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    SURFACE LINKERS FOR ARRAY SYNTHESIS
    1.
    发明申请
    SURFACE LINKERS FOR ARRAY SYNTHESIS 审中-公开
    表面连接器用于阵列合成

    公开(公告)号：US20130165350A1

    公开(公告)日：2013-06-27

    申请号：US13335677

    申请日：2011-12-22

    申请人： Robert Kuimelis ,  Glenn McGall ,  Dexter Pao ,  Zihui Chen ,  Trevor Axelrod

    发明人： Robert Kuimelis ,  Glenn McGall ,  Dexter Pao ,  Zihui Chen ,  Trevor Axelrod

    IPC分类号： C40B50/18

    CPC分类号： C40B50/18 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725

    摘要： The present invention provide several methods of derivatizing a surface of a support with one or more linkers thus providing a suitable platform for synthesis of a polymer array, particular a nucleic acid array. Some methods derivatize a surface with a self-assembled monolayer (SAM) of a linker. The SAM confers advantages of hydrolytic stability, broad compatibility with synthesis and detection chemistries, and reduced emergence of latent functional groups during polymer array synthesis. Substrates can also be derivatized with multi-layers of SAMs providing greater hydrolytic stability. Substrates can also be derivatized by synthesizing a linker in situ on the substrate by atom transfer radical polymerization of functional and functional monomers. Appropriate selection of monomers reduces emergence of latent functional groups in subsequent array synthesis.

    摘要翻译： 本发明提供了用一种或多种接头衍生载体表面的几种方法，从而提供合成聚合物阵列，特别是核酸阵列的合适平台。 一些方法用接头的自组装单层（SAM）衍生表面。 SAM赋予水解稳定性的优点，与合成和检测化学物质的广泛相容性，以及降低聚合物阵列合成期间潜在官能团的出现。 底物也可以通过提供更好的水解稳定性的多层SAM衍生化。 也可以通过功能单体和官能单体的原子转移自由基聚合在底物上原位合成接头来衍生底物。 合适的单体选择减少后续阵列合成中潜在官能团的出现。

    Oxide Layers on Silicon Substrates for Effective Confocal Laser Microscopy
    2.
    发明申请
    Oxide Layers on Silicon Substrates for Effective Confocal Laser Microscopy 有权
    硅衬底上的氧化物层用于有效的共焦激光显微镜

    公开(公告)号：US20070238185A1

    公开(公告)日：2007-10-11

    申请号：US11614896

    申请日：2006-12-21

    申请人： Robert Kuimelis ,  Zihui Chen ,  Glenn McGall

    发明人： Robert Kuimelis ,  Zihui Chen ,  Glenn McGall

    IPC分类号： G01N21/76 ,  G01N33/00 ,  G01N33/48

    CPC分类号： G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333 ,  Y10T436/25

    摘要： Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.

    摘要翻译： 在设置在硅晶片基板上的聚合物阵列上进行共焦激光显微镜的方法，所述方法包括以下步骤：提供具有顶侧和底侧的硅晶片衬底，用氧化物涂层涂覆硅晶片的顶侧 提供氧化物涂覆的晶片，将多个探针共价耦合到涂覆的晶片的顶侧，以提供固定的聚合物阵列，将固定的聚合物阵列与多个标记的配体杂交，以及使用共焦激光测定一个或多个杂交配体 提供荧光显微镜检测杂交。

    Cyclic silicon compounds
    7.
    发明申请
    Cyclic silicon compounds 审中-公开
    环状硅化合物

    公开(公告)号：US20050080284A1

    公开(公告)日：2005-04-14

    申请号：US10674711

    申请日：2003-09-29

    申请人： Robert Kuimelis ,  Glenn McGall

    发明人： Robert Kuimelis ,  Glenn McGall

    IPC分类号： C07F7/18 ,  C07F7/02 ,  C07F7/10 ,  C07F7/21

    CPC分类号： C07F7/1804

    摘要： The present invention provides cyclic silane based compounds. In accordance with one aspect of the present invention, it has been discovered that certain linear silane compounds can cyclize under some conditions. In one aspect of the present invention, cyclic silanes having the following formula are presented: In another aspect of the present invention, cyclic silanes having the following formula are presented:

    摘要翻译： 本发明提供了环状硅烷基化合物。 根据本发明的一个方面，已经发现某些线性硅烷化合物可以在某些条件下环化。 在本发明的一个方面，提出了具有下式的环状硅烷：在本发明的另一方面，提出了具有下式的环状硅烷：

    Photolithographic solid-phase polymer synthesis
    9.
    发明授权
    Photolithographic solid-phase polymer synthesis 有权
    光刻固相聚合物合成

    公开(公告)号：US07144700B1

    公开(公告)日：2006-12-05

    申请号：US09620780

    申请日：2000-07-21

    申请人： Glenn McGall ,  Daniel E. Falvey ,  Jacqueline A. Fidanza ,  Brian M. Feldman

    发明人： Glenn McGall ,  Daniel E. Falvey ,  Jacqueline A. Fidanza ,  Brian M. Feldman

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00 ,  G01N33/543

    CPC分类号： C07H21/00 ,  Y02P20/55 ,  Y10T436/17 ,  Y10T436/23

    摘要： Methods, employing a polycyclic hydrocarbon or a polycyclic heteroaromatic compound as sensitizers, are provided to increase the efficiency of removing, by irradiation, photolabile protecting groups that mask reactive sites on synthesis intermediaries. Preferred groups of photolabile protecting moieties include: ((α-methyl-2-nitropiperonyl)-oxy)carbonyl (MeNPOC), ((Phenacyl)-oxy)carbonyl (PAOC), O-(9-phenylxanthen-9-yl) (PIXYL), and ((2-methylene-9,10-anthraquinone)-oxy)carbonyl (MAQOC). In conjunction with using the sensitizers and protecting groups described above, a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support is provided. These resulting solid-phase arrays are useful, for example, to assay for the presence of biochemical products in biological samples.

    摘要翻译： 提供了使用多环烃或多环杂芳族化合物作为敏化剂的方法，以提高通过照射去除掩蔽合成中间体上反应性位点的光不稳定保护基的效率。 优选的光不稳定保护基团包括：（（α-甲基-2-硝基吡喃基） - 氧基）羰基（MeNPOC），（（苯甲酰基） - 氧基）羰基（PAOC），O-（9-苯基呫吨-9-基） PIXYL）和（（2-亚甲基-9,10-蒽醌） - 氧基）羰基（MAQOC）。 结合使用上述敏化剂和保护基团，提供了从组分分子在载体上形成多种化合物的方法，每种化合物占据支持体的单独的预定区域。 这些所得到的固相阵列可用于例如测定生物样品中生化产物的存在。

    Functionalized silicon compounds and methods for their synthesis and use
    10.
    发明授权
    Functionalized silicon compounds and methods for their synthesis and use 有权

    公开(公告)号：US07129307B2

    公开(公告)日：2006-10-31

    申请号：US10853378

    申请日：2004-05-25

    申请人： Glenn McGall ,  Jonathan Eric Forman

    发明人： Glenn McGall ,  Jonathan Eric Forman

    IPC分类号： C08G77/00 ,  C08G77/04

    CPC分类号： C08G73/0206 ,  C03C17/30 ,  C03C2218/113 ,  C07F7/1804 ,  C09D179/02 ,  C09D183/08 ,  C09D183/14 ,  Y10T428/31663

    摘要： Provided are functionalized silicon compounds and methods for their synthesis and use. The functionalized silicon compounds include at least one activated silicon group and at least one derivatizable functional group. Exemplary derivatizable functional groups include hydroxyl, amino, carboxyl and thiol, as well as modified forms thereof, such as activated or protected forms. The functionalized silicon compounds may be covalently attached to surfaces to form functionalized surfaces which may be used in a wide range of different applications. In one embodiment, the silicon compounds are attached to the surface of a substrate comprising silica, such as a glass substrate, to provide a functionalized surface on the substrate, to which molecules, including polypeptides and nucleic acids, may be attached. In one embodiment, after covalent attachment of a functionalized silicon compound to the surface of a solid silica substrate to form a functionalized coating on the substrate, an array of nucleic acids may be covalently attached to the substrate. Thus, the method permits the formation of high density arrays of nucleic acids immobilized on a substrate, which may be used, for example, in conducting high volume nucleic acid hybridization assays.