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1.发明授权公开(公告)号：US07399605B2
公开(公告)日：2008-07-15
申请号：US10085476
申请日：2002-02-27
CPC分类号： C12N9/1241 , C12N9/127 , C12N2799/026
摘要： The present invention relates to the molecular biology and virology of the hepatitis C virus (HCV). An object of the present invention is a method to reproduce in vitro the RNA-dependent RNA polymerase activity of HCV that makes use of sequences contained in the HCV NS5B protein.
摘要翻译： 本发明涉及丙型肝炎病毒（HCV）的分子生物学和病毒学。 本发明的一个目的是在体外再现利用HCV NS5B蛋白中包含的序列的HCV的RNA依赖性RNA聚合酶活性的方法。
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2.发明授权Method for producing in vitro the RNA-dependent RNA polymerase and terminal nucleotidyl transferase activities encoded by hepatitis C virus (HCV) 失效用于在体外生产RNA依赖性RNA聚合酶和由丙型肝炎病毒（HCV）编码的末端核苷酸转移酶活性的方法公开(公告)号：US06383768B1
公开(公告)日：2002-05-07
申请号：US08952981
申请日：1998-03-23
IPC分类号： C12Q148
CPC分类号： C12N9/1241 , C12N9/127 , C12N2799/026
摘要： This is a method for reproducing in vitro the RNA-dependent RNA polymerase activity associated with hepatitis C virus. The method is characterized in that sequences contained in NS5B are used in the reaction mixture. The terminal nucleotidyl transferase activity, a further property of the NS5B protein, can also be reproduced using this method. The method takes advantage of the fact that the NS5B protein, either purified to apparent homogeneity or present in extracts of overproducing organisms, can catalyze the addition of ribonucleotides to the 3′-termini of exogenous or endogenous RNA molecules. The invention also relates to a composition of matter that comprises sequences contained in NS5B, and to the use of these compositions for the set up of an enzymatic test capable of selecting, for therapeutic purposes, compounds that inhibit the enzymatic activity associated with NS5B.
摘要翻译： 这是一种在体外再生与丙型肝炎病毒相关的RNA依赖性RNA聚合酶活性的方法。 该方法的特征在于在反应混合物中使用包含在NS5B中的序列。 末端核苷酸转移酶活性，NS5B蛋白质的另一个性质也可以使用这种方法再现。 该方法利用了纯化至表观均一性或存在于产生过量生物体的提取物中的NS5B蛋白可以催化向外源或内源RNA分子的3'末端加入核糖核苷酸的事实。 本发明还涉及包含NS5B中包含的序列的物质组合物，以及这些组合物用于建立能够为治疗目的选择抑制与NS5B相关的酶活性的化合物的酶学试验的用途。
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3.发明授权Chimeric pestivirus with insertion in 3′ nontranslated region (3′NTR) with stable replication and rnase resistance 有权嵌合瘟病毒插入3'非翻译区（3'NTR），具有稳定的复制和抗性公开(公告)号：US08932606B2
公开(公告)日：2015-01-13
申请号：US13131027
申请日：2009-11-24
IPC分类号： A61K39/12 , A61K39/15 , A61K39/193 , A61K39/29 , C12N7/00
CPC分类号： C12N7/00 , C07H21/02 , C12N15/86 , C12N2770/24211 , C12N2770/24222 , C12N2770/24311 , C12N2770/24322
摘要： The construction of a chimeric Pestivirus by the identification of selected regions in the 3′NTR of the viral RNA genome is described where additional RNA sequences can be stably inserted. These sequence insertions in the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.
摘要翻译： 描述了通过鉴定病毒RNA基因组的3'NTR中的选定区域构建嵌合瘟病毒，其中可稳定插入另外的RNA序列。 病毒RNA基因组中的这些序列插入在复制中是稳定的并且能够形成感染性的RNA酶抗性病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。
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公开(公告)号：US20060110404A1
公开(公告)日：2006-05-25
申请号：US10532050
申请日：2003-09-12
申请人： Sven-Erik Behrens , Olaf Isken , Claus Grassmann , Robert Sarisky
发明人： Sven-Erik Behrens , Olaf Isken , Claus Grassmann , Robert Sarisky
CPC分类号： C12Q1/18 , C07K14/47 , G01N33/5308 , G01N33/6866 , G01N2333/085 , G01N2333/18
摘要： A method of modulating viral RNA replication and translation, in a eukaryotic cell, of positive-strand viral RNA, comprising the step of contacting a viral RNA-binding protein (vRbp) with a compound that modulates an activity of said protein.
摘要翻译： 调节正链病毒RNA在真核细胞中的病毒RNA复制和翻译的方法，包括使病毒RNA结合蛋白（vRbp）与调节所述蛋白质的活性的化合物接触的步骤。
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5.发明授权Replication stable and RNase resistant chimeras of pestivirus with insertion in 3′ nontranslated region (3′NTR) 有权具有插入3'非翻译区（3'NTR）的瘟病毒复制稳定型和RNA酶抗性嵌合体公开(公告)号：US08986673B2
公开(公告)日：2015-03-24
申请号：US13470148
申请日：2012-05-11
CPC分类号： C12N7/00 , C07H21/02 , C12N15/86 , C12N2770/24211 , C12N2770/24222 , C12N2770/24311 , C12N2770/24322
摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.
摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。
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6.发明申请Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR) 有权3'非翻译区插入的瘟病毒复制稳定型和RNA酶抗性嵌合体（3'NTR）公开(公告)号：US20120231539A1
公开(公告)日：2012-09-13
申请号：US13470148
申请日：2012-05-11
CPC分类号： C12N7/00 , C07H21/02 , C12N15/86 , C12N2770/24211 , C12N2770/24222 , C12N2770/24311 , C12N2770/24322
摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.
摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。
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公开(公告)号：US09885052B2
公开(公告)日：2018-02-06
申请号：US13129267
申请日：2009-11-13
申请人： Karin Breunig , Sven-Erik Behrens
发明人： Karin Breunig , Sven-Erik Behrens
IPC分类号： C12N1/19 , C12N15/81 , A61K39/12 , C07K14/005 , G01N33/569 , A61K39/00
CPC分类号： C12N15/815 , A61K39/00 , A61K39/12 , A61K2039/523 , A61K2039/542 , A61K2039/552 , C07K14/005 , C12N2770/24322 , C12N2770/24334 , G01N33/56983
摘要： Recombinant yeast cells are produced which are used for vaccination, among other uses for the oral vaccination by feeding.
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8.发明申请CHIMERIC PESTIVIRUS WITH INSERTION IN 3' NONTRANSLATED REGION (3' NTR) WITH STABLE REPLICATION AND RNASE RESISTANCE 有权具有稳定复制和RNA酶电阻的3'非翻译区（3'NTR）插入的重要农药公开(公告)号：US20110318813A1
公开(公告)日：2011-12-29
申请号：US13131027
申请日：2009-11-24
IPC分类号： C12N7/01
CPC分类号： C12N7/00 , C07H21/02 , C12N15/86 , C12N2770/24211 , C12N2770/24222 , C12N2770/24311 , C12N2770/24322
摘要： The construction of a chimeric Pestivirus by the identification of selected regions in the 3′NTR of the viral RNA genome is described where additional RNA sequences can be stably inserted. These sequence insertions in the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.
摘要翻译： 描述了通过鉴定病毒RNA基因组的3'NTR中的选定区域构建嵌合瘟病毒，其中可稳定插入另外的RNA序列。 病毒RNA基因组中的这些序列插入在复制中是稳定的并且能够形成感染性的RNA酶抗性病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。
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9.发明申请Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR) 审中-公开3'非翻译区插入的瘟病毒复制稳定型和RNA酶抗性嵌合体（3'NTR）公开(公告)号：US20100129902A1
公开(公告)日：2010-05-27
申请号：US12277282
申请日：2008-11-24
CPC分类号： C12N7/00 , C07H21/02 , C12N15/86 , C12N2770/24211 , C12N2770/24222 , C12N2770/24311 , C12N2770/24322
摘要： The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays.
摘要翻译： 本发明涉及核酸扩增领域，特别涉及用于病毒RNA测定的质量控制材料。 它具体涉及通过鉴定病毒RNA基因组的3'NTR中可以稳定插入附加顺序元件的区域来构建重组瘟病毒。 在病毒RNA基因组的3'非翻译区（3'NTR）中具有序列插入的嵌合瘟病毒在复制中是稳定的，并且能够形成感染性的，耐RNase的病毒颗粒。 这种具有3'NTR插入的嵌合瘟病毒可以用作RNA靶标的分析测定中的质量控制材料，包括外部，内部对照，PCR和NAT核酸测定中的定量标准。
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