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    Nucleic acids encoding neural axon outgrowth modulators
    5.
    发明授权
    Nucleic acids encoding neural axon outgrowth modulators 失效
    核酸编码神经轴突生长调节剂

    公开(公告)号：US5565331A

    公开(公告)日：1996-10-15

    申请号：US152019

    申请日：1993-11-12

    申请人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Merysia Placzek ,  Thomas Jessell ,  Jane Dodd

    发明人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Merysia Placzek ,  Thomas Jessell ,  Jane Dodd

    IPC分类号： A01K67/027 ,  A61K38/00 ,  A61P25/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/48 ,  C07K14/705 ,  C07K14/71 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  G01N33/53 ,  G01N33/68 ,  C12N15/00

    CPC分类号： G01N33/6896 ,  C07K14/4705 ,  C07K14/475 ,  C07K14/705 ,  C07K14/71 ,  A61K38/00 ,  G01N2500/04

    摘要： A novel classes of neural axon outgrowth promoting and orienting proteins, nucleic acids encoding such proteins and receptors which selectively bind such proteins are disclosed. The disclosed neural axon outgrowth promoting and orienting proteins include the laminin-related p75/p78 family; a family of vertebrate proteins which promote axon outgrowth and/or orientation, and p75/p78 family-specific receptors, including receptors found on spinal nerve axons, especially growth cones. Also disclosed are agents including peptides derived from the disclosed neural axon outgrowth promoting proteins capable of effecting axon outgrowth, orientation and regeneration. These agents provide small molecular weight modulators of nerve cell growth useful in the treatment of neurological disease and injury. The disclosed compositions also find use variously in screening chemical libraries for regulators of axon outgrowth and orientation, in genetic mapping, as probes for related genes, as diagnostic reagents for genetic neurological disease and in the production of specific cellular and animal systems for the development of neurological disease therapy.

    摘要翻译： 公开了一类促进和定向蛋白质的神经轴突生长，编码这种蛋白质的核酸和选择性结合这些蛋白质的受体。 所公开的神经轴突生长促进和定向蛋白质包括层粘连蛋白相关的p75 / p78家族; 促进轴突生长和/或取向的脊椎动物蛋白家族，以及p75 / p78家族特异性受体，包括在脊髓神经轴突，特别是生长锥上发现的受体。 还公开了包括衍生自所公开的神经轴突生长促进能够产生轴突向外生长，取向和再生的蛋白质的肽的试剂。 这些试剂提供了可用于治疗神经疾病和损伤的神经细胞生长的小分子量调节剂。 所公开的组合物还可用于筛选用于轴突生长和取向的调节剂的化学文库，遗传作图中作为相关基因的探针，作为遗传神经疾病的诊断试剂，以及用于开发特异性细胞和动物系统的生产 神经疾病治疗。

    Polynucleotide encoding human netrin-1
    6.
    发明授权
    Polynucleotide encoding human netrin-1 有权
    编码人netrin-1的多核苷酸

    公开(公告)号：US06218526B1

    公开(公告)日：2001-04-17

    申请号：US09136981

    申请日：1998-08-20

    申请人： Candace Swimmer ,  Anne Shyjan ,  David Leonardo ,  Yuan Zhang ,  Timothy Kennedy ,  Tito Serafini ,  Marc Tessier-Lavigne

    发明人： Candace Swimmer ,  Anne Shyjan ,  David Leonardo ,  Yuan Zhang ,  Timothy Kennedy ,  Tito Serafini ,  Marc Tessier-Lavigne

    IPC分类号： C12N1518

    CPC分类号： C07K14/475 ,  A61K38/00

    摘要： Specific netrin proteins, nucleic acids which encode netrin proteins and hybridization reagents, probes and primers capable of hybridizing with netrin genes and methods for screening chemical libraries for lead compounds for pharmacological agents useful in the diagnosis or treatment of disease associated undesirable cell growth are provided. An exemplary screen involves forming a mixture comprising a recombinant netrin protein, a natural intracellular netrin protein binding target, and a candidate pharmacological agent; incubating the mixture under conditions whereby, but for the presence of said candidate pharmacological agent, said netrin protein selectively binds said binding target; and detecting the presence or absence of specific binding of said netrin protein to said binding target.

    摘要翻译： 提供了特异性netrin蛋白质，编码netrin蛋白质的核酸和杂交试剂，能够与netrin基因杂交的探针和引物以及用于筛选用于诊断或治疗疾病相关的不希望的细胞生长的药物的铅化合物的化学文库的方法。 示例性屏幕包括形成包含重组netrin蛋白质，天然细胞内netrin蛋白结合靶标和候选药理学试剂的混合物; 在这样的条件下孵育混合物，但是对于所述候选药理学试剂的存在，所述网蛋白选择性结合所述结合靶; 并检测所述网蛋白与所述结合靶的特异性结合的存在或不存在。

    Human netrin-1
    7.
    发明授权
    Human netrin-1 失效
    人类netrin-1

    公开(公告)号：US5824775A

    公开(公告)日：1998-10-20

    申请号：US635137

    申请日：1996-04-19

    申请人： Candace Swimmer ,  Anne Shyjan ,  David Leonardo ,  Yuan Zhang ,  Timothy Kennedy ,  Tito Serafini ,  Marc Tessier-Lavigne

    发明人： Candace Swimmer ,  Anne Shyjan ,  David Leonardo ,  Yuan Zhang ,  Timothy Kennedy ,  Tito Serafini ,  Marc Tessier-Lavigne

    IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K38/00 ,  A61K45/00 ,  A61P25/00 ,  C07K14/47 ,  C07K14/475 ,  C12N15/18 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/566 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00

    CPC分类号： C07K14/475 ,  A61K38/00

    摘要： Specific netrin proteins, nucleic acids which encode netrin proteins and hybridization reagents, probes and primers capable of hybridizing with netrin genes and methods for screening chemical libraries for lead compounds for pharmacological agents useful in the diagnosis or treatment of disease associated undesirable cell growth are provided. An exemplary screen involves forming a mixture comprising a recombinant netrin protein, a natural intracellular netrin protein binding target, and a candidate pharmacological agent; incubating the mixture under conditions whereby, but for the presence of said candidate pharmacological agent, said netrin protein selectively binds said binding target; and detecting the presence or absence of specific binding of said netrin protein to said binding target.

    摘要翻译： 提供了特异性netrin蛋白质，编码netrin蛋白质的核酸和杂交试剂，能够与netrin基因杂交的探针和引物以及用于筛选用于诊断或治疗疾病相关的不希望的细胞生长的药物的铅化合物的化学文库的方法。 示例性屏幕包括形成包含重组netrin蛋白质，天然细胞内netrin蛋白结合靶标和候选药理学试剂的混合物; 在这样的条件下孵育混合物，但是对于所述候选药理学试剂的存在，所述网蛋白选择性结合所述结合靶; 并检测所述网蛋白与所述结合靶的特异性结合的存在或不存在。

    Method for isolating cell-type specific mrnas
    8.
    发明申请
    Method for isolating cell-type specific mrnas 有权
    分离细胞型特异性细胞的方法

    公开(公告)号：US20050009028A1

    公开(公告)日：2005-01-13

    申请号：US10494248

    申请日：2002-10-29

    申请人： Nathaniel Heintz ,  Tito Serafini ,  Andrew Shyjan

    发明人： Nathaniel Heintz ,  Tito Serafini ,  Andrew Shyjan

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N1/08 ,  C12P19/34

    CPC分类号： C12N15/1041 ,  C12N15/1006 ,  C12N15/82 ,  C12N15/8222 ,  C12Q1/6827

    摘要： The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA bound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.

    摘要翻译： 本发明提供了通过选择性分离以细胞类型特异性方式结合mRNA的核糖体或蛋白质分离细胞型特异性mRNA的方法，从而提供与结合mRNA的核糖体或蛋白质结合的mRNA。 作为核糖体复合物的核糖体结合正在细胞中积极翻译的mRNA。 根据本发明的方法，将细胞工程化以表达分子标记的核糖体蛋白质或蛋白质，其通过向细胞中引入核酸，所述核酸包含编码核糖体蛋白质或蛋白质的核苷酸序列，所述核糖体蛋白质或蛋白质与编码 肽标签。 然后可以分离标记的核糖体或mRNA结合蛋白以及与标记的核糖体或mRNA结合蛋白结合的mRNA，并分离mRNA并进一步用于基因表达分析。 本发明的方法促进了在异质细胞混合物中存在的所选细胞类型中的基因表达的分析和定量，而不需要将该细胞类型的细胞作为初步步骤分离。

    Method of defining cell types by probing comprehensive expression libraries with amplified RNA
    9.
    发明授权
    Method of defining cell types by probing comprehensive expression libraries with amplified RNA 失效
    通过用扩增的RNA探测综合表达文库来定义细胞类型的方法

    公开(公告)号：US6110711A

    公开(公告)日：2000-08-29

    申请号：US212338

    申请日：1998-12-15

    申请人： Tito Serafini ,  John Ngai

    发明人： Tito Serafini ,  John Ngai

    IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12N2517/02 ,  C12Q1/6897

    摘要： The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.

    摘要翻译： 本发明提供了用于定义细胞类型的方法和组合物，通常涉及以下步骤：（a）扩增异源细胞群体的单细胞的mRNA; （b）探测具有扩增的mRNA的综合表达文库以定义细胞的总表达谱; 和（c）将细胞的总表达谱与一个或多个其它细胞的总表达谱进行比较以限定细胞的唯一表达谱，其中细胞的唯一表达谱提供定义细胞类型的标记。

    Methods for making nucleic acids
    10.
    发明授权
    Methods for making nucleic acids 失效
    制备核酸的方法

    公开(公告)号：US06582936B1

    公开(公告)日：2003-06-24

    申请号：US09566570

    申请日：2000-05-08

    申请人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

    发明人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

    IPC分类号： C12P1934

    CPC分类号： C12Q1/6853 ,  C12N15/1096 ,  C12Q1/6809 ,  C12Q2531/143 ,  C12Q2525/173 ,  C12Q2525/125 ,  C12Q2525/107 ,  C12Q2525/191

    摘要： Nucleic acids are made by converting a primed single-stranded DNA to a double-stranded DNA by a method comprising the step contacting the single-stranded DNA with a DNA polymerase having 5′ exonuclease activity under conditions whereby the DNA polymerase converts the-single stranded DNA to the double-stranded DNA, wherein the single-stranded DNA is primed with oligonucleotide primer comprising a sequence complementary to the 3′ end of the single-stranded DNA, and at least one of the 5′ end of the primer and the single-stranded DNA comprises an RNA polymerase promoter joined to an upstream (5′) flanking moiety which protects the promoter from the 5′ exonuclease activity of the DNA polymerase.

    摘要翻译： 通过包括使单链DNA与具有5'外切核酸酶活性的DNA聚合酶接触的步骤的方法将引发的单链DNA转化为双链DNA来制备核酸，由此DNA聚合酶将单链DNA转化为单链DNA DNA到双链DNA，其中单链DNA用包含与单链DNA的3'末端互补的序列的寡核苷酸引物引物，并且引物的5'末端和单个DNA中的至少一个 标记的DNA包含连接到上游（5'）侧翼部分的RNA聚合酶启动子，其保护启动子不受DNA聚合酶的5'核酸外切酶活性的影响。