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    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    3.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 有权
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：US20100273219A1

    公开(公告)日：2010-10-28

    申请号：US12753703

    申请日：2010-04-02

    申请人： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    发明人： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    摘要： In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    摘要翻译： 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

    Patterned chip with adjustable surface and method for manufacturing the same
    5.
    发明申请
    Patterned chip with adjustable surface and method for manufacturing the same 审中-公开
    具有可调表面的图案化芯片及其制造方法

    公开(公告)号：US20070235333A1

    公开(公告)日：2007-10-11

    申请号：US11400507

    申请日：2006-04-07

    申请人： Peilin Chen ,  Jau-Ye Shiu ,  Chiung-Wen Kuo

    发明人： Peilin Chen ,  Jau-Ye Shiu ,  Chiung-Wen Kuo

    IPC分类号： C07K1/26

    CPC分类号： C07K1/1077 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y30/00

    摘要： The invention is to provide a method for fabricating a patterned chip with adjustable surface including of a basic layer, an insulating layer and a layer of biomolecules or cells. The method comprises of the following steps: pre-patterning a basic layer by photolithography; coating the pre-patterned basic layer with an insulating layer, the insulating layer is placed in solution containing biomolecules or cells with external voltage applied to the insulating layer. In addition, two steps—fabricating an pattern on the insulating layer matching the pattern of the basic layer by photolithography and treating the patterned insulating surface with chemical, physical or plasma surface process treatment—can be further followed by the step of pre-patterning a basic layer by photolithography, then proceeding the remaining steps.

    摘要翻译： 本发明提供一种用于制造具有包括基本层，绝缘层和生物分子或细胞层的具有可调表面的图案化芯片的方法。 该方法包括以下步骤：通过光刻预先图案化基底层; 用绝缘层涂覆预图案化的基底层，将绝缘层放置在含有施加到绝缘层的外部电压的生物分子或电池的溶液中。 另外，通过光刻法在匹配基底层的图案的绝缘层上制造图案并用化学，物理或等离子体表面处理处理来处理图案化绝缘表面的两个步骤可以进一步遵循以下步骤：预图案化 基础层通过光刻，然后进行剩余的步骤。

    Electrically controlled addressable multi-dimensional microfluidic device and method
    6.
    发明申请
    Electrically controlled addressable multi-dimensional microfluidic device and method 审中-公开
    电控可寻址多维微流体装置及方法

    公开(公告)号：US20070095407A1

    公开(公告)日：2007-05-03

    申请号：US11262495

    申请日：2005-10-28

    申请人： Peilin Chen ,  Jau-Ye Shiu ,  Chiung-Wen Kuo

    发明人： Peilin Chen ,  Jau-Ye Shiu ,  Chiung-Wen Kuo

    IPC分类号： F16K11/20

    CPC分类号： G02B26/004 ,  B01L3/502707 ,  B01L3/50273 ,  B01L9/52 ,  B01L13/02 ,  B01L2300/0819 ,  B01L2300/0874 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0487 ,  G09G3/3433 ,  Y10T137/87249

    摘要： A microfluidic device that includes one or more microchannels configured to transport fluid, and one or more microchambers configured to receive the fluid, wherein each of the one or more microchannels is coupled in fluid communication to at least one of the one or more microchambers. The microfluidic device also includes a first set of electrodes, each of the electrodes electrically coupled to one of the one or more microchannels, and configured to selectively apply an adjustable voltage to the respective microchannel to cause the fluid in that microchannel to flow. The microfluidic device further includes a second set of electrodes, where each of the electrodes in the second set of electrodes is electrically coupled to corresponding microchambers and configured to apply an adjustable voltage to the corresponding microchambers to direct flowing fluid into those corresponding microchambers from the microchannels to which those corresponding microchambers are coupled.

    摘要翻译： 一种微流体装置，其包括构造成输送流体的一个或多个微通道，以及被配置为接收流体的一个或多个微室，其中所述一个或多个微通道中的每一个与所述一个或多个微室中的至少一个流体连通地耦合。 微流体装置还包括第一组电极，每个电极电耦合到一个或多个微通道之一，并且被配置为选择性地将可调电压施加到相应的微通道以使该微通道中的流体流动。 微流体装置还包括第二组电极，其中第二组电极中的每个电极电耦合到对应的微室，并且被配置为向相应的微室施加可调节的电压，以将流动流体引导到来自微通道的相应微室中 那些相应的微室耦合到这些微室。

    Nucleic acid encoding reactions
    7.
    发明授权
    Nucleic acid encoding reactions 有权
    核酸编码反应

    公开(公告)号：US09074204B2

    公开(公告)日：2015-07-07

    申请号：US13476911

    申请日：2012-05-21

    申请人： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    发明人： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/65 ,  C12N15/66

    CPC分类号： C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    摘要： Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    摘要翻译： 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    NUCLEIC ACID ENCODING REACTIONS
    8.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 有权
    核酸编码反应

    公开(公告)号：US20130005585A1

    公开(公告)日：2013-01-03

    申请号：US13476911

    申请日：2012-05-21

    申请人： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    发明人： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC分类号： C40B20/00 ,  C12Q1/68 ,  C40B30/04 ,  C12P19/34 ,  C40B50/06 ,  C40B40/06

    CPC分类号： C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    摘要： Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    摘要翻译： 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。