摘要:
The invention concerns an analysis device including at least one chip (110) equipped with a plurality of analysis electrodes (112). In accordance with the invention, the device also includes means of individual heating (150) of the analysis electrodes. The invention can be used for the analysis of chemical or biological products, for example in an antigen/antibody recognition or DNA/DNA analysis.
摘要:
A method and device for testing an electronic chip (10) having on the surface a plurality of addressable electrically conductive sensitive elements (12) in which the sensitive elements on the chip are put in contact with a conductive solution (52), one or more sensitive elements (12) are addressed selectively in order to apply to each sensitive element addressed an electrical test signal referred to as an input signal, and a signal, referred to as the output signal, on an electrode (60, 60a) also in contact with the conductive solution, is measured.
摘要:
The invention concerns a method for fabricating matrices of addressed ligands on a carrier. According to this method, an element is used such as a reservoir (1) filled with ligand and containing an electrode (3) to deposit and electrochemically fix the ligand to the conductive carrier (7). The ligand may be an oligonucleotide or a peptide, and fixing may be obtained by electropolymerisation of this oligonucleotide or peptide carrying a pyrrole group at 5′ with pyrrole.
摘要:
The present invention relates to polysaccharides selected from sulphated arabinogalactans, apiogalacturonans and sulphated heteroglycans intended to be used as a drug for the preventive or curative treatment of an influenza virus, as well as to the pharmaceutical compositions including, in particular in combination with at least one pharmaceutically acceptable carrier: either an extract of Codium fragile, including sulphated arabinogalactans, or an extract of Zostera marina or Lemna minor, including apiogalacturonans, or an extract of Caulerpa racemosa, including sulphated heteroglycans.
摘要:
The invention relates to a device for receiving a fluid sample, which is designed such as to form an electrode, such as a counter electrode or a working electrode, in an electrochemical cell. The inventive device comprises an end part having at least one cavity which opens to the exterior via an opening and which is equipped with a base. The invention is characterized in that the aforementioned end part comprises a first electrically-insulating hydrophobic zone which is adjacent to the cavity opening and a second electrically-conducting hydrophilic zone which is adjacent to the first zone and which at least partially covers the base of the cavity, such that, when the end part is immersed in the fluid and then removed therefrom, the cavity retains part of the fluid by means of capillary action.
摘要:
A method for detecting specific nucleic acid sequences (a single sequence and/or a mixture of nucleic acid sequences) which are present in a biological sample, comprising at least one enzymatic amplification. Detection is carried out, after placing the biological samples in a solution in order to extract the nucleic acid, by enrichment with target sequences by putting the biological samples into contact with at least one pair of appropriate triggers, followed by at least one appropriate dilution of the obtained enrichment amplifying solution, and by putting a fraction of this solution into contact with at least one pair of triggers, followed by the detection of the obtained double-strand target nucleic acid copies. Applications in the diagnosis of genetic, infectious and tumoral diseases, in checking biological samples and in cell typing etc.
摘要:
The present invention relates to a method for real-time measurement of the secretion of at least one compound by at least one individual cell, comprising: the culturing, in a liquid medium, of at least one cell in a culture chamber, at least one wall of which comprises at least one sensitive area, a sensitive area comprising a plurality of ligands, attached to a solid support, each ligand being able to bind specifically to the compound, and an element for real-time transduction of a signal produced by the binding of the compound to one of the ligands; the identification, in a sensitive area, of at least one spot producing a signal; the real-time measurement of the signal produced by the spot identified, representing the amount of compound secreted by an individual cell.
摘要:
The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip.This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps: coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound, preparation of a second solution of pyrrole not containing the protein, mixing of said first solution with said second solution so as to obtain an electropolymerization solution, electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.
摘要:
A method for fabricating matrices of addressed ligands on a carrier. In the method, an element is used such as a reservoir filled with ligand and containing an electrode to deposit and electrochemically fix the ligand to the conductive carrier. The ligand may be an oligonucleotide or a peptide, and fixing may be obtained by electrocopolymerisation of this oligonucleotide or peptide carrying a pyrrole group at 5′ with pyrrole.
摘要:
A method for preparing double stranded RNA, wherein the simultaneous transcription of two complementary DNA sequence strands in a single reaction mixture is preformed, and the two resulting transcripts are immediately hybridized. The double stranded RNA fragments may be used for detecting and/or assaying nucleic acid target sequences in a biological sample.