公开(公告)号：US11385165B2

公开(公告)日：2022-07-12

申请号：US16087176

申请日：2017-03-10

Applicant: Sony Corporation

Inventor： Yoichi Katsumoto ,  Masahiro Matsumoto ,  Kazuya Takahashi

IPC: G01N15/14 ,  B01L3/00 ,  G01N15/10

Abstract: According to some aspects, a sample isolation kit is provided. The sample isolation kit includes a housing configured to detachably couple to a sample fluid channel of a microchip and provide a sample to the microchip. The housing and the microchip are coupled using a hermetic seal. The sample isolation kit further includes a storage housing configured to detachably couple to an isolation channel of the microchip and receive a target biological sample isolated from the sample by the microchip. The storage housing and the microchip are coupled using a hermetic seal.

公开(公告)号：US11199541B2

公开(公告)日：2021-12-14

申请号：US16065947

申请日：2016-08-04

Applicant: Sony Corporation

Inventor： Masahiro Matsumoto ,  Masataka Shinoda ,  Yuuki Watanabe

IPC: G01N33/569 ,  G01N33/532 ,  G01N33/53

Abstract: According to some aspects, a method for analyzing a cell is provided. The method includes trapping the cell by binding a first molecule to the cell. The method further includes binding a second molecule to the cell. The second molecule includes a binding portion capable of specific binding to a cell-surface molecule of the cell. The second molecule further includes an identifying portion, a labeling portion coupled to the identifying portion, and a stimulus-degradable linker between the binding portion and the identification portion. The method further includes detaching the identifying portion from the binding portion by stimulating the stimulus-degradable linker where the detached identifying portion is coupled to the labeling portion. The method further includes binding the detached identifying portion through specific binding to an identifying portion recognizing molecule and detecting the labeling portion.

公开(公告)号：US20210170402A1

公开(公告)日：2021-06-10

申请号：US16762295

申请日：2018-11-09

Applicant: Sony Corporation

Inventor： Tatsumi Ito ,  Kazuya Takahashi ,  Masahiro Matsumoto

IPC: B01L3/00 ,  G01N15/14

Abstract: A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows.

公开(公告)号：US20190264159A1

公开(公告)日：2019-08-29

申请号：US16087385

申请日：2017-02-17

Applicant: Sony Corporation

Inventor： Masahiro Matsumoto

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12N5/00

Abstract: To provide a cell culture container and a cell culture system that can perform cell sorting, culturing, cell processing, and the like in one space and a volume of the space can be varied to suit respective steps.A cell culture container includes first molecules each bondable to target cells to be cultured, being immobilized to the container via a stimulus degradable linker, the container having a variable volume.A cell culture system included a cell culture container, including first molecules each bondable to target cells to be cultured, being immobilized to the container via a stimulus degradable linker, the container having a variable volume, and a stimulus imparting device that imparts a stimulus to the stimulus degradable linker

公开(公告)号：US20140134077A1

公开(公告)日：2014-05-15

申请号：US14073382

申请日：2013-11-06

Applicant: Sony Corporation

Inventor： Tasuku Yotoriyama ,  Yuji Segawa ,  Michihiro Ohnishi ,  Yoshiaki Kato ,  Tomoteru Abe ,  Kenzo Machida ,  Masahiro Matsumoto ,  Tomohiko Nakamura ,  Naohisa Sakamoto ,  Hidetoshi Watanabe ,  Takanori Anaguchi ,  Masayoshi Akita ,  Masahiro Miyachi

IPC: B01L3/02 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L7/00 ,  B01L2200/027 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0835 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/049

Abstract: There is provided a sample liquid injection tool including a reservoir section configured to store a sample liquid, a channel having one end protruding from an outer surface and configured to discharge the sample liquid therein from a protrusion end to an outside, and a heating unit and a filter installed between the reservoir section and the channel to enable passage of the liquid.

Abstract translation: 提供了一种样品液体注入工具，其包括：储存部，其构造成存储样品液体;通道，其一端从外表面突出，并且构造成将其中的样品液体从突出端排出到外部;以及加热单元， 安装在储存器部分和通道之间以使液体通过的过滤器。

公开(公告)号：US20130102062A1

公开(公告)日：2013-04-25

申请号：US13655156

申请日：2012-10-18

Applicant: Sony Corporation

Inventor： Kensuke Kojima ,  Masaki Sato ,  Masahiro Matsumoto

IPC: C12M1/40

CPC classification number: B01L7/52 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/161 ,  C12Q1/6844 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Provided is a microchip for a nucleic acid amplification reaction, including a well configured to function as a reaction site of the nucleic acid amplification reaction, and the well has a center portion and a marginal portion, a substance anchored in a form that the substance is eccentrically-located less at the center portion and much at the marginal portion of the well, in which the substance is at least a part of a substance for the nucleic and amplification reaction.

Abstract translation: 本发明提供了用于核酸扩增反应的微芯片，其包括配置成作为核酸扩增反应的反应位点的阱，并且所述阱具有中心部分和边缘部分，所述物质以所述物质的形式被锚定 偏心位于较小的中心部分处，并且远在井的边缘部分，其中物质是用于核酸和扩增反应的物质的至少一部分。

公开(公告)号：US12122988B2

公开(公告)日：2024-10-22

申请号：US16978153

申请日：2019-01-17

Applicant: SONY CORPORATION

Inventor： Shiori Oshima ,  Koji Futamura ,  Kenji Tanaka ,  Tomoyuki Umetsu ,  Masahiro Matsumoto

IPC: C12M1/34 ,  G01N21/76

CPC classification number: C12M1/34 ,  G01N21/76

Abstract: To provide a cell evaluation device capable of evaluating cells and a secretory substance emitted from the cells accurately and precisely. Provided is a cell evaluation device including a first capturing unit that captures a secretory substance secreted by cells, and an image acquisition unit that acquires an image obtained by imaging observation light of the cells and light emitted from a luminescent substance bonded to the secretory substance, in which the image acquisition unit and the first capturing unit are disposed in this order, and the image acquisition unit acquires position information for the cells and the secretory substance.

公开(公告)号：US11440004B2

公开(公告)日：2022-09-13

申请号：US16762295

申请日：2018-11-09

Applicant: Sony Corporation

Inventor： Tatsumi Ito ,  Kazuya Takahashi ,  Masahiro Matsumoto

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

Abstract: A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows.

公开(公告)号：US09545630B2

公开(公告)日：2017-01-17

申请号：US14378588

申请日：2013-01-16

Applicant: Sony Corporation

Inventor： Masahiro Matsumoto ,  Masaki Sato ,  Hidetoshi Watanabe

IPC: C12Q1/68 ,  B01J19/00 ,  B01L3/00 ,  C12N15/10

CPC classification number: B01L3/502707 ,  B01L3/5027 ,  B01L3/5088 ,  C12N15/10 ,  C12Q1/6844

Abstract: Provided is a method for fabricating a microchip for nucleic acid amplification reaction that is capable of simple and highly accurate analysis. Provided is a method for fabricating a microchip for nucleic acid amplification reaction, the method including a solidification step of drying a reagent solution including at least a part of substances required for a nucleic acid amplification reaction, and a containment step of arranging the reagent solution including the solidified substance in wells that serve as a reaction site for a nucleic acid amplification reaction. In the microchip for nucleic acid amplification reaction fabricated by the fabrication method, since substances required for the nucleic acid amplification reaction are contained by being solidified, non-specific amplification is suppressed in a nucleic acid amplification reaction, which enables highly accurate analysis.

Abstract translation: 提供一种能够进行简单高精度分析的核酸扩增反应用微芯片的制造方法。 提供一种用于制造用于核酸扩增反应的微芯片的方法，所述方法包括：干燥包含核酸扩增反应所需的至少一部分物质的试剂溶液的固化步骤;以及包含步骤， 用作核酸扩增反应的反应位点的孔中的固化物质。 在通过制造方法制造的用于核酸扩增反应的微芯片中，由于通过固化而含有核酸扩增反应所需的物质，因此在核酸扩增反应中抑制非特异性扩增，能够进行高精度的分析。

公开(公告)号：US20150004676A1

公开(公告)日：2015-01-01

申请号：US14375315

申请日：2013-01-30

Applicant: Sony Corporation

Inventor： Masahiro Matsumoto ,  Michihiro Ohnishi ,  Yoshiaki Kato ,  Toshio Watanabe

IPC: G01N1/28

CPC classification number: G01N1/28 ,  B01L3/50273 ,  B01L2300/0627 ,  B01L2400/049

Abstract: A microchip is provided and configured to contain a sample solution for analysis. The microchip including a channel that is maintained at a pressure level less than atmospheric pressure so as to allow flow of the sample solution thru the channel; and a pressure indication section configured to detect a change in the pressure level. A microchip apparatus and a method of manufacturing a microchip are also provided.

Abstract translation: 微芯片被提供并配置成容纳用于分析的样品溶液。 微芯片包括保持在低于大气压力的压力水平的通道，以允许样品溶液流过通道; 以及构造成检测压力水平的变化的压力指示部。 还提供了微芯片装置和微芯片的制造方法。