摘要:
A method of producing a microarray including: (A) ejecting a liquid sample from an outlet onto an inspection carrier to form inspection spots, inspecting the resultant inspection spots for their quality to determine whether the inspected spots are defective or successful, and detecting a defective discharge unit, if any; (B) making the detected defective discharge unit stop discharging the liquid sample to prevent formation of the defective sample spot; (C) forming successful sample spots on a carrier using successful discharge units to provide a successful microarray on which the successful spots are aligned in a predetermined pattern on the carrier; and (D) forming a successful spot to be formed originally on the successful microarray at the position of the defective spot where no spot is formed in step (B).
摘要:
A PCR product is prepared by PCR-amplifying a DNA fragment. The PCR product is then dried to prepare a DNA powder. The DNA powder is then charged into a sample-pouring port of each of the micropipettes of a dispenser. Subsequently, a buffer solution is poured from the sample-pouring port into a cavity to prepare a sample solution. After completion of the preparation of the sample solution in the cavity, an actuator section is driven to discharge and supply the sample solution onto a base plate.
摘要:
A method of producing a microarray including: (A) ejecting a liquid sample from an outlet onto an inspection carrier to form inspection spots, inspecting the resultant inspection spots for their quality to determine whether the inspected spots are defective or successful, and detecting a defective discharge unit, if any; (B) making the detected defective discharge unit stop discharging the liquid sample to prevent formation of the defective sample spot; (C) forming successful sample spots on a carrier using successful discharge units to provide a successful microarray on which the successful spots are aligned in a predetermined pattern on the carrier; and (D) forming a successful spot to be formed originally on the successful microarray at the position of the defective spot where no spot is formed in step (B).
摘要:
An improved structure of a liquid drop emitter is provided which works to emit minute drops of liquid to deposit them in a dense array of dots on a substrate to produce a DNA chip, for example. The liquid drop emitter has a plurality of liquid inlets formed in rows in a staggered fashion, thereby allowing the liquid inlets to have a maximum possible area without any physical interference between adjacent two of them and also allowing outlet nozzles to be arranged in a dense array.
摘要:
The liquid droplet ejection apparatus includes a liquid supply path, a plurality of mutually independent pressurizing chambers, a plurality of liquid introduction bores for establishing communication between the corresponding pressurizing chambers and the liquid supply path, and a plurality of ejection nozzles for establishing communication between the corresponding pressurizing chambers and the exterior of the liquid droplet ejection apparatus. An ejection bore formed at the end portion of the ejection nozzle has a hollow cylindrical form and the inside diameter thereof increases toward an ejection opening. When a potential difference is applied between two electrodes of a piezoelectric/electrostrictive element, a ceramic sheet forming the upper wall of the pressurizing chamber deforms to thereby cause a change of the volume of the pressurizing chamber. Thus, liquid pressure within the pressurizing chamber increases to thereby cause simultaneous ejection of a plurality of liquid droplets from the ejection opening.
摘要:
Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要:
A method for detecting the solid particles contained in a fluid, by the use of a particle sensor having a flow path of a fluid, having a fluid inlet and a fluid outlet, and a sensor element provided in the flow path, which comprises (a) a vibrating section having such a mass that is sensitive to the collision of solid particles contained in the fluid, with the vibrating section and (b) a detecting section for detecting the vibration of the vibrating section caused by the collision and converting the vibration to electric signals. The method includes steps of: passing a fluid to be examined, through the particle sensor, selectively detecting, from the electric signals outputted from the particle sensor, an electric signal portion having particular frequencies ranging from 1/50 to 10 times the primary resonance frequency of the sensor element, measuring the maximum amplitude of the electric signal portion, comparing the maximum amplitude measured above, with a given value predetermined with the particle sensor based on (1) the maximum amplitude of a portion of the electric signals caused by the collision of solid particles contained in a fluid, having the above particular frequencies and (2) the maximum amplitude of a portion of the electric signals caused by the turbulent flow of the same fluid containing no solid particles, having the above particular frequencies, and judging, depending upon the result of the above comparison, whether or not the electric signals outputted from the particle sensor with the fluid to be examined have been caused by the collision of solid particles with the particle sensor.
摘要:
A recording head operable to apply an electric current to an electrically resistive layer provided on a recording medium or a planar intermediate member interposed between the medium and the recording head. The head includes an electrically insulating substrate, and at least one recording electrode formed on one of opposite major surfaces of the substrate. The substrate is formed of an electrically insulating material whose wear resistance is lower than that of the recording electrode or electrodes. The substrate has a distal end portion extending from a proximal portion by a predetermined distance from the proximal portion for contact with the electrically resistive layer. The distal end portion has a thickness smaller than that of the proximal portion, as measured in a direction perpendicular to a direction of extension of the distal end portion. At least one return circuit electrode formed from a metal or metal alloy sheet is provided on the other major surface of the substrate remote from the recording electrode(s).
摘要:
Disclosed are a carrier that carries identifying information having favorable identification performance and a use thereof. The carrier carries identifying information for identifying an identification subject, and comprises one or more pieces of DNA having a thymine-rich base sequence and having, as the identifying information, an identifying base sequence pre-associated with the identification subject.
摘要:
A liquid droplet discharging piezoelectric device 1 provided with a cavity member 11 with a built-in cavity 3; an introduction member 13 having introduction channel 5 connecting with the cavity 3; and a nozzle member 12 having nozzle channel 4 connecting with the cavity 3 on a side opposite to the channel 5. This liquid droplet discharging piezoelectric device 1 is provided with an introduction port 6, attached to the introduction member 13, capable of introducing a liquid into the cavity 3 via the introduction channel 5, and a discharge port 7, attached to the nozzle member 12, capable of discharging as droplets a liquid filled in the cavity 3 via the nozzle channel 40. Even in a case where an amount of liquid droplets is of a nanoliter (nl) order, excellent stability and reproducibility are attained, and the unit can stably be operated when attached to an apparatus.