstract translation: 根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。 洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。 洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。 在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。 在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。