公开(公告)号：US20060127447A1

公开(公告)日：2006-06-15

申请号：US10520557

申请日：2003-07-10

申请人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Kwang Seok Kim ,  Jae Jun Song ,  Eu Gene Rha ,  Kwang Kim ,  Kenji Soda ,  Makoto Ashiuchi ,  Ha Ryoung Poo ,  Seung Lee

发明人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Kwang Seok Kim ,  Jae Jun Song ,  Eu Gene Rha ,  Kwang Kim ,  Kenji Soda ,  Makoto Ashiuchi ,  Ha Ryoung Poo ,  Seung Lee

IPC分类号： A61K38/16 ,  A61K47/00 ,  C07K14/32 ,  C12N1/21 ,  C12P21/06

CPC分类号： A61K9/0014 ,  A61K8/88 ,  A61K9/0095 ,  A61K47/42 ,  A61Q19/00 ,  C08H1/00 ,  C12P21/02

摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.

摘要翻译： 本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。 根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。 此外，它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。 根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料 在各种应用中。

公开(公告)号：US20060140971A1

公开(公告)日：2006-06-29

申请号：US10559631

申请日：2004-06-04

申请人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

发明人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

IPC分类号： A61K39/02 ,  C12N1/21 ,  C12N15/74 ,  C07K14/165

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/523 ,  C07K2319/00 ,  C12N9/93 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2770/20022 ,  Y02A50/476

摘要： The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

摘要翻译： 本发明涉及SARS冠状病毒抗原的表达表达载体，其含有编码SARS诱导冠状病毒抗原的基因和编码聚-γ-谷氨酸合酶复合物的pgsB，pgsC和pgsA基因中的一种或两种以上的基因，微生物转化 通过表面表达载体和包含该微生物的SARS疫苗。 根据本发明，可以在表面上使用表达SARS冠状病毒抗原的重组菌株经济地制备用于预防和治疗SARS的疫苗。

公开(公告)号：US07553636B2

公开(公告)日：2009-06-30

申请号：US10484605

申请日：2002-08-09

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Chul-Joong Kim ,  Kenji Soda ,  Makoto Ashiuchi

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Chul-Joong Kim ,  Kenji Soda ,  Makoto Ashiuchi

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N9/1051 ,  C07K2319/02 ,  C12N9/1288 ,  C12Y204/01133 ,  C12Y207/08005

摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

摘要翻译： 本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。 插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

公开(公告)号：US07323328B2

公开(公告)日：2008-01-29

申请号：US11186559

申请日：2005-07-21

申请人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

发明人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

IPC分类号： C12N1/20 ,  C12N9/88 ,  C12P39/00

CPC分类号： C12R1/01 ,  C12N9/88 ,  C12P13/227

摘要： The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebii SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

摘要翻译： 本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i））i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法，使用硝酸盐呼吸显示低生长产量的共生杆菌SC-1及其相对共生菌的生长方法，以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此，在本发明中由共生杆菌（Toibii）SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂，其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸（L-DOPA）和L-色氨酸。

公开(公告)号：US06967087B1

公开(公告)日：2005-11-22

申请号：US10220174

申请日：2000-07-14

申请人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

发明人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

IPC分类号： G01N33/569 ,  C12N1/20 ,  C12N9/88 ,  C12P13/22 ,  C12Q1/06 ,  C12R1/01 ,  G01N21/77 ,  C12P39/00 ,  C12Q1/12

CPC分类号： C12R1/01 ,  C12N9/88 ,  C12P13/227

摘要： The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

摘要翻译： 本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i））i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法，使用硝酸盐呼吸显示低生长量的共生杆菌SC-1及其相对共生菌的生长测定方法以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此，在本发明中由共生杆菌（Toibii）SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂，其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸（L-DOPA）和L-色氨酸。

公开(公告)号：US20070253935A1

公开(公告)日：2007-11-01

申请号：US11622158

申请日：2007-01-11

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahim ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahim ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： A61K39/00 ,  C12N1/21 ,  C12N15/00

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.

摘要翻译： 本发明涉及使用载体表达在微生物表面上表现出具有两亲性的肽抗生素P 5 3和Anal 3 35的各种抗细菌，抗真菌和抗癌活性61,63,65,67,69,71的方法， 含有源自芽孢杆菌属的外膜蛋白基因（pgsBCA）。 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及具有表面表达的肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，除了P 5 3和Anal 3 35之外，本发明可以应用于其他肽抗生素。

公开(公告)号：US07255855B2

公开(公告)日：2007-08-14

申请号：US10789164

申请日：2004-02-27

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： A01N63/00 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.

摘要翻译： 本发明涉及一种用于表达具有两亲性的肽抗生素P5 3和Ana1335的方法，并且在微生物表面上显示出抗菌，抗真菌和抗癌活性61,63,65,67,69,71，使用含有外源的载体 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各种肽抗生素P515和Ana1343的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，本发明可以应用于除P5 3和Ana1335之外的其它肽抗生素。

公开(公告)号：US20050191720A1

公开(公告)日：2005-09-01

申请号：US10789164

申请日：2004-02-27

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： C07H21/04 ,  C07K14/32 ,  C07K16/30 ,  C12N1/21 ,  C12N15/74 ,  C12P21/06

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.

摘要翻译： 本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法， 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，本发明可以应用于除P5 3和Anal335之外的其它肽抗生素。

公开(公告)号：US08080402B2

公开(公告)日：2011-12-20

申请号：US11622158

申请日：2007-01-11

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： C12N15/00 ,  C12N1/21 ,  A61K48/00

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal 3 35.

摘要翻译： 本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法， 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，除了P5 3和Anal 3 35之外，本发明可以应用于其它肽抗生素。

公开(公告)号：US08236940B2

公开(公告)日：2012-08-07

申请号：US12532605

申请日：2008-03-21

申请人： Moon Hee Sung ,  Chul Joong Kim ,  Seung-Pyo Hong ,  Haryoung Poo ,  Il Han Lee ,  Ji Yeon Kim ,  Kwang Kim

发明人： Moon Hee Sung ,  Chul Joong Kim ,  Seung-Pyo Hong ,  Haryoung Poo ,  Il Han Lee ,  Ji Yeon Kim ,  Kwang Kim

IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/00

CPC分类号： C12N15/746 ,  C12N9/1276

摘要： Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.

摘要翻译： 本文公开了醛缩酶基因启动子及其用途，更具体地，公开了源自干酪乳杆菌的醛缩酶基因的启动子，其具有SEQ ID NO：1的碱基序列，含有所述启动子的表达载体和转化的重组微生物 与所述表达载体。 用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质，因此可用作疫苗载体等。