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公开(公告)号:US07667194B2
公开(公告)日:2010-02-23
申请号:US11566351
申请日:2006-12-04
申请人: Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
发明人: Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
CPC分类号: B01J19/0046 , B01J2219/00378 , B01J2219/00529 , B01J2219/00533 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01L3/0268 , B01L2200/143 , B01L2400/0439 , C40B40/06 , C40B40/10 , C40B40/14 , C40B60/14 , G01N2035/1041
摘要: A method of producing a microarray including: (A) ejecting a liquid sample from an outlet onto an inspection carrier to form inspection spots, inspecting the resultant inspection spots for their quality to determine whether the inspected spots are defective or successful, and detecting a defective discharge unit, if any; (B) making the detected defective discharge unit stop discharging the liquid sample to prevent formation of the defective sample spot; (C) forming successful sample spots on a carrier using successful discharge units to provide a successful microarray on which the successful spots are aligned in a predetermined pattern on the carrier; and (D) forming a successful spot to be formed originally on the successful microarray at the position of the defective spot where no spot is formed in step (B).
摘要翻译: 一种生产微阵列的方法,包括:(A)将液体样品从出口喷射到检查载体上以形成检查点,检查所得检查点的质量,以确定检查点是否有缺陷或成功,并检测缺陷 排放单位(如有); (B)使检测到的有缺陷的排出单元停止排出液体样品以防止形成有缺陷的样品点; (C)使用成功的放电单元在载体上形成成功的样品点,以提供成功的斑点在载体上以预定图案对准的成功的微阵列; (D)在步骤(B)中在不形成斑点的缺陷点的位置上成功形成成功的微阵列的成功光斑。
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公开(公告)号:US07261862B2
公开(公告)日:2007-08-28
申请号:US10229910
申请日:2002-08-28
申请人: Motoo Noritake , Toshikazu Hirota , Takao Ohnishi
发明人: Motoo Noritake , Toshikazu Hirota , Takao Ohnishi
IPC分类号: B41J2/145
CPC分类号: B01L3/0268 , B01J2219/00369 , B01L2300/0819 , B01L2400/0439 , C40B60/14 , G01N2035/1039
摘要: An improved structure of a liquid drop emitter is provided which works to emit minute drops of liquid to deposit them in a dense array of dots on a substrate to produce a DNA chip, for example. The liquid drop emitter has a plurality of liquid inlets formed in rows in a staggered fashion, thereby allowing the liquid inlets to have a maximum possible area without any physical interference between adjacent two of them and also allowing outlet nozzles to be arranged in a dense array.
摘要翻译: 提供了一种液滴发射器的改进的结构,其工作是发射一滴液体,以将它们沉积在基底上的密集阵列中,以产生例如DNA芯片。 液滴发射器具有多个以交错方式成行的液体入口,从而允许液体入口具有最大可能的面积,而相邻的两个之间没有任何物理干扰,并且还允许出口喷嘴以致密的阵列 。
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公开(公告)号:US20070084997A1
公开(公告)日:2007-04-19
申请号:US11566351
申请日:2006-12-04
申请人: Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
发明人: Motoo Noritake , Takao Ohnishi , Toshikazu Hirota
IPC分类号: B01D59/44
CPC分类号: B01J19/0046 , B01J2219/00378 , B01J2219/00529 , B01J2219/00533 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00628 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00693 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01L3/0268 , B01L2200/143 , B01L2400/0439 , C40B40/06 , C40B40/10 , C40B40/14 , C40B60/14 , G01N2035/1041
摘要: A method of producing a microarray including: (A) ejecting a liquid sample from an outlet onto an inspection carrier to form inspection spots, inspecting the resultant inspection spots for their quality to determine whether the inspected spots are defective or successful, and detecting a defective discharge unit, if any; (B) making the detected defective discharge unit stop discharging the liquid sample to prevent formation of the defective sample spot; (C) forming successful sample spots on a carrier using successful discharge units to provide a successful microarray on which the successful spots are aligned in a predetermined pattern on the carrier; and (D) forming a successful spot to be formed originally on the successful microarray at the position of the defective spot where no spot is formed in step (B).
摘要翻译: 一种生产微阵列的方法,包括:(A)将液体样品从出口喷射到检查载体上以形成检查点,检查所得检查点的质量,以确定检查点是否有缺陷或成功,并检测缺陷 排放单位(如有); (B)使检测到的有缺陷的排出单元停止排出液体样品以防止形成有缺陷的样品点; (C)使用成功的放电单元在载体上形成成功的样品点,以提供成功的斑点在载体上以预定图案对准的成功的微阵列; (D)在步骤(B)中在不形成斑点的缺陷点的位置上成功形成成功的微阵列的成功光斑。
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公开(公告)号:US06365378B1
公开(公告)日:2002-04-02
申请号:US09694157
申请日:2000-10-23
申请人: Toshikazu Hirota , Motoo Noritake
发明人: Toshikazu Hirota , Motoo Noritake
IPC分类号: C12P1934
CPC分类号: B01L3/0268 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , B01L3/5088 , B01L7/52 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N35/00029 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要: A PCR product is prepared by PCR-amplifying a DNA fragment. The PCR product is then dried to prepare a DNA powder. The DNA powder is then charged into a sample-pouring port of each of the micropipettes of a dispenser. Subsequently, a buffer solution is poured from the sample-pouring port into a cavity to prepare a sample solution. After completion of the preparation of the sample solution in the cavity, an actuator section is driven to discharge and supply the sample solution onto a base plate.
摘要翻译: 通过PCR扩增DNA片段制备PCR产物。 然后将PCR产物干燥以制备DNA粉末。 然后将DNA粉末装入分配器的每个微量移液管的样品注入口中。 随后,将缓冲溶液从样品注入口倒入空腔中以制备样品溶液。 在空腔中准备样品溶液完成后,驱动致动器部分将样品溶液排放并提供到基板上。
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公开(公告)号:US06752326B2
公开(公告)日:2004-06-22
申请号:US09884457
申请日:2001-06-19
申请人: Toshikazu Hirota , Takao Ohnishi
发明人: Toshikazu Hirota , Takao Ohnishi
IPC分类号: B05B108
CPC分类号: F02M61/1846 , B05B17/0607 , F02M51/04 , F02M51/0603 , F02M57/021 , F02M57/027 , F02M61/166 , F02M61/168 , F02M61/18 , F02M61/1833 , F02M61/186 , F02M2200/90 , F02M2200/9007 , F02M2200/9015 , F02M2200/9038 , F02M2200/9046
摘要: The liquid droplet ejection apparatus includes a liquid supply path, a plurality of mutually independent pressurizing chambers, a plurality of liquid introduction bores for establishing communication between the corresponding pressurizing chambers and the liquid supply path, and a plurality of ejection nozzles for establishing communication between the corresponding pressurizing chambers and the exterior of the liquid droplet ejection apparatus. An ejection bore formed at the end portion of the ejection nozzle has a hollow cylindrical form and the inside diameter thereof increases toward an ejection opening. When a potential difference is applied between two electrodes of a piezoelectric/electrostrictive element, a ceramic sheet forming the upper wall of the pressurizing chamber deforms to thereby cause a change of the volume of the pressurizing chamber. Thus, liquid pressure within the pressurizing chamber increases to thereby cause simultaneous ejection of a plurality of liquid droplets from the ejection opening.
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公开(公告)号:US06465190B1
公开(公告)日:2002-10-15
申请号:US09694135
申请日:2000-10-23
IPC分类号: C12Q168
CPC分类号: G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要: Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译: 公开了一种方法,其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤,制备含有DNA片段的样品的样品制备步骤,稀释所得样品的浓度的稀释步骤, 以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。 样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤,将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤 。
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公开(公告)号:US07588322B2
公开(公告)日:2009-09-15
申请号:US11669608
申请日:2007-01-31
IPC分类号: B41J2/045
CPC分类号: B41J2/14209 , B41J2002/14217 , B41J2202/11
摘要: A liquid droplet discharging piezoelectric device 1 provided with a cavity member 11 with a built-in cavity 3; an introduction member 13 having introduction channel 5 connecting with the cavity 3; and a nozzle member 12 having nozzle channel 4 connecting with the cavity 3 on a side opposite to the channel 5. This liquid droplet discharging piezoelectric device 1 is provided with an introduction port 6, attached to the introduction member 13, capable of introducing a liquid into the cavity 3 via the introduction channel 5, and a discharge port 7, attached to the nozzle member 12, capable of discharging as droplets a liquid filled in the cavity 3 via the nozzle channel 40. Even in a case where an amount of liquid droplets is of a nanoliter (nl) order, excellent stability and reproducibility are attained, and the unit can stably be operated when attached to an apparatus.
摘要翻译: 具有内置空腔3的空腔构件11的液滴排出压电装置1; 引入构件13,其具有与空腔3连接的引入通道5; 以及喷嘴构件12,其具有在与通道5相反的一侧与空腔3连接的喷嘴通道4.该液滴排出压电装置1设置有引入口6,引入口6安装在导入构件13上,能够引入液体 通过引入通道5进入空腔3和附接到喷嘴构件12的排出口7,其能够通过喷嘴通道40作为液滴喷射填充在空腔3中的液体。即使在液体量 液滴具有纳升级(nl)级,达到优异的稳定性和再现性,并且当装置安装时该装置可以稳定地操作。
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公开(公告)号:US07160512B2
公开(公告)日:2007-01-09
申请号:US10135200
申请日:2002-04-30
申请人: Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
发明人: Toshikazu Hirota , Takao Ohnishi , Hiroyuki Tsuji
IPC分类号: B01L3/02
CPC分类号: B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00691 , B01J2219/00693 , B01J2219/00695 , B01J2219/00702 , B01J2219/00722 , B01L3/0241 , C40B40/06 , C40B60/14 , G01N35/109 , Y10T436/2575
摘要: A biochip manufacturing method can densely align spots of a plurality of samples in predetermined locations on a substrate using a discharge head equipped with one or more discharge modules composed of one or more discharge units, and a first moving table on which one or more trays with one or more substrates fixed thereon are detachably mounted. According to the biochip manufacturing method, the high precision in the operation for densely aligning and fixing droplets with a minute volume on a predetermined substrate (micro spot-forming operation) can be attained, and the time required for the micro spot-forming operation can be shortened as well.
摘要翻译: 生物芯片制造方法可以使用配备有由一个或多个排出单元组成的一个或多个排出模块的排出头以及第一移动工作台,将一个或多个具有 固定在其上的一个或多个基板可拆卸地安装。 根据生物芯片的制造方法,可以实现在规定的基板上微小对准和固定微小的液滴的操作的高精度(微点形成操作),并且微点成形操作所需的时间可以 也缩短了。
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公开(公告)号:US06875402B2
公开(公告)日:2005-04-05
申请号:US09977567
申请日:2001-10-15
申请人: Toshikazu Hirota , Takao Ohnishi
发明人: Toshikazu Hirota , Takao Ohnishi
CPC分类号: B01L3/0268 , B01J19/0046 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N2035/1041 , Y10S73/04 , Y10T436/2575
摘要: A micropipette usable for producing a biochip inclusive of DNA micro arrays capable of arraying and fixing droplets of a micro-volume on a substrate in a high density includes a main body, at least one cavity for storing a sample, at least one ejection port, a piezoelectic/electrostrictive element mounted on the outer surface of the main body, and at least one sample inlet port for supplying sample from the outside. A sample that is temporarily stored in the cavity is discharged from at least one ejection port, by virtue of the movement of the piezoelectric/electrostrictive element, to outside of the micropipette via a through hole in a nozzle portion formed in the pipette main body. The nozzle portion through hole has three or more projection radially protruding from the center of the through hole and having a specific shape.
摘要翻译: 可用于生产包括能够以高密度排列和固定微量体积的液滴的DNA微阵列的生物芯片的微量移液器包括主体,至少一个用于存储样品的空腔,至少一个排出口, 安装在主体的外表面上的压电/电致伸缩元件和用于从外部供应样品的至少一个样品入口。 通过压电/电致伸缩元件的移动,临时存储在空腔中的样品通过形成在移液管主体中的喷嘴部分中的通孔从至少一个喷射口排出到微量移液管外部。 喷嘴部通孔具有从通孔的中心径向突出并具有特定形状的三个以上的突起。
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公开(公告)号:US06753144B1
公开(公告)日:2004-06-22
申请号:US09868832
申请日:2001-06-21
申请人: Toshikazu Hirota , Takao Ohnishi
发明人: Toshikazu Hirota , Takao Ohnishi
IPC分类号: C12Q168
CPC分类号: G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0241 , B01L3/0268 , B01L3/5085 , B01L3/5088 , B01L9/54 , B01L2200/021 , B01L2200/027 , B01L2300/0816 , B01L2300/0819 , B01L2400/027 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , Y10T436/25 , Y10T436/2575 , C12Q2565/507
摘要: When genetic analyses are performed using the DNA microarray of the present invention, the inspection accuracy is improved. A sample solution is supplied onto a base plate 10 to prepare the DNA microarray 20 which includes a large number of spots 80 containing capture solutions arranged on the base plate 10. The capture solutions are adapted to specifically react with a specimen and provide information about a structure within the specimen. In the microarray 20, the planar configuration of the spots 80 are substantially circular, and a plurality of spots having different spot sizes are formed on the base plate.
摘要翻译: 当使用本发明的DNA微阵列进行遗传分析时,提高了检查精度。 将样品溶液供应到基板10上以制备包括大量含有布置在基板10上的捕获溶液的斑点80的DNA微阵列20.捕获溶液适于与样品特异性反应并提供关于 标本内的结构。 在微阵列20中,斑点80的平面构造基本上是圆形的,并且在基板上形成具有不同光斑尺寸的多个斑点。
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