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    Magnetic particles, method for obtaining same and uses for separating molecules
    6.
    发明授权
    Magnetic particles, method for obtaining same and uses for separating molecules 有权
    磁性粒子，其获得方法和用于分离分子的方法

    公开(公告)号：US06521341B1

    公开(公告)日：2003-02-18

    申请号：US09582352

    申请日：2000-09-21

    申请人： Abdelhamid Elaissari ,  Christian Pichot ,  Bernard Mandrand

    发明人： Abdelhamid Elaissari ,  Christian Pichot ,  Bernard Mandrand

    IPC分类号： G11B516

    CPC分类号： H01F1/111 ,  B03C1/01 ,  G01N33/5434 ,  G01N2446/20 ,  G01N2446/86 ,  Y10S428/90 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： The invention concerns magnetic and heat-sensitive particles having a predetermined size between 0.05 and 10 &mgr;m, each particle comprising: an inner composite core consisting of a solid organic or inorganic particle, containing within it a magnetic filler; an outer coati based on a polymer capable of interacting with at least a biological molecule, the outer polymer is heat-sensitive and has a predetermined low critical solubility temperature (LCST) ranging between 10 and 100° C. and preferably between 20 and 60° C. The invention also concerns methods for obtaining said particles and their uses. The particles are characterized in that there is an intermediate layer between the inner core and the outer layer isolating said inner core magnetic filler with respect to said functionalized outer layer. Said invention is particularly useful for separating proteins and/or nucleic acids.

    摘要翻译： 本发明涉及具有0.05-10μm之间的预定尺寸的磁性和热敏颗粒，每个颗粒包括：由固体有机或无机颗粒组成的内部复合芯，其中含有磁性填料; 基于能够与至少一种生物分子相互作用的聚合物的外涂层，外部聚合物是热敏感的，并且具有在10和100℃之间，优选在20和60℃之间的预定的低临界溶解温度（LCST） 本发明还涉及获得所述颗粒及其用途的方法。 颗粒的特征在于，在内芯和外层之间存在相对于所述官能化外层隔离所述内芯磁性填料的中间层。 所述发明对于分离蛋白质和/或核酸特别有用。

    Nucleic acid isolation
    7.
    发明授权
    Nucleic acid isolation 失效
    核酸分离

    公开(公告)号：US06737235B1

    公开(公告)日：2004-05-18

    申请号：US08945731

    申请日：1997-11-10

    申请人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    发明人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    IPC分类号： C07H2104

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

    摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2 M is achieved.

    摘要翻译： 公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。 该方法包括一种吸附剂制备步骤（a），其包括由水性连续相组成的溶胶和包含通过聚合（1）第一水溶性丙烯酰胺或丙烯酰胺衍生物单体制备的官能化颗粒聚合物的分散颗粒基质相（ 2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解度温度（LCST）。 接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。

    Device for the capture of target molecules, and capturing process using the device
    8.
    发明授权
    Device for the capture of target molecules, and capturing process using the device 失效
    用于捕获目标分子的装置，以及使用该装置的捕获过程

    公开(公告)号：US5723344A

    公开(公告)日：1998-03-03

    申请号：US264996

    申请日：1994-06-24

    申请人： Claude Mabilat ,  Philippe Cros ,  Bernard Mandrand ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    发明人： Claude Mabilat ,  Philippe Cros ,  Bernard Mandrand ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N33/544

    CPC分类号： G01N33/54353 ,  G01N33/54366 ,  Y10S435/975 ,  Y10S436/807

    摘要： Device for capturing a target molecule for the purpose of detecting it and/or assaying it, including a solid support on which is immobilized a ligand, the ligand being provided in the form of a conjugate resulting from the covalent coupling of a polymer with a plurality of molecules of the ligand. The polymer is an N-vinylpyrrolidone copolymer, and the conjugate is immobilized on the solid support by adsorption. When the ligand is capable of forming a complex with the target, the device is specific for a given target. When the device comprises, in addition, a bifunctional reagent capable of forming a complex, on the one hand, with the ligand and, on the other hand, with the target, the support on which the ligand is immobilized constitutes a universal capturing system.

    摘要翻译： 用于捕获靶分子以用于检测和/或测定靶分子的装置，包括其上固定有配体的固体支持体，所述配体以由聚合物与多个共价键共价偶联得到的缀合物形式提供 的配体分子。 聚合物是N-乙烯基吡咯烷酮共聚物，并且通过吸附将缀合物固定在固体载体上。 当配体能够与目标物形成复合物时，该装置对于给定的靶标是特异性的。 此外，当该装置另外包含能够与配体一起形成络合物的双功能试剂，另一方面，另外，与靶标配位的配体固定的载体构成通用捕获系统。

    Reagent and method for the detection of a nucleotide sequence with signal amplification
    9.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US6017707A

    公开(公告)日：2000-01-25

    申请号：US870730

    申请日：1997-06-06

    申请人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    发明人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC分类号： C08G81/02 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    摘要翻译： 检测目的核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有 包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

    Reagent and method for the detection of a nucleotide sequence with signal amplification
    10.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US5695936A

    公开(公告)日：1997-12-09

    申请号：US433505

    申请日：1995-06-14

    申请人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    发明人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC分类号： C08G81/02 ,  C12Q1/68

    CPC分类号： C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    摘要翻译： PCT No.PCT / FR94 / 01084第 371 1995年6月14日第 102（e）1995年6月14日PCT PCT 1994年9月15日PCT公布。 WO95 / 08000 PCT出版物 1995年3月23日的方法检测目标核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括使用基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分 具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。