Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis
    7.
    发明授权
    Adjustable sensitivity, genetic molecular interaction systems, including protein-protein interaction systems for detection and analysis 失效
    可调敏感性,遗传分子相互作用系统,包括用于检测和分析的蛋白质 - 蛋白质相互作用系统

    公开(公告)号:US06790607B1

    公开(公告)日:2004-09-14

    申请号:US09680738

    申请日:2000-10-06

    IPC分类号: C12Q100

    摘要: A method for detecting interactions between first and second interacting molecules at variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.

    摘要翻译: 一种以可变灵敏度检测第一和第二相互作用分子之间的相互作用的方法。 可以通过提供含有DNA结合结构域(脱敏)的诱饵杂交蛋白或含有报告基因的DNA活化域的猎物杂交蛋白(增强的灵敏度)来过表达。 可以容易地实现根据特定系统的需要使用一种或另一种外源活化剂。