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    Orthogonal translation components for the in vivo incorporation of unnatural amino acids
    4.
    发明授权
    Orthogonal translation components for the in vivo incorporation of unnatural amino acids 有权
    用于体内引入非天然氨基酸的正交翻译组分

    公开(公告)号：US08216804B2

    公开(公告)日：2012-07-10

    申请号：US11665083

    申请日：2005-10-27

    申请人： Peter G. Schultz ,  Jianming Xie ,  Huaqiang Zeng

    发明人： Peter G. Schultz ,  Jianming Xie ,  Huaqiang Zeng

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N1/19

    CPC分类号： C12N9/93 ,  C12N15/1058 ,  C12P21/00 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸掺入到在细菌宿主细胞如大肠杆菌或真核宿主如酵母细胞中产生的蛋白质中。 本发明提供了例如但不限于新的正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸的蛋白质的方法和翻译系统。

    GENETICALLY ENCODED FLUORESCENT COUMARIN AMINO ACIDS
    5.
    发明申请
    GENETICALLY ENCODED FLUORESCENT COUMARIN AMINO ACIDS 审中-公开
    基因编码荧光蛋白氨基酸

    公开(公告)号：US20120329162A1

    公开(公告)日：2012-12-27

    申请号：US13460174

    申请日：2012-04-30

    申请人： Jiangyun Wang ,  Jianming Xie ,  Peter G. Schultz

    发明人： Jiangyun Wang ,  Jianming Xie ,  Peter G. Schultz

    IPC分类号： C07K14/00 ,  C12P21/02 ,  G01N21/64 ,  C12N9/00

    CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/10 ,  C12N15/67

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将香豆素非天然氨基酸L-（7-羟基香豆素-4-基）乙基甘氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸L-（7-羟基香豆素-4-基）乙基甘氨酸的蛋白质的方法和相关翻译系统。

    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
    7.
    发明授权
    Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination 失效
    将重原子含有非天然氨基酸的位点特异性并入蛋白质进行结构测定

    公开(公告)号：US07399619B2

    公开(公告)日：2008-07-15

    申请号：US11137850

    申请日：2005-05-24

    申请人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    发明人： Jianming Xie ,  Lei Wang ,  Ning Wu ,  Peter G. Schultz

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/00 ,  C12N1/21 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

    CPC分类号： C12N9/93 ,  C07H21/04 ,  C12N15/67 ,  C12P21/02

    摘要： Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

    摘要翻译： 提供了翻译系统和包括正交氨酰基tRNA合成酶的其它组合物，其优先用碘化或溴化氨基酸对正交的tRNA进行充电。 还描述了编码这种合成酶的核酸，以及用于产生蛋白质的方法和试剂盒，包括含重原子的氨基酸，例如溴化或碘化​​氨基酸。 还描述了确定蛋白质结构的方法，例如通过使用正交的tRNA /氨基酰基tRNA-合成酶对将重原子特异性并入的蛋白质的蛋白质。