公开(公告)号：US6110711A

公开(公告)日：2000-08-29

申请号：US212338

申请日：1998-12-15

申请人： Tito Serafini ,  John Ngai

发明人： Tito Serafini ,  John Ngai

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12N2517/02 ,  C12Q1/6897

摘要： The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.

摘要翻译： 本发明提供了用于定义细胞类型的方法和组合物，通常涉及以下步骤：（a）扩增异源细胞群体的单细胞的mRNA; （b）探测具有扩增的mRNA的综合表达文库以定义细胞的总表达谱; 和（c）将细胞的总表达谱与一个或多个其它细胞的总表达谱进行比较以限定细胞的唯一表达谱，其中细胞的唯一表达谱提供定义细胞类型的标记。

公开(公告)号：US06582936B1

公开(公告)日：2003-06-24

申请号：US09566570

申请日：2000-05-08

申请人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

发明人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

IPC分类号： C12P1934

CPC分类号： C12Q1/6853 ,  C12N15/1096 ,  C12Q1/6809 ,  C12Q2531/143 ,  C12Q2525/173 ,  C12Q2525/125 ,  C12Q2525/107 ,  C12Q2525/191

摘要： Nucleic acids are made by converting a primed single-stranded DNA to a double-stranded DNA by a method comprising the step contacting the single-stranded DNA with a DNA polymerase having 5′ exonuclease activity under conditions whereby the DNA polymerase converts the-single stranded DNA to the double-stranded DNA, wherein the single-stranded DNA is primed with oligonucleotide primer comprising a sequence complementary to the 3′ end of the single-stranded DNA, and at least one of the 5′ end of the primer and the single-stranded DNA comprises an RNA polymerase promoter joined to an upstream (5′) flanking moiety which protects the promoter from the 5′ exonuclease activity of the DNA polymerase.

摘要翻译： 通过包括使单链DNA与具有5'外切核酸酶活性的DNA聚合酶接触的步骤的方法将引发的单链DNA转化为双链DNA来制备核酸，由此DNA聚合酶将单链DNA转化为单链DNA DNA到双链DNA，其中单链DNA用包含与单链DNA的3'末端互补的序列的寡核苷酸引物引物，并且引物的5'末端和单个DNA中的至少一个 标记的DNA包含连接到上游（5'）侧翼部分的RNA聚合酶启动子，其保护启动子不受DNA聚合酶的5'核酸外切酶活性的影响。

公开(公告)号：US06441269B1

公开(公告)日：2002-08-27

申请号：US09567637

申请日：2000-05-09

申请人： Tito Serafini ,  John Ngai

发明人： Tito Serafini ,  John Ngai

IPC分类号： C12N1500

CPC分类号： C12N15/1096 ,  C12N2517/02 ,  C12Q1/6809 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6897 ,  C12Q2565/518 ,  C12Q2531/143 ,  C12Q2525/191

摘要： The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type

摘要翻译： 本发明提供了用于定义细胞类型的方法和组合物，通常涉及以下步骤：（a）扩增异源细胞群体的单细胞的mRNA; （b）探测具有扩增的mRNA的综合表达文库以定义细胞的总表达谱; 和（c）将细胞的总表达谱与一个或多个其它细胞的总表达谱进行比较以定义细胞的唯一表达谱，其中细胞的唯一表达谱提供定义细胞类型的标记

公开(公告)号：US6114152A

公开(公告)日：2000-09-05

申请号：US49806

申请日：1998-03-27

申请人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

发明人： Tito Serafini ,  Percy Luu ,  John Ngai ,  David Lin

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12N15/1096 ,  C12Q1/6853

摘要： Nucleic acids are made by adding a known nucleotide sequence to the 3' end of a first RNA having a known sequence at the 5' end to form a second RNA and reverse transcribing the second RNA to form a cDNA. In one embodiment, the first RNA is an amplified mRNA, the known sequence at the 5' end comprises a poly(T) sequence, the adding step comprises using a polyadenyltransferase to add a poly(A) sequence to the 3' end, the reverse transcribing step is initiated at a duplex region comprising the poly(T) sequence hybridized to the poly(A) sequence, the cDNA is converted to double-stranded cDNA by a polymerase initiating from a noncovalently joined duplex region, and the double-stranded cDNA is transcribed to form one or more third RNAs.

摘要翻译： 通过向5'末端具有已知序列的第一个RNA的3'末端添加已知的核苷酸序列以形成第二个RNA并逆转录录第二个RNA以形成cDNA来制备核酸。 在一个实施方案中，第一RNA是扩增的mRNA，5'末端的已知序列包含poly（T）序列，所述添加步骤包括使用聚腺苷酸转移酶向3'端添加聚（A）序列， 在包含与poly（A）序列杂交的poly（T）序列的双链体区域开始逆转录步骤，通过从非共价连接的双链体区域起始的聚合酶将cDNA转化为双链cDNA，双链 cDNA被转录以形成一个或多个第三RNA。

公开(公告)号：US20050009028A1

公开(公告)日：2005-01-13

申请号：US10494248

申请日：2002-10-29

申请人： Nathaniel Heintz ,  Tito Serafini ,  Andrew Shyjan

发明人： Nathaniel Heintz ,  Tito Serafini ,  Andrew Shyjan

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N1/08 ,  C12P19/34

CPC分类号： C12N15/1041 ,  C12N15/1006 ,  C12N15/82 ,  C12N15/8222 ,  C12Q1/6827

摘要： The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA bound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.

摘要翻译： 本发明提供了通过选择性分离以细胞类型特异性方式结合mRNA的核糖体或蛋白质分离细胞型特异性mRNA的方法，从而提供与结合mRNA的核糖体或蛋白质结合的mRNA。 作为核糖体复合物的核糖体结合正在细胞中积极翻译的mRNA。 根据本发明的方法，将细胞工程化以表达分子标记的核糖体蛋白质或蛋白质，其通过向细胞中引入核酸，所述核酸包含编码核糖体蛋白质或蛋白质的核苷酸序列，所述核糖体蛋白质或蛋白质与编码 肽标签。 然后可以分离标记的核糖体或mRNA结合蛋白以及与标记的核糖体或mRNA结合蛋白结合的mRNA，并分离mRNA并进一步用于基因表达分析。 本发明的方法促进了在异质细胞混合物中存在的所选细胞类型中的基因表达的分析和定量，而不需要将该细胞类型的细胞作为初步步骤分离。

公开(公告)号：US5565331A

公开(公告)日：1996-10-15

申请号：US152019

申请日：1993-11-12

申请人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Merysia Placzek ,  Thomas Jessell ,  Jane Dodd

发明人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Merysia Placzek ,  Thomas Jessell ,  Jane Dodd

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61P25/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/48 ,  C07K14/705 ,  C07K14/71 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  G01N33/53 ,  G01N33/68 ,  C12N15/00

CPC分类号： G01N33/6896 ,  C07K14/4705 ,  C07K14/475 ,  C07K14/705 ,  C07K14/71 ,  A61K38/00 ,  G01N2500/04

摘要： A novel classes of neural axon outgrowth promoting and orienting proteins, nucleic acids encoding such proteins and receptors which selectively bind such proteins are disclosed. The disclosed neural axon outgrowth promoting and orienting proteins include the laminin-related p75/p78 family; a family of vertebrate proteins which promote axon outgrowth and/or orientation, and p75/p78 family-specific receptors, including receptors found on spinal nerve axons, especially growth cones. Also disclosed are agents including peptides derived from the disclosed neural axon outgrowth promoting proteins capable of effecting axon outgrowth, orientation and regeneration. These agents provide small molecular weight modulators of nerve cell growth useful in the treatment of neurological disease and injury. The disclosed compositions also find use variously in screening chemical libraries for regulators of axon outgrowth and orientation, in genetic mapping, as probes for related genes, as diagnostic reagents for genetic neurological disease and in the production of specific cellular and animal systems for the development of neurological disease therapy.

摘要翻译： 公开了一类促进和定向蛋白质的神经轴突生长，编码这种蛋白质的核酸和选择性结合这些蛋白质的受体。 所公开的神经轴突生长促进和定向蛋白质包括层粘连蛋白相关的p75 / p78家族; 促进轴突生长和/或取向的脊椎动物蛋白家族，以及p75 / p78家族特异性受体，包括在脊髓神经轴突，特别是生长锥上发现的受体。 还公开了包括衍生自所公开的神经轴突生长促进能够产生轴突向外生长，取向和再生的蛋白质的肽的试剂。 这些试剂提供了可用于治疗神经疾病和损伤的神经细胞生长的小分子量调节剂。 所公开的组合物还可用于筛选用于轴突生长和取向的调节剂的化学文库，遗传作图中作为相关基因的探针，作为遗传神经疾病的诊断试剂，以及用于开发特异性细胞和动物系统的生产 神经疾病治疗。

公开(公告)号：US06309638B1

公开(公告)日：2001-10-30

申请号：US09490517

申请日：2000-01-25

申请人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Marysia Placzek ,  Thomas Jessell ,  Jane Dodd

发明人： Marc Tessier-Lavigne ,  Tito Serafini ,  Timothy Kennedy ,  Marysia Placzek ,  Thomas Jessell ,  Jane Dodd

IPC分类号： A61K39395

CPC分类号： G01N33/6896 ,  A61K38/00 ,  C07K14/4705 ,  C07K14/475 ,  C07K14/705 ,  C07K14/71 ,  G01N2500/04

摘要： Netrin proteins, nucleic acids which encode netrin proteins and hybridization reagents, probes and primers capable of hybridizing with netrin genes and methods for screening chemical libraries for lead compounds for pharmacological agents are provided.

摘要翻译： 提供了Netrin蛋白质，编码netrin蛋白质的核酸和杂交试剂，能够与netrin基因杂交的探针和引物以及用于筛选用于药理学试剂的铅化合物化学文库的方法。

公开(公告)号：US20110136835A1

公开(公告)日：2011-06-09

申请号：US12882143

申请日：2010-09-14

申请人： Michael Kitt ,  Tito Serafini

发明人： Michael Kitt ,  Tito Serafini

IPC分类号： A61K31/519 ,  A61P19/06

CPC分类号： A61K45/06 ,  A61K31/167 ,  A61K31/195 ,  A61K31/426 ,  A61K31/519 ,  A61K31/7004 ,  A61K2300/00

摘要： Disclosed is a pharmaceutical composition comprising tranilast or a pharmaceutically acceptable salt thereof and allopurinol or a pharmaceutically acceptable salt thereof, wherein the amount by weight of said allopurinol or pharmaceutically acceptable salt thereof in said composition is greater than the amount by weight of said tranilast or pharmaceutically acceptable salt thereof in said composition.

摘要翻译： 公开了包含曲塞司特或其药学上可接受的盐和别嘌呤醇或其药学上可接受的盐的药物组合物，其中所述组合物中所述别嘌呤醇或其药学上可接受的盐的重量含量大于所述曲尼司特或药学上的重量 所述组合物中可接受的盐。

公开(公告)号：US20050266435A1

公开(公告)日：2005-12-01

申请号：US11076431

申请日：2005-03-09

申请人： David Hackos ,  Tito Serafini ,  Nina Orike

发明人： David Hackos ,  Tito Serafini ,  Nina Orike

IPC分类号： C07K14/705 ,  C07K16/28 ,  C12N15/12 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/567 ,  G01N33/68 ,  G01N33/94

CPC分类号： G01N33/6896 ,  C07K14/705 ,  G01N33/94 ,  G01N33/9486 ,  G01N2500/00 ,  G01N2800/2842

摘要： The present invention relates to nucleic and amino acid sequences encoding vanilloid receptors which are vanilloid-insensitive. The present invention provides mutant vanilloid receptors which are insensitive to vanilloid, but which are capable of responding to low pH, heat and other receptor modulators. The invention particularly provides mutant vanilloid insensitive human VR1 receptors. The invention also relates to methods and assays for screening for vanilloid receptor modulators that act independent of the vanilloid binding site and modulate receptor signals independent of a functional vanilloid, or capsaicin, response. The invention further provides methods of modulating the vanilloid receptor, independent of vanilloid response.

摘要翻译： 本发明涉及编码香草素不敏感的香草素受体的核酸和氨基酸序列。 本发明提供对香草素不敏感但能够对低pH，热等受体调节剂作出反应的突变型香草素受体。 本发明特别提供突变型香草素不敏感人VR1受体。 本发明还涉及用于筛选不依赖于香草素结合位点的香草素受体调节剂的方法和测定，并且调节与功能性香草素或辣椒素反应无关的受体信号。 本发明进一步提供调节香草素受体的方法，与香草素反应无关。

公开(公告)号：US20050215646A1

公开(公告)日：2005-09-29

申请号：US11076171

申请日：2005-03-08

申请人： Michael Kelly ,  Tito Serafini ,  Hang Chen

发明人： Michael Kelly ,  Tito Serafini ,  Hang Chen

IPC分类号： A61K31/15

CPC分类号： A61K31/15

摘要： Disclosed are pharmaceutical compositions for the treatment or prevention of chemokine mediated conditions, such as multiple sclerosis or related conditions, containing 3,4,5-trisubstituted aryl nitrones, and methods for the treatment or prevention of multiple sclerosis and related conditions. The 3,4,5-trisubstituted aryl nitrones have the formula: where R1-R3 and Q are as defined in the specification, or the 3,4,5-trisubstituted aryl nitrones have the formula: where R1-R3 and Q are as defined in the specification.

摘要翻译： 公开了用于治疗或预防包含3,4,5-三取代的芳基硝酮的趋化因子介导的病症例如多发性硬化或相关病症的药物组合物，以及治疗或预防多发性硬化症及相关病症的方法。 3,4,5-三取代的芳基硝酮具有下式：其中R 1 -R 3和Q如说明书中所定义，或3,4,5 三取代的芳基硝酮具有下式：其中R 1 -R 3和Q如说明书中所定义。